Results 31 to 40 of about 3,553,338 (335)

Western Blot Analysis of Chloroplast HSP70B in Chlorella Species

Bio-Protocol, 2013
Western blotting allows for the specific detection of proteins by an antibody of interest. This protocol utilizes isolation of total proteins protocol for Chlorella vulgaris prior to gel electrophoresis. After electrophoresis, the selected antibodies are
Stephka Chankova, Zhana Mitrovska, Nadezhda Yurina   +2 more
doaj   +1 more source

Analysis of 953 human proteins from a mitochondrial HEK293 fraction by complexome profiling. [PDF]

PLoS ONE, 2013
Complexome profiling is a novel technique which uses shotgun proteomics to establish protein migration profiles from fractionated blue native electrophoresis gels.
Hans J C T Wessels, Rutger O Vogel, Robert N Lightowlers, Johannes N Spelbrink, Richard J Rodenburg, Lambert P van den Heuvel, Alain J van Gool, Jolein Gloerich, Jan A M Smeitink, Leo G Nijtmans   +9 more
doaj   +1 more source

Nigrosine staining of wheat endosperm proteolipid patterns on starch gels [PDF]

, 1975
We have previously characterized a group of proteolipids from wheat endosperm, designated CM proteins, which are soluble in chloroformmethanol (2:1, v/v) and have a molecular weight lower than 25,000 daltons (1-3).
Aragoncillo, Boyd, C. Aragoncillo, Coulson, Elton, Ewart, F. Garcia-Olmedo, Feillet, Garcia Olmedo, Garcia Olmedo, Graham, Jennings, M.A. Rodriguez-Loperena, Mita, Pilar Carbonero, Redman, Solari, Woychick, Wrigley, Yong   +19 more
core   +2 more sources

Two-dimensional gel electrophoresis in proteomics: past, present and future [PDF]

Journal of proteomics (2010) epub ahead of print, 2010
Two-dimensional gel electrophoresis has been instrumental in the birth and developments of proteomics, although it is no longer the exclusive separation tool used in the field of proteomics. In this review, a historical perspective is made, starting from the days where two-dimensional gels were used and the word proteomics did not even exist.
arxiv   +1 more source

Polysaccharide as a Separation Medium for Gel Electrophoresis

Polysaccharides
Gel electrophoresis and size exclusion chromatography (SEC) are vital techniques in biochemical research, employing gel matrix structures made of polysaccharides or synthetic polymers like polyacrylamide for the analysis and separation of macromolecules.
Tsutomu Arakawa, Masataka Nakagawa, Chiaki Sakuma, Yui Tomioka, Yasunori Kurosawa, Teruo Akuta   +5 more
doaj   +1 more source

Total Protein Extraction from Cultured Cells for Use in Electrophoresis and Western Blotting

BioTechniques, 1996
Experimentation with cultured cells often requires analyzing cellular protein extract by gel electrophoresis and immunoblotting. Traditional methods for extracting cellular proteins by homogenization or detergent solubilization usually produce protein ...
Kevin K.W. Wang, Avigail Posner, Iradj Hajimoharmmadreza   +2 more
doaj   +1 more source

Efficacy of gel electrophoresis for proteins and biotechnological products –an overview.

Al-Mustansiriyah Journal of Pharmaceutical Sciences, 2022
Nucleic acids or proteins electrophoresed within a matrix or gel that immersed in a buffer provides ions needed to carry a current and for pH maintenance at a relatively constant value. Sodium dodecyl sulphate–polyacrylamide gel electrophoresis   (
Ammar Q. Muhammed, Nidhal K. Maraie, Basma T. Al-Sudani   +2 more
doaj   +1 more source

Capabilities and limitations of gel electrophoresis for elemental speciation : a laboratory's experience [PDF]

, 2006
Gel electrophoresis is a fractionation/separation technique that yields valuable information in the field of metalloproteomics, often referred to as metallomics. This paper is based on four years of practical experience of the authors' lab in this domain
Chéry, Cyrille C, Cornelis, Rita, Moens, Luc, Vanhaecke, Frank   +3 more
core   +2 more sources

Development of Pipetting Devices to Separate Protein Complexes [PDF]

arXiv, 2022
The objective of this project is to develop an automated device used to spot protein samples on a hydrophobic membrane to be used for the patented electrophoresis method developed by Chang and Yonan in 2008 [1]. This novel method performs electrophoresis directly on hydrophobic blot membranes as opposed to the previous popular methods such as the 2-D ...
arxiv  

The Whereabouts of 2D Gels in Quantitative Proteomics [PDF]

Methods in Molecular Biology -Clifton then Totowa- 893 (2012) 25-35, 2012
Two-dimensional gel electrophoresis has been instrumental in the development of proteomics. Although it is no longer the exclusive scheme used for proteomics, its unique features make it a still highly valuable tool, especially when multiple quantitative comparisons of samples must be made, and even for large samples series.
arxiv   +1 more source