Results 31 to 40 of about 3,475,365 (278)
Carbohydrate Affinity PAGE for the Study of Carbohydrate-Binding Proteins
BioTechniques, 1998 Immobilized neoglycoconjugates covalently cross-linked into a polyacrylamide gel can be used to detect and characterize carbohydrate-binding proteins.
Phil D. Rye, Nicolai V. Bovin
doaj +1 more source
Antibodies, 2022 In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the ...
Teruo Akuta +11 more
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Study of protein expresion [sic] in peri-infarct tissue after cerebral ischemia [PDF]
, 2015 In this work, we report our study of protein expression in rat peri-infarct tissue, 48 h after the induction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis with mass spectrometry and combined fractional diagonal ...
Agulla, Jesús +9 more
core +5 more sources
False-Negative Urine Protein Electrophoresis by Semiautomated Gel Electrophoresis [PDF]
Clinical Chemistry, 2004 Urine is evaluated by electrophoretic methods both to gather information about the location and degree of damage within the nephron and to detect and quantify monoclonal free light chains (Bence Jones proteins). For many years, urine protein electrophoresis consisted of concentrating the urine (usually >50-fold) followed by manual application of the ...
Stephen J. Ebrom +2 more
openaire +3 more sources
A two-dimensional electrophoresis protocol suitable for Medicago truncatula leaf proteome [PDF]
, 2015 Medicago truncatula leaves were used as the experimental materials. Total proteins of leaves were extracted by trichloracetic acid (TCA)-acetone method and proteins had a better separation using gel strips, forming an immobilized non-linear 3 to 10 pH ...
Dong, Wen +7 more
core +2 more sources
Variations on a theme: Changes to electrophoretic separations that can make a difference [PDF]
Journal of proteomics (2010) epub ahead of print, 2010 Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
arxiv +1 more source
Biology, 2013 In this article, we illustrate the application of difference in-gel electrophoresis for the proteomic analysis of dystrophic skeletal muscle. The mdx diaphragm was used as a tissue model of dystrophinopathy.
Steven Carberry +3 more
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Analysis of 953 human proteins from a mitochondrial HEK293 fraction by complexome profiling. [PDF]
PLoS ONE, 2013 Complexome profiling is a novel technique which uses shotgun proteomics to establish protein migration profiles from fractionated blue native electrophoresis gels.
Hans J C T Wessels +9 more
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Western Blot Analysis of Chloroplast HSP70B in Chlorella Species
Bio-Protocol, 2013 Western blotting allows for the specific detection of proteins by an antibody of interest. This protocol utilizes isolation of total proteins protocol for Chlorella vulgaris prior to gel electrophoresis. After electrophoresis, the selected antibodies are
Stephka Chankova +2 more
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Two-dimensional gel electrophoresis in proteomics: past, present and future [PDF]
Journal of proteomics (2010) epub ahead of print, 2010 Two-dimensional gel electrophoresis has been instrumental in the birth and developments of proteomics, although it is no longer the exclusive separation tool used in the field of proteomics. In this review, a historical perspective is made, starting from the days where two-dimensional gels were used and the word proteomics did not even exist.
arxiv +1 more source