Results 11 to 20 of about 3,553,338 (335)

ELECTROPHORETIC SYSTEMS FOR PREPARATIVE FRACTIONATION OF PROTEIN PRECURSORS OF BIOACTIVE PEPTIDES FROM COW’S MILK

open access: yesHarčova Nauka ì Tehnologìâ, 2018
The article considers the possibility of obtaining purified fractions-precursors of bioactive peptides from milk proteins by the method of preparative electrophoresis.
V. Yukalo   +3 more
doaj   +1 more source

Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) [PDF]

open access: yes, 2008
QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point.
Bernd Kastenholz
core   +2 more sources

Polyacrylamide gel electrophoresis of soybean seed proteins

open access: yesActa Societatis Botanicorum Poloniae, 2015
Four major and 14 minor protein bands were detected when total salt soluble proteins of soybean (Glycine max cultivar Warszawska) seed were subjected to polyacrylamide gel electrophoresis under nondissociating conditions, and 16 protein bands were ...
W. Lassocińsk, J. S. Knypl
doaj   +1 more source

An Improved Method for PAGE-based Detection of Phosphorylated Protein in Yeast

open access: yesBio-Protocol, 2012
One dimensional polyacrylamide gel electrophoresis has been successfully used to detect protein phosphorylation. This method is very simple and highly reproducible.
Yuehua Wei
doaj   +1 more source

The guideline for western blotting assay

open access: yesFood Frontiers, 2022
Western blotting is a technique for detecting protein developed based on protein electrophoresis separation and antigen‐antibody detection. It combines the high resolution of SDS polyacrylamide gel electrophoresis with the specificity of antigen‐antibody
Fang Wang   +5 more
doaj   +1 more source

Resolving acetylated and phosphorylated proteins by neutral urea Triton-polyacrylamide gel electrophoresis: NUT-PAGE

open access: yesBioTechniques, 2014
Protein acetylation and phosphorylation are key modifications that regulate both normal and pathological protein functions. The gel systems currently used for analyzing modified proteins require either expensive reagents or time-consuming second ...
Christopher J. Buehl   +6 more
doaj   +1 more source

Obtaining Of β-Lactoglobulin By Gel Filtration Of Cow Milk Whey [PDF]

open access: yes, 2019
Milk whey proteins carry out a number of important biological functions and also they are precursors of many biologically active peptides (antihypertensive peptides, antagonists of opioid receptors, regulators of intestinal motility, immunomodulatory ...
Datsyshyn, K. (Kateryna)   +2 more
core   +3 more sources

Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients [PDF]

open access: yesElectrophoresis 18 (31/03/1997) 307-16, 2006
Membrane and nuclear proteins of poor solubility have been separated by high resolution two-dimensional (2-D) gel electrophoresis. Isoelectric focusing with immobilized pH gradients leads to severe quantitative losses of proteins in the resulting 2-D map, although the resolution is usually high.
arxiv   +1 more source

Protein electrophoretic migration data from custom and commercial gradient gels

open access: yesData in Brief, 2016
This paper presents data related to the article “A method for easily customizable gradient gel electrophoresis” (A.J. Miller, B. Roman, E.M. Norstrom, 2016) [1].
Andrew J. Miller   +2 more
doaj   +1 more source

Study of protein expresion [sic] in peri-infarct tissue after cerebral ischemia [PDF]

open access: yes, 2015
In this work, we report our study of protein expression in rat peri-infarct tissue, 48 h after the induction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis with mass spectrometry and combined fractional diagonal ...
Agulla, Jesús   +9 more
core   +5 more sources

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