Results 31 to 40 of about 916,712 (391)

SDS capillary gel electrophoresis of proteins in microfabricated channels.

Proceedings of the National Academy of Sciences of the United States of America, 1999
Analysis of variations in the concentrations or structures of biomolecules (e.g., mRNAs, proteins, peptides, natural products) that occur either naturally or in response to environmental or genetic perturbations can provide important insight into complex
S. Yao, D. Anex, W. B. Caldwell, D. Arnold, Katherine B. Smith, P. Schultz   +5 more
semanticscholar   +1 more source

Application of Fluorescence Two-Dimensional Difference In-Gel Electrophoresis as a Proteomic Biomarker Discovery Tool in Muscular Dystrophy Research

Biology, 2013
In this article, we illustrate the application of difference in-gel electrophoresis for the proteomic analysis of dystrophic skeletal muscle. The mdx diaphragm was used as a tissue model of dystrophinopathy.
Steven Carberry, Margit Zweyer, Dieter Swandulla, Kay Ohlendieck   +3 more
doaj   +1 more source

Analysis of 953 human proteins from a mitochondrial HEK293 fraction by complexome profiling. [PDF]

PLoS ONE, 2013
Complexome profiling is a novel technique which uses shotgun proteomics to establish protein migration profiles from fractionated blue native electrophoresis gels.
Hans J C T Wessels, Rutger O Vogel, Robert N Lightowlers, Johannes N Spelbrink, Richard J Rodenburg, Lambert P van den Heuvel, Alain J van Gool, Jolein Gloerich, Jan A M Smeitink, Leo G Nijtmans   +9 more
doaj   +1 more source

Two-dimensional electrophoresis as a tool for structural and genetic studies of seed proteins from Poaceae and Fagaceae. [PDF]

, 1988
The application of two-dimensional electrophoretic procedures to structural and genetic studies of seed proteins from Poaceae (including the cultivated cereals) and Fagaceae is described.
Aragoncillo Ballesteros, Cipriano, Collada Collada, Maria Carmen, García Olmedo, Francisco, Gómez, Luis, Salcedo Duran, Gabriel, Sánchez-Monge Laguna de Rins, Rosa   +5 more
core   +2 more sources

Western Blot Analysis of Chloroplast HSP70B in Chlorella Species

Bio-Protocol, 2013
Western blotting allows for the specific detection of proteins by an antibody of interest. This protocol utilizes isolation of total proteins protocol for Chlorella vulgaris prior to gel electrophoresis. After electrophoresis, the selected antibodies are
Stephka Chankova, Zhana Mitrovska, Nadezhda Yurina   +2 more
doaj   +1 more source

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins of newbonr rat skin. II. Keratohyalin and stratum corneum proteins.

Journal of Investigative Dermatology, 1975
Keratohyalin extracts from newborn rat epidermis were prepared by potassium phosphate and citric acid-detergent extraction procedures. These preparations were compared by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and amino acid ...
B. A. Dale, Irving B. Stern
semanticscholar   +1 more source

Capabilities and limitations of gel electrophoresis for elemental speciation : a laboratory's experience [PDF]

, 2006
Gel electrophoresis is a fractionation/separation technique that yields valuable information in the field of metalloproteomics, often referred to as metallomics. This paper is based on four years of practical experience of the authors' lab in this domain
Chéry, Cyrille C, Cornelis, Rita, Moens, Luc, Vanhaecke, Frank   +3 more
core   +2 more sources

Polysaccharide as a Separation Medium for Gel Electrophoresis

Polysaccharides
Gel electrophoresis and size exclusion chromatography (SEC) are vital techniques in biochemical research, employing gel matrix structures made of polysaccharides or synthetic polymers like polyacrylamide for the analysis and separation of macromolecules.
Tsutomu Arakawa, Masataka Nakagawa, Chiaki Sakuma, Yui Tomioka, Yasunori Kurosawa, Teruo Akuta   +5 more
doaj   +1 more source

Total Protein Extraction from Cultured Cells for Use in Electrophoresis and Western Blotting

BioTechniques, 1996
Experimentation with cultured cells often requires analyzing cellular protein extract by gel electrophoresis and immunoblotting. Traditional methods for extracting cellular proteins by homogenization or detergent solubilization usually produce protein ...
Kevin K.W. Wang, Avigail Posner, Iradj Hajimoharmmadreza   +2 more
doaj   +1 more source

Efficacy of gel electrophoresis for proteins and biotechnological products –an overview.

Al-Mustansiriyah Journal of Pharmaceutical Sciences, 2022
Nucleic acids or proteins electrophoresed within a matrix or gel that immersed in a buffer provides ions needed to carry a current and for pH maintenance at a relatively constant value. Sodium dodecyl sulphate–polyacrylamide gel electrophoresis   (
Ammar Q. Muhammed, Nidhal K. Maraie, Basma T. Al-Sudani   +2 more
doaj   +1 more source