Results 11 to 20 of about 864,514 (341)
Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) [PDF]
, 2008 QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point.
Bernd Kastenholz
core +2 more sources
An Improved Method for PAGE-based Detection of Phosphorylated Protein in Yeast
Bio-Protocol, 2012 One dimensional polyacrylamide gel electrophoresis has been successfully used to detect protein phosphorylation. This method is very simple and highly reproducible.
Yuehua Wei
doaj +1 more source
The guideline for western blotting assay
Food Frontiers, 2022 Western blotting is a technique for detecting protein developed based on protein electrophoresis separation and antigen‐antibody detection. It combines the high resolution of SDS polyacrylamide gel electrophoresis with the specificity of antigen‐antibody
Fang Wang +5 more
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Improved proteomic analysis of nuclear proteins, as exemplified by the comparison of two myelo\"id cell lines nuclear proteomes [PDF]
, 2012 One of the challenges of the proteomic analysis by 2D-gel is to visualize the low abundance proteins, particularly those localized in organelles. An additional problem with nuclear proteins lies in their strong interaction with nuclear acids.
Chevallet, Mireille +5 more
core +4 more sources
Study of protein expresion [sic] in peri-infarct tissue after cerebral ischemia [PDF]
, 2015 In this work, we report our study of protein expression in rat peri-infarct tissue, 48 h after the induction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis with mass spectrometry and combined fractional diagonal ...
Agulla, Jesús +9 more
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BioTechniques, 2014 Protein acetylation and phosphorylation are key modifications that regulate both normal and pathological protein functions. The gel systems currently used for analyzing modified proteins require either expensive reagents or time-consuming second ...
Christopher J. Buehl +6 more
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Protein electrophoretic migration data from custom and commercial gradient gels
Data in Brief, 2016 This paper presents data related to the article “A method for easily customizable gradient gel electrophoresis” (A.J. Miller, B. Roman, E.M. Norstrom, 2016) [1].
Andrew J. Miller +2 more
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Western blotting of native proteins from agarose gels
BioTechniques, 2022 We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid.
Chiaki Sakuma +10 more
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Isolation and Analytical Characterization of Local Malaysian Leech Saliva
International Islamic University Malaysia Engineering Journal, 2011 Leech saliva contains biologically active compounds that are mainly proteins and peptides. In this study a modified and smooth extraction method of saliva was used without leeches' scarification.
Mohamed Alaama +4 more
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Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis
Proteomes, 2016 The pioneering work by Patrick H. O’Farrell established two-dimensional gel electrophoresis as one of the most important high-resolution protein separation techniques of modern biochemistry (Journal of Biological Chemistry 1975, 250, 4007–4021).
Sandra Murphy +2 more
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