Results 11 to 20 of about 3,475,365 (278)

Gel Electrophoresis of Proteins [PDF]

open access: yes, 2012
Gel electrophoresis is a widely known group of techniques used to separate and identify macromolecules as DNA, RNA, or proteins based on size, form, or isoelectric point. The separation of molecules by electrophoresis is based on the fact that charged molecules migrate through a gel matrix upon application of an electric field.
Alberto Alcázar   +4 more
core   +6 more sources

Protein purification by Off-Gel electrophoresis [PDF]

open access: yesPROTEOMICS, 2002
A novel free-flow protein purification technique based on isoelectric electrophoresis is presented, where the proteins are purified in solution without the need of carrier ampholytes. The gist of the method is to flow protein solutions under an immobilised pH gradient gel (IPG) through which an electric field is applied perpendicular to the direction ...
Ros, A.   +8 more
openaire   +5 more sources

Discontinuous native protein gel electrophoresis [PDF]

open access: yesELECTROPHORESIS, 2006
AbstractAnalysis of the oligomeric state of a native protein usually requires analytical ultracentrifugation or repeated gel filtration to calculate the protein's size. We have developed a discontinuous native protein gel electrophoresis system that allows the separation of even basic proteins according to their size, oligomeric state, and shape.
Michael Niepmann, Junfeng Zheng
openaire   +3 more sources

Acute-phase protein concentration in bronchoalveolar lavage fluid from healthy horses [PDF]

open access: yesPesquisa Veterinária Brasileira, 2021
: Bronchoalveolar lavage fluid (BALF) was analyzed to obtain information on leakage of acute-phase proteins from the blood into the respiratory lumen and about local synthesis.
Paula Alessandra Di Filippo   +4 more
doaj   +1 more source

Identification of Structural Proteins of Rhizobium meliloti Temperate Phage 16-3 [PDF]

open access: yes, 1982
The structural proteins of Rhizobium meliloti temperate phage 16-3 have been analysed by means of polyacrylamide gel electrophoresis, isoelectric focusing and agarose gel electrophoresis.
Duda, Ernő   +3 more
core   +1 more source

ELECTROPHORETIC SYSTEMS FOR PREPARATIVE FRACTIONATION OF PROTEIN PRECURSORS OF BIOACTIVE PEPTIDES FROM COW’S MILK

open access: yesHarčova Nauka ì Tehnologìâ, 2018
The article considers the possibility of obtaining purified fractions-precursors of bioactive peptides from milk proteins by the method of preparative electrophoresis.
V. Yukalo   +3 more
doaj   +1 more source

Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) [PDF]

open access: yes, 2008
QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point.
Bernd Kastenholz
core   +2 more sources

Polyacrylamide gel electrophoresis of soybean seed proteins

open access: yesActa Societatis Botanicorum Poloniae, 2015
Four major and 14 minor protein bands were detected when total salt soluble proteins of soybean (Glycine max cultivar Warszawska) seed were subjected to polyacrylamide gel electrophoresis under nondissociating conditions, and 16 protein bands were ...
W. Lassocińsk, J. S. Knypl
doaj   +1 more source

An Improved Method for PAGE-based Detection of Phosphorylated Protein in Yeast

open access: yesBio-Protocol, 2012
One dimensional polyacrylamide gel electrophoresis has been successfully used to detect protein phosphorylation. This method is very simple and highly reproducible.
Yuehua Wei
doaj   +1 more source

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