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Capillary Sodium Dodecyl Sulfate Agarose Gel Electrophoresis of Proteins [PDF]

open access: yesGels, 2022
Capillary sodium dodecyl sulfate gel electrophoresis has long been used for the analysis of proteins, mostly either with entangled polymer networks or translationally cross-linked gels.
Daniel Sarkozy, Andras Guttman
doaj   +2 more sources

Improved proteomic analysis of nuclear proteins, as exemplified by the comparison of two myelo\"id cell lines nuclear proteomes [PDF]

open access: yesJournal of Proteomics (2012) epub ahead of print, 2012
One of the challenges of the proteomic analysis by 2D-gel is to visualize the low abundance proteins, particularly those localized in organelles. An additional problem with nuclear proteins lies in their strong interaction with nuclear acids.
Chevallet, Mireille   +5 more
core   +5 more sources

Power and limitations of electrophoretic separations in proteomics strategies [PDF]

open access: yesMass Spectrometry Reviews 28, 5 (2009) 816-43, 2009
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry.
Aebersold   +225 more
core   +4 more sources

Two-dimensional gel electrophoresis in proteomics: A tutorial [PDF]

open access: yesJournal of Proteomics 74, 10 (2011) 1829-41, 2011
Two-dimensional electrophoresis of proteins has preceded, and accompanied, the birth of proteomics. Although it is no longer the only experimental scheme used in modern proteomics, it still has distinct features and advantages.
##Righetti   +108 more
core   +4 more sources

Mitochondrial proteomics: analysis of a whole mitochondrial extract with two-dimensional electrophoresis [PDF]

open access: yesMethods in molecular biology (Clifton, N.J.) 432 (2008) 83-100, 2008
Mitochondria are complex organelles, and their proteomics analysis requires a combination of techniques. The emphasis in this chapter is made first on mitochondria preparation from cultured mammalian cells, then on the separation of the mitochondrial ...
A. Gorg   +16 more
core   +6 more sources

Elution of proteins from polyacrylamide eels: a simple and economic procedure [PDF]

open access: yesRevista do Instituto de Medicina Tropical de São Paulo, 1989
A simplified methodology for the quantitative electroelution of proteins from polyacrylamide gels is described. After staining with Coomassie Brilliant Blue R 250, the identified bands are excised from the gel and the proteins eluted using a procedure ...
Vera Bongertz
doaj   +6 more sources

Membrane proteins and proteomics: Love is possible, but so difficult [PDF]

open access: yesElectrophoresis 30, S1 (2009) S174-S180, 2009
Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with ...
Adessi   +103 more
core   +5 more sources

Electrophoretic Separation of the Soluble Protein of Sclerotinia sclerotiorum, S. trifoliorum and S. Minor [PDF]

open access: yesJournal of Mathematical and Fundamental Sciences, 2019
. Soluble proteins extracted from 3 species of Sclerotinia sclerotiorum, S. trifoliorum and S. minor were separated on polyacrylamide gel electrophoresis. The three species of Sclerotinia could be distinguished by their protein patterns.
Buddy Dani Kosasih
doaj   +2 more sources

Antigenic identity of immunodominant proteins of Bacillus anthracis genovariants

open access: yesЖурнал микробиологии, эпидемиологии и иммунобиологии, 2023
Introduction. The main biological raw materials for the production of immunobiological preparations for identification of Bacillus anthracis are its specific antigens, the protective antigen and the EA1 protein. Purpose.
Margarita P. Izhberdeeva   +3 more
doaj   +1 more source

Analysis on Gel Electrophoresis in Biology [PDF]

open access: yesE3S Web of Conferences, 2020
Gel electrophoresis is one of the basic methods used in modern biological laboratories to separate different compounds in a mixture. It is widely used in analyzing the amino acid sequence in proteins.
Cai Yiran
doaj   +1 more source

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