Results 41 to 50 of about 929,641 (371)

False-Negative Urine Protein Electrophoresis by Semiautomated Gel Electrophoresis [PDF]

Clinical Chemistry, 2004
Urine is evaluated by electrophoretic methods both to gather information about the location and degree of damage within the nephron and to detect and quantify monoclonal free light chains (Bence Jones proteins). For many years, urine protein electrophoresis consisted of concentrating the urine (usually >50-fold) followed by manual application of the ...
Stephen J. Ebrom, David F. Keren, Ronald Gulbranson   +2 more
openaire   +3 more sources

Single-cell western blotting. [PDF]

, 2014
To measure cell-to-cell variation in protein-mediated functions, we developed an approach to conduct ∼10(3) concurrent single-cell western blots (scWesterns) in ∼4 h.
Herr, Amy E, Hughes, Alex J, Kang, Chi-Chih, Schaffer, David V, Spelke, Dawn P, Xu, Zhuchen   +5 more
core   +2 more sources

Application of Fluorescence Two-Dimensional Difference In-Gel Electrophoresis as a Proteomic Biomarker Discovery Tool in Muscular Dystrophy Research

Biology, 2013
In this article, we illustrate the application of difference in-gel electrophoresis for the proteomic analysis of dystrophic skeletal muscle. The mdx diaphragm was used as a tissue model of dystrophinopathy.
Steven Carberry, Margit Zweyer, Dieter Swandulla, Kay Ohlendieck   +3 more
doaj   +1 more source

A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting

Antibodies, 2022
In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the ...
Teruo Akuta, Toshiaki Maruyama, Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Kevin Entzminger, Jonathan K. Fleming, Ryo Sato, Takashi Shibata, Yasunori Kurosawa, C. J. Okumura, Tsutomu Arakawa   +11 more
doaj   +1 more source

Agarose gel serum protein electrophoresis in cats with and without lymphoma and preliminary results of tandem mass fingerprinting analysis [PDF]

, 2011
<b>Background</b>: Serum electrophoretic profiles in cats are poorly characterized with respect to the protein components of the globulin fractions, and interpretation of the electrophoretograms has routinely been done in ignorance of the ...
Baker, Bence, Bing, Ceron, Cerón, Choi-Miura, Correa, Corzo, Dust, Eckersall, Furukawa, Giordano, González-Ramón, Hahn, Hofmann-Lehmann, Jeppsson, Keay, Kristensen, Lampreave, Laurell, MacEwen, McGrotty, Merlo, Moore, Nielsen, Ogilvie, Paltrinieri, Paltrinieri, Prenni, Roman, Saguchi, Selting, Sparkes, Stockham, Vail, Vavricka, Wiechselbaum   +36 more
core   +1 more source

Western Blot Analysis of Chloroplast HSP70B in Chlorella Species

Bio-Protocol, 2013
Western blotting allows for the specific detection of proteins by an antibody of interest. This protocol utilizes isolation of total proteins protocol for Chlorella vulgaris prior to gel electrophoresis. After electrophoresis, the selected antibodies are
Stephka Chankova, Zhana Mitrovska, Nadezhda Yurina   +2 more
doaj   +1 more source

Analysis of 953 human proteins from a mitochondrial HEK293 fraction by complexome profiling. [PDF]

PLoS ONE, 2013
Complexome profiling is a novel technique which uses shotgun proteomics to establish protein migration profiles from fractionated blue native electrophoresis gels.
Hans J C T Wessels, Rutger O Vogel, Robert N Lightowlers, Johannes N Spelbrink, Richard J Rodenburg, Lambert P van den Heuvel, Alain J van Gool, Jolein Gloerich, Jan A M Smeitink, Leo G Nijtmans   +9 more
doaj   +1 more source

Analysis of proteins from marine molluscs [PDF]

, 2009
Application of the two-dimensional gel electrophoresis (2DE) protocols which were developed for samples of mammalian origin gives unsatisfactory results when used in samples from marine molluscs. This chapter describes a detailed protocol of 2DE that can
Bebianno, Maria João, Chora, Suze, Roméo, Michèle   +2 more
core   +1 more source

Polysaccharide as a Separation Medium for Gel Electrophoresis

Polysaccharides
Gel electrophoresis and size exclusion chromatography (SEC) are vital techniques in biochemical research, employing gel matrix structures made of polysaccharides or synthetic polymers like polyacrylamide for the analysis and separation of macromolecules.
Tsutomu Arakawa, Masataka Nakagawa, Chiaki Sakuma, Yui Tomioka, Yasunori Kurosawa, Teruo Akuta   +5 more
doaj   +1 more source

Total Protein Extraction from Cultured Cells for Use in Electrophoresis and Western Blotting

BioTechniques, 1996
Experimentation with cultured cells often requires analyzing cellular protein extract by gel electrophoresis and immunoblotting. Traditional methods for extracting cellular proteins by homogenization or detergent solubilization usually produce protein ...
Kevin K.W. Wang, Avigail Posner, Iradj Hajimoharmmadreza   +2 more
doaj   +1 more source