Results 11 to 20 of about 3,475,365 (278)
Gel Electrophoresis of Proteins [PDF]
Gel electrophoresis is a widely known group of techniques used to separate and identify macromolecules as DNA, RNA, or proteins based on size, form, or isoelectric point. The separation of molecules by electrophoresis is based on the fact that charged molecules migrate through a gel matrix upon application of an electric field.
Alberto Alcázar+4 more
core +6 more sources
Electrophoresis of Unlabeled Proteins in a Sequencing Gel Apparatus [PDF]
n ...
Claudio Luparello
doaj +4 more sources
Protein purification by Off-Gel electrophoresis [PDF]
A novel free-flow protein purification technique based on isoelectric electrophoresis is presented, where the proteins are purified in solution without the need of carrier ampholytes. The gist of the method is to flow protein solutions under an immobilised pH gradient gel (IPG) through which an electric field is applied perpendicular to the direction ...
Ros, A.+8 more
openaire +5 more sources
Discontinuous native protein gel electrophoresis [PDF]
AbstractAnalysis of the oligomeric state of a native protein usually requires analytical ultracentrifugation or repeated gel filtration to calculate the protein's size. We have developed a discontinuous native protein gel electrophoresis system that allows the separation of even basic proteins according to their size, oligomeric state, and shape.
Michael Niepmann, Junfeng Zheng
openaire +3 more sources
Acute-phase protein concentration in bronchoalveolar lavage fluid from healthy horses [PDF]
: Bronchoalveolar lavage fluid (BALF) was analyzed to obtain information on leakage of acute-phase proteins from the blood into the respiratory lumen and about local synthesis.
Paula Alessandra Di Filippo+4 more
doaj +1 more source
Identification of Structural Proteins of Rhizobium meliloti Temperate Phage 16-3 [PDF]
The structural proteins of Rhizobium meliloti temperate phage 16-3 have been analysed by means of polyacrylamide gel electrophoresis, isoelectric focusing and agarose gel electrophoresis.
Duda, Ernő+3 more
core +1 more source
The article considers the possibility of obtaining purified fractions-precursors of bioactive peptides from milk proteins by the method of preparative electrophoresis.
V. Yukalo+3 more
doaj +1 more source
Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) [PDF]
QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point.
Bernd Kastenholz
core +2 more sources
Polyacrylamide gel electrophoresis of soybean seed proteins
Four major and 14 minor protein bands were detected when total salt soluble proteins of soybean (Glycine max cultivar Warszawska) seed were subjected to polyacrylamide gel electrophoresis under nondissociating conditions, and 16 protein bands were ...
W. Lassocińsk, J. S. Knypl
doaj +1 more source
An Improved Method for PAGE-based Detection of Phosphorylated Protein in Yeast
One dimensional polyacrylamide gel electrophoresis has been successfully used to detect protein phosphorylation. This method is very simple and highly reproducible.
Yuehua Wei
doaj +1 more source