Polysaccharide as a Separation Medium for Gel Electrophoresis
PolysaccharidesGel electrophoresis and size exclusion chromatography (SEC) are vital techniques in biochemical research, employing gel matrix structures made of polysaccharides or synthetic polymers like polyacrylamide for the analysis and separation of macromolecules.
Tsutomu Arakawa +5 more
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Total Protein Extraction from Cultured Cells for Use in Electrophoresis and Western Blotting
BioTechniques, 1996 Experimentation with cultured cells often requires analyzing cellular protein extract by gel electrophoresis and immunoblotting. Traditional methods for extracting cellular proteins by homogenization or detergent solubilization usually produce protein ...
Kevin K.W. Wang +2 more
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Efficacy of gel electrophoresis for proteins and biotechnological products –an overview.
Al-Mustansiriyah Journal of Pharmaceutical Sciences, 2022 Nucleic acids or proteins electrophoresed within a matrix or gel that immersed in a buffer provides ions needed to carry a current and for pH maintenance at a relatively constant value. Sodium dodecyl sulphate–polyacrylamide gel electrophoresis (
Ammar Q. Muhammed +2 more
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Mapping cells and sub-cellular organelles on 2-D gels: ‘new tricks for an old horse’ [PDF]
, 1995 Nowadays, investigators in all fields are faced with the identification of unknown, up- or down-regulated, modified proteins that they are trying to identify.
Dahlback, B. +5 more
core +3 more sources
Development of Pipetting Devices to Separate Protein Complexes [PDF]
arXiv, 2022 The objective of this project is to develop an automated device used to spot protein samples on a hydrophobic membrane to be used for the patented electrophoresis method developed by Chang and Yonan in 2008 [1]. This novel method performs electrophoresis directly on hydrophobic blot membranes as opposed to the previous popular methods such as the 2-D ...
arxiv
Nigrosine staining of wheat endosperm proteolipid patterns on starch gels [PDF]
, 1975 We have previously characterized a group of proteolipids from wheat endosperm, designated CM proteins, which are soluble in chloroformmethanol (2:1, v/v) and have a molecular weight lower than 25,000 daltons (1-3).
Aragoncillo +19 more
core +2 more sources
The Whereabouts of 2D Gels in Quantitative Proteomics [PDF]
Methods in Molecular Biology -Clifton then Totowa- 893 (2012)
25-35, 2012 Two-dimensional gel electrophoresis has been instrumental in the development of proteomics. Although it is no longer the exclusive scheme used for proteomics, its unique features make it a still highly valuable tool, especially when multiple quantitative comparisons of samples must be made, and even for large samples series.
arxiv +1 more source
Two-dimensional electrophoresis as a tool for structural and genetic studies of seed proteins from Poaceae and Fagaceae. [PDF]
, 1988 The application of two-dimensional electrophoretic procedures to structural and genetic studies of seed proteins from Poaceae (including the cultivated cereals) and Fagaceae is described.
Aragoncillo Ballesteros, Cipriano +5 more
core +2 more sources
About thiol derivatization and resolution of basic proteins in two-dimensional electrophoresis [PDF]
Proteomics 4 (03/2004) 551-61, 2006 The influence of thiol blocking on the resolution of basic proteins by two-dimensional electrophoresis was investigated. Cysteine blocking greatly increased resolution and decreased streaking, especially in the basic region of the gels. Two strategies for cysteine blocking were found to be efficient: classical alkylation with maleimide derivatives and ...
arxiv +1 more source
Highlights on the capacities of "Gel-based" proteomics
Proteome Science, 2010 Gel-based proteomic is the most popular and versatile method of global protein separation and quantification. This is a mature approach to screen the protein expression at the large scale, and a cheaper approach as compared with gel-free proteomics ...
Chevalier François
doaj +1 more source