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Gel Electrophoresis of Proteins [PDF]

, 2012
Gel electrophoresis is a widely known group of techniques used to separate and identify macromolecules as DNA, RNA, or proteins based on size, form, or isoelectric point. The separation of molecules by electrophoresis is based on the fact that charged molecules migrate through a gel matrix upon application of an electric field.
Alberto Alcázar, Cristina Cid, Eva García-López, Fernando Baquero, Laura García-Descalzo   +4 more
core   +7 more sources

Capillary Sodium Dodecyl Sulfate Agarose Gel Electrophoresis of Proteins [PDF]

Gels, 2022
Capillary sodium dodecyl sulfate gel electrophoresis has long been used for the analysis of proteins, mostly either with entangled polymer networks or translationally cross-linked gels.
Daniel Sarkozy, Andras Guttman
doaj   +2 more sources

Improved proteomic analysis of nuclear proteins, as exemplified by the comparison of two myeloïd cell lines nuclear proteomes [PDF]

Journal of Proteomics (2012) epub ahead of print, 2012
One of the challenges of the proteomic analysis by 2D-gel is to visualize the low abundance proteins, particularly those localized in organelles. An additional problem with nuclear proteins lies in their strong interaction with nuclear acids. Several experimental procedures have been tested to increase, in the nuclear extract, the ratio of nuclear ...
Chevallet, Mireille, Diemer, Hélène, Lelong, Cécile, Luche, Sylvie, Rabilloud, Thierry, Van Dorsselaer, Alain   +5 more
arxiv   +5 more sources

Power and limitations of electrophoretic separations in proteomics strategies [PDF]

Mass Spectrometry Reviews 28, 5 (2009) 816-43, 2009
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry. These techniques, dealing with proteins or peptides, can rely on chromatography or electrophoresis.
Aebersold, An, Andersen, Anderson, Anderson, Anderson, Anderson, Anderson, Appel, Artoni, Baczek, Baczek, Bell, Berggren, Bjellqvist, Bjellqvist, Bonner, Braun, Bravo, Bravo, Bravo, Bravo, Brown, Bunai, Bunger, Burre, Bury, Caglio, Caglio, Camacho-Carvajal, Cantin, Cargile, Cargile, Cargile, Castellanos-Serra, Cejka, Chen, Chevalier, Chevallet, Chiari, Chiari, Chick, Claverol, Cleveland, Cohen, Colinge, Corbett, Coughenour, Courchesne, Cox, Daban, Daban, Davis, Domon, Dukan, Duncan, Egen, Eng, Eravci, Essader, Fernandez-Patron, Figeys, Fritz, Garrels, Garrels, Gelfi, Gelfi, Giometti, Gordon, Gordon, Gorg, Gorg, Gorg, Groleau, Gronow, Han, Hardison, Hartinger, Heller, Henrich, Henzel, Hisabori, Hoffert, Hoffmann, Horowitz, Horth, Hunzinger, James, Johnson, Johnston, Jorgensen, Jovin, Jovin, Kasicka, Keller, Kobayashi, Krijgsveld, Krokhin, Kuroda, Kuster, Kyte, Laemmli, Lambert, Langen, Lasonder, Lengqvist, Liang, Link, Liu, Locke, Loo, Lopez, Lu, Lu, Lu, Luche, Lui, Luo, Luque-Garcia, Lyubimova, Macfarlane, Macfarlane, MacGillivray, Mackintosh, Malmstrom, Malone, Matsudaira, Mazzeo, Mets, Moritz, Nakamura, Navarre, Neuhoff, Nielsen, Novakova, O'Farrell, O'Farrell, Ogorzalek Loo, Orrick, Ortiz, Oses-Prieto, Pappin, Patton, Peng, Perkins, Petritis, Phelps, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Radola, Radola, Rais, Reid, Reynolds, Richert, Rigaut, Righetti, Righetti, Righetti, Righetti, Righetti, Ros, Rosenfeld, Sanchez, Santoni, Schagger, Schagger, Scherl, Schirle, Schluesener, Schmitt, Sevinsky, Sevinsky, Shang, Shapiro, Shen, Shimura, Shimura, Siemankowski, SimoAlfonso, Simpson, Steinberg, Steinberg, Switzer, Szponarski, Szponarski, Tabb, Tan, Tarroux, Tastet, Towbin, Tsugita, Tuszynski, Unlu, van Oostveen, Vonguyen, Voris, Wang, Wang, Washburn, Weber, Wen, West, White, Wilkins, Williams, Wiltfang, Winkler, Wise, Witze, Xiao, Xie, Xie, Yamaguchi, Yamaguchi, Yata, Yates, Young, Yu, Yuan, Zahedi, Zewert, Zischka, Zuo   +225 more
arxiv   +5 more sources

Two-dimensional gel electrophoresis in proteomics: A tutorial [PDF]

Journal of Proteomics 74, 10 (2011) 1829-41, 2011
Two-dimensional electrophoresis of proteins has preceded, and accompanied, the birth of proteomics. Although it is no longer the only experimental scheme used in modern proteomics, it still has distinct features and advantages. The purpose of this tutorial paper is to guide the reader through the history of the field, then through the main steps of the
##Righetti, ##Righetti, ##Westermeier, #Celis, Afjehi-Sadat, Aicher, Anderson, Appel, Bravo, Bredemeyer, Burre, Cejka, Celis, Chevalier, Chevallet, Chevallet, Chevallet, Corbett, Cécile Lelong, Damerval, Eravci, Garrels, Ghaemmaghami, Gianazza, Gordon, Gorg, Gorg, Gravel, Gronow, Hackett, Harder, Hartinger, Ingolia, Ishigami, Ivanov, John, Karp, Klose, Kondo, Laemmli, Lelong, Lelong, Lovato, Lu, Luche, Luche, Maltese, Mechin, Nakamura, Olsson, Orrick, Perdew, Perluigi, Perrot, Pitarch, Pun, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Rasmussen, Richert, Righetti, Sanchez-Campillo, Semple-Rowland, Seo, Sinha, Sobel, Tannu, Tarroux, Tarroux, Taylor, Thierry Rabilloud, Tuszynski, Viswanathan, Wen, Wildgruber, Wildgruber, Yang, Zhan, Zhou, ⁎Gorg, ⁎Gorg, ⁎Hecker, ⁎Miller, ⁎Miller, ⁎Minden, ⁎Patton, ⁎Petrak, ⁎Rabilloud, ⁎Rabilloud, ⁎Rabilloud, ⁎Righetti, ⁎Wang, ⁎Westermeier, ⁎⁎Aebersold, ⁎⁎Anderson, ⁎⁎Anderson, ⁎⁎Bravo, ⁎⁎Gorg, ⁎⁎James, ⁎⁎MacGillivray, ⁎⁎Matsudaira, ⁎⁎Neuhoff, ⁎⁎O'Farrell, ⁎⁎Rabilloud, ⁎⁎Rosenfeld, ⁎⁎Unlu, ⁎⁎Wilkins   +108 more
arxiv   +5 more sources

Electrophoresis of Unlabeled Proteins in a Sequencing Gel Apparatus [PDF]

BioTechniques, 2000
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Claudio Luparello
doaj   +4 more sources

Elution of proteins from polyacrylamide eels: a simple and economic procedure [PDF]

Revista do Instituto de Medicina Tropical de São Paulo, 1989
A simplified methodology for the quantitative electroelution of proteins from polyacrylamide gels is described. After staining with Coomassie Brilliant Blue R 250, the identified bands are excised from the gel and the proteins eluted using a procedure ...
Vera Bongertz
doaj   +6 more sources

Membrane proteins and proteomics: Love is possible, but so difficult [PDF]

Electrophoresis 30, S1 (2009) S174-S180, 2009
Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with different proteins, while most protein chemistry methods work mainly, if not only, in water-based ...
Adessi, Aebersold, Aivaliotis, Aivaliotis, Alla, Ames, Babu, Bagshaw, Bell, Berlanda-Scorza, Berven, Bierczynska-Krzysik, Bisle, Bjellqvist, Bunai, Bunai, Burré, Buxbaum, Casey, Chevallet, Chevallet, Connolly, Coughenour, Cutillas, Devreese, Eravci, Everberg, Felsted, Ferro, Gatlin, Ghosh, Gyenes, Görg, Hagan, Hahne, Hartinger, Helling, Henningsen, Henzel, Hisabori, Hjelmeland, Huang, Huang, James, Jun, Lasonder, Lescuyer, Liberatori, Lopez, Low, Luche, Lyubimova, Lüddens, MacFarlane, MacFarlane, Matsudaira, McDonough, Miguet, Moebius, Molloy, Molloy, Nandakumar, Natera, Navarre, Ong, Papasotiriou, Pappin, Patton, Pedersen, Peng, Perdew, Phadke, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Rais, Rubin, Santoni, Santoni, Schindler, Schirle, Schägger, Siroy, Speers, Sweetnam, Switzer, Tastet, Taylor, Towbin, Veith, Washburn, Werner, Wilkins, Williams, Williams, Williams, Wittig, Witzmann, Wu, Yates, Ying, Zahedi   +103 more
arxiv   +5 more sources

Two-dimensional polyacrylamide gel electrophoresis of proteins synthesized and released by conceptuses and endometria from pony mares [PDF]

Journal of Reproduction and Fertility, 1990
Conceptuses were obtained from pony mares on each day of pregnancy between Days 12 and 28, and on Days 39, 45, 65 and 100. Endometrium was obtained from mares at Days 12, 14, 16, 18, 39, 45, 65 and 100 of pregnancy, and from non-pregnant mares during ...
K. J. McDowell, Dan C. Sharp, A.T. Fazleabas, R. M. Roberts   +3 more
openalex   +2 more sources

Electrophoretic Separation of the Soluble Protein of Sclerotinia sclerotiorum, S. trifoliorum and S. Minor [PDF]

Journal of Mathematical and Fundamental Sciences, 2019
. Soluble proteins extracted from 3 species of Sclerotinia sclerotiorum, S. trifoliorum and S. minor were separated on polyacrylamide gel electrophoresis. The three species of Sclerotinia could be distinguished by their protein patterns.
Buddy Dani Kosasih
doaj   +2 more sources