Results 131 to 140 of about 184,302 (307)

EXPRESSION OF Gag AND Gag-Pol OF BOVINE IMMUNODEFICIENCY VIRUS

open access: yes, 1998
The gag and gag-pol genes of bovine immunodeficiency virus (BIV) were expressed and characterized in the baculovirus expression vector system. It was known that retrovirus gag gene products alone were capable of assembly and production of virus-like ...
Rasmussen, Lynn
core   +1 more source

Majority‐Voting Overlapping Method for Error Correction in DNA Data Storage

open access: yesAdvanced Intelligent Discovery, EarlyView.
We propose an overlapping‐based majority‐voting method for DNA data storage error correction. By aligning multiple reads and choosing the most frequent base per position, it suppresses substitution errors without prior models. Validated on synthetic and real sequencing data, it achieves high‐fidelity, scalable, and cost‐effective reconstruction ...
Thi Bich Ngoc Nguyen   +5 more
wiley   +1 more source

A virus-like particle assembly system for probing the HIV-1 Gag–Pol dimerization domain: supporting evidence for reverse transcriptase involvement in protease activation by influencing Gag–Pol/Gag–Pol interaction

open access: yesJournal of Virology
HIV-1 protease (PR) activation is triggered by Gag–Pol dimerization. We previously reported that reverse transcriptase (RT) amino acid substitution mutations resulted in p66/51RT heterodimer instability associated with impaired PR activation, and that ...
Shih-Han Hsieh   +2 more
doaj   +1 more source

Association of HIV-1 gag with LAMP-1 does not protect Gag protein from degradation, but increases Gag mRNA production.

open access: yes, 2014
A) HEK 293 cells were transfected with native gag (gagN) or LAMP/gag. After 48 hours, the expression of Gag protein was analyzed by western blotting, by staining with mouse anti-Gag antibody, followed by HRP-conjugated anti-mouse IgG.
Jorge Luiz Santos Gonçalves (578468)   +10 more
core   +1 more source

The psychosocial toll of Dublin III on asylum seekers in the Netherlands

open access: yesAmerican Journal of Community Psychology, EarlyView.
Abstract The Dublin III Regulation determines which EU Member State is responsible for examining asylum claims, but its implementation carries significant consequences for those subjected to it. This study examines how Dublin III, as implemented in the Netherlands, affects asylum seekers' psychosocial wellbeing using Silove′s Adaptation and Development
Imen El Amouri
wiley   +1 more source

Rhenium‐Based β‐Galactosidase Probe Allows Senescence Profiling by Mass Cytometry

open access: yesAngewandte Chemie, EarlyView.
Senescence‐associated β‐galactosidase (SA‐β‐Gal) activity is a widely accepted biomarker of cellular senescence, yet its integration with other senescence markers has been limited by available detection methods. Here, we introduce ReGal, a mass cytometry‐compatible SA‐β‐Gal activity probe that can be multiplexed with established markers of senescence ...
Adam Tam   +4 more
wiley   +2 more sources

Protection from a Vaccinia Virus-Gag challenge by BCGpan-Gag.

open access: yes, 2013
Challenge of mice vaccinated with BCGpan-Gag or BCGpan-Control vaccine with VV-Gag or VV-NYCBH. Groups of 10 mice were vaccinated with BCGpan-Gag (2×106 cfu), BCGpan-Control (2×106 cfu) or resupension medium on day 0 and day 28.
Vasan Sambandamurthy (327983)   +8 more
core   +1 more source

A Novel NR3C1 Frameshift Variant Associated With Familial Glucocorticoid Resistance Syndrome: An Integrated Analysis of Steroid Profiling and Structural Modeling

open access: yesAmerican Journal of Medical Genetics Part A, EarlyView.
ABSTRACT Glucocorticoid resistance syndrome (GRS) is a rare hereditary disorder caused by pathogenic variants in NR3C1, characterized by marked phenotypic heterogeneity and frequent misdiagnosis as primary aldosteronism or subclinical Cushing's syndrome.
Sufang Yun   +7 more
wiley   +1 more source

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