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Capillary Sodium Dodecyl Sulfate Agarose Gel Electrophoresis of Proteins [PDF]

open access: yesGels, 2022
Capillary sodium dodecyl sulfate gel electrophoresis has long been used for the analysis of proteins, mostly either with entangled polymer networks or translationally cross-linked gels.
Daniel Sarkozy, Andras Guttman
doaj   +2 more sources

Gel Electrophoresis of Proteins [PDF]

open access: yes, 2012
Gel electrophoresis is a widely known group of techniques used to separate and identify macromolecules as DNA, RNA, or proteins based on size, form, or isoelectric point. The separation of molecules by electrophoresis is based on the fact that charged molecules migrate through a gel matrix upon application of an electric field.
Fernando Baquero   +4 more
openaire   +4 more sources

Polyacrylamide Gel Electrophoresis of Spinal-Fluid Proteins [PDF]

open access: bronzeClinical Chemistry, 1966
Abstract Twenty-three control samples of CSF were analyzed by vertical column electrophoresis on acrylamide gel. Quantitation results using a microdensitometer are presented. The normal protein pattern shows 15 constant bands which coincide in mobility and in many cases in staining pattern with serum proteins.
John H. Evans, Donald T. Quick
openaire   +3 more sources

Seed protein electrophoresis in plant genetics: Commemorating the pioneering contributions of Prof. Chittaranjan Kole and team to the foundation of plant proteomics [PDF]

open access: yesThe Plant Genome
Despite the rapid progress of proteomics in human and other model organisms, plant proteomics has advanced at a comparatively slower pace. This review aims to highlight the pioneering work on seed protein markers detected by employing gel electrophoresis
Sarita Pandey   +6 more
doaj   +2 more sources

Antigenic identity of immunodominant proteins of Bacillus anthracis genovariants

open access: yesЖурнал микробиологии, эпидемиологии и иммунобиологии, 2023
Introduction. The main biological raw materials for the production of immunobiological preparations for identification of Bacillus anthracis are its specific antigens, the protective antigen and the EA1 protein. Purpose.
Margarita P. Izhberdeeva   +3 more
doaj   +1 more source

Analysis on Gel Electrophoresis in Biology [PDF]

open access: yesE3S Web of Conferences, 2020
Gel electrophoresis is one of the basic methods used in modern biological laboratories to separate different compounds in a mixture. It is widely used in analyzing the amino acid sequence in proteins.
Cai Yiran
doaj   +1 more source

Protein purification by Off-Gel electrophoresis [PDF]

open access: yesPROTEOMICS, 2002
A novel free-flow protein purification technique based on isoelectric electrophoresis is presented, where the proteins are purified in solution without the need of carrier ampholytes. The gist of the method is to flow protein solutions under an immobilised pH gradient gel (IPG) through which an electric field is applied perpendicular to the direction ...
Ros, Alexandra   +8 more
openaire   +4 more sources

Discontinuous native protein gel electrophoresis [PDF]

open access: yesELECTROPHORESIS, 2006
AbstractAnalysis of the oligomeric state of a native protein usually requires analytical ultracentrifugation or repeated gel filtration to calculate the protein's size. We have developed a discontinuous native protein gel electrophoresis system that allows the separation of even basic proteins according to their size, oligomeric state, and shape.
Michael Niepmann, Junfeng Zheng
openaire   +3 more sources

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