Results 101 to 110 of about 883,230 (298)

Transformation of phosphiothrici resistance gene(bar)in Undaria pinnatifida gametophytes [PDF]

open access: yes, 2012
裙带菜(Undaria pinnatifida)配子体经2.5 L气升式光生物反应器快速营养增殖,通过基因枪法将携带草丁膦抗性基因(bar)的载体转入扩增后的配子体,经草丁膦筛选,获得抗性克隆。提取抗性克隆配子体基因组总DNA,经PCR和PCR Southern检测,结果显示60%的抗性克隆可检出bar基因,表明bar基因是裙带菜配子体基因工程有效的筛选标记 ...
任宝永   +4 more
core  

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

open access: yesFEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson   +6 more
wiley   +1 more source

Assessment the Response of Chickpea Genotypes to Agrobacterium -Mediated Transformation System

open access: yesScience Journal of University of Zakho, 2016
Transformation protocol based on the inoculation of chickpea mature embryos with Agrobacterium suspension was carried out. Four chickpea lines and one Iraqi local variety were used as recipient to the foreign gene of Agrobacterium tumefaciens strain ...
Diaa A. Ibrahim   +2 more
doaj  

The Predicted Structure of S. cerevisiae Ssp1 Reveals Parallel Evolution in the Pil1 BAR Domain Family Proteins of Ascomycetes

open access: yesJournal of Fungi
BAR domains are a superfamily of widely conserved membrane binding motifs. In fungi, Pil1 family proteins are BAR domain containing proteins involved in organizing the plasma membrane. S.
Yasuyuki Suda, Aaron M. Neiman
doaj   +1 more source

Induction of competent cells for Agrobacterium tumefaciens-mediated stable transformation of common bean (Phaseolus vulgaris L.).

open access: yesPLoS ONE, 2020
Stable transformation of common bean (Phaseolus vulgaris L.) has been successful, to date, only using biolistic-mediated transformation and shoot regeneration from meristem-containing embryo axes. In this study, using precultured embryo axes, and optimal
Guo-Qing Song   +5 more
doaj   +1 more source

Expression and Inheritance of bar Gene in Petunia hybrida Transformed with Agrobacterium [PDF]

open access: yesJournal of Plant Biotechnology, 2003
This experiment was carried out to confirm the stability of bar gene introduced into petunia plant through Agrobacerium-mediated transformation. Twenty-five transgenic plants T plants, back cross (BC) populations to wild type and Fplants between different T plants were prepared, and polymerase chain reaction(PCR), PCR-Southern blot analysis, and field ...
openaire   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

open access: yesFEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa   +2 more
wiley   +1 more source

Mean field analysis of a spatial stochastic model of a gene regulatory network [PDF]

open access: yes, 2015
A gene regulatory network may be defined as a collection of DNA segments which interact with each other indirectly through their RNA and protein products.
Murray, P. J.   +3 more
core   +1 more source

18S rDNA sequencing data of benthic polychaetes from the Eastern Arabian Sea

open access: yesData in Brief, 2018
The limited DNA sequence data of the polychaetes species are available from the Eastern Arabian Sea. We have sequenced 18S rDNA gene from 54 polychaetes species and 37 species identified up to the species level.
Periasamy Rengaiyan, Baban Ingole
doaj   +1 more source

Coding variants identified in patients with diabetes alter PICK1 BAR domain function in insulin granule biogenesis

open access: yesThe Journal of Clinical Investigation, 2022
Bin/amphiphysin/Rvs (BAR) domains are positively charged crescent-shaped modules that mediate curvature of negatively charged lipid membranes during remodeling processes.
Rita C. Andersen   +21 more
doaj   +1 more source

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