Results 31 to 40 of about 862,871 (291)

Single-cell western blotting. [PDF]

, 2014
To measure cell-to-cell variation in protein-mediated functions, we developed an approach to conduct ∼10(3) concurrent single-cell western blots (scWesterns) in ∼4 h.
Herr, Amy E, Hughes, Alex J, Kang, Chi-Chih, Schaffer, David V, Spelke, Dawn P, Xu, Zhuchen   +5 more
core   +2 more sources

Polysaccharide as a Separation Medium for Gel Electrophoresis

Polysaccharides
Gel electrophoresis and size exclusion chromatography (SEC) are vital techniques in biochemical research, employing gel matrix structures made of polysaccharides or synthetic polymers like polyacrylamide for the analysis and separation of macromolecules.
Tsutomu Arakawa, Masataka Nakagawa, Chiaki Sakuma, Yui Tomioka, Yasunori Kurosawa, Teruo Akuta   +5 more
doaj   +1 more source

Efficacy of gel electrophoresis for proteins and biotechnological products –an overview.

Al-Mustansiriyah Journal of Pharmaceutical Sciences, 2022
Nucleic acids or proteins electrophoresed within a matrix or gel that immersed in a buffer provides ions needed to carry a current and for pH maintenance at a relatively constant value. Sodium dodecyl sulphate–polyacrylamide gel electrophoresis   (
Ammar Q. Muhammed, Nidhal K. Maraie, Basma T. Al-Sudani   +2 more
doaj   +1 more source

Detergents and Chaotropes for Protein Solubilization before Two-Dimensional Electrophoresis [PDF]

, 2006
Because of the outstanding separating capabilities of two-dimensional electrophoresis for complete proteins, it would be advantageous to be able to apply it to all types of proteins.
Chevallet, Mireille, Luche, Sylvie, Rabilloud, Thierry, Santoni, Véronique   +3 more
core   +4 more sources

Total Protein Extraction from Cultured Cells for Use in Electrophoresis and Western Blotting

BioTechniques, 1996
Experimentation with cultured cells often requires analyzing cellular protein extract by gel electrophoresis and immunoblotting. Traditional methods for extracting cellular proteins by homogenization or detergent solubilization usually produce protein ...
Kevin K.W. Wang, Avigail Posner, Iradj Hajimoharmmadreza   +2 more
doaj   +1 more source

Mitochondrial proteomics: analysis of a whole mitochondrial extract with two-dimensional electrophoresis [PDF]

, 2008
Mitochondria are complex organelles, and their proteomics analysis requires a combination of techniques. The emphasis in this chapter is made first on mitochondria preparation from cultured mammalian cells, then on the separation of the mitochondrial ...
A. Gorg, C. Tastet, D. Mokranjac, F. Gharahdaghi, F. Granier, H. Zischka, J. C. Sanchez, M. Chevallet, M. Eschenbruch, P. Lescuyer, S. Iwata, S. Luche, S. Luche, S. Richert, T. Rabilloud, T. Tsukihara, V. Santoni   +16 more
core   +6 more sources

Protein heterogeneity of lipoprotein particles containing apolipoprotein A-I without apolipoprotein A-II and apolipoprotein A-I with apolipoprotein A-II isolated from human plasma.

Journal of Lipid Research, 1988
The protein heterogeneity of fractions isolated by immunoaffinity chromatography on anti-apolipoprotein A-I and anti-apolipoprotein A-II affinity columns was analyzed by high resolution two-dimensional gel electrophoresis.
R W James, D Hochstrasser, J D Tissot, M Funk, R Appel, F Barja, C Pellegrini, A F Muller, D Pometta   +8 more
doaj   +1 more source

Membrane proteins and proteomics: Love is possible, but so difficult [PDF]

, 2009
Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with ...
Adessi, Aebersold, Aivaliotis, Aivaliotis, Alla, Ames, Babu, Bagshaw, Bell, Berlanda-Scorza, Berven, Bierczynska-Krzysik, Bisle, Bjellqvist, Bunai, Bunai, Burré, Buxbaum, Casey, Chevallet, Chevallet, Connolly, Coughenour, Cutillas, Devreese, Eravci, Everberg, Felsted, Ferro, Gatlin, Ghosh, Gyenes, Görg, Hagan, Hahne, Hartinger, Helling, Henningsen, Henzel, Hisabori, Hjelmeland, Huang, Huang, James, Jun, Lasonder, Lescuyer, Liberatori, Lopez, Low, Luche, Lyubimova, Lüddens, MacFarlane, MacFarlane, Matsudaira, McDonough, Miguet, Moebius, Molloy, Molloy, Nandakumar, Natera, Navarre, Ong, Papasotiriou, Pappin, Patton, Pedersen, Peng, Perdew, Phadke, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Rais, Rubin, Santoni, Santoni, Schindler, Schirle, Schägger, Siroy, Speers, Sweetnam, Switzer, Tastet, Taylor, Towbin, Veith, Washburn, Werner, Wilkins, Williams, Williams, Williams, Wittig, Witzmann, Wu, Yates, Ying, Zahedi   +103 more
core   +5 more sources

Polyacrylamide Gel Electrophoresis of Spinal-Fluid Proteins

Clinical Chemistry, 1966
Abstract Twenty-three control samples of CSF were analyzed by vertical column electrophoresis on acrylamide gel. Quantitation results using a microdensitometer are presented. The normal protein pattern shows 15 constant bands which coincide in mobility and in many cases in staining pattern with serum proteins.
J H, Evans, D T, Quick
openaire   +2 more sources

Identification of antigenic proteins from salivary glands of female Anopheles maculatus by proteomic analysis

Asian Pacific Journal of Tropical Biomedicine, 2016
Objective: To identify antigenic proteins from the salivary glands of female Anopheles maculatus using a proteomic approach to find the biomarker candidate for serological tools.
Yunita Armiyanti, Renam Putra Arifianto, Elisa Nurma Riana, Kartika Senjarini, Widodo Widodo, Loeki Enggar Fitri, Teguh Wahju Sardjono   +6 more
doaj   +1 more source