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Efficient Sugarcane Transformation via bar Gene Selection
Tropical Plant Biology, 2017Genetic engineering provides new opportunities for improving economically important traits in sugarcane cultivars. In this study, an efficient Agrobacterium-mediated transformation system that uses the bar gene (a herbicide resistance gene that is used in conjunction with the herbicide Basta) as a selection marker was developed.
W. Z. Wang +6 more
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Effect of the Barring Gene on Eye Pigmentation in the Fowl
Pigment Cell Research, 1989Pigment cells of the iris, pecten, retinal pigment epithelium, and choroid of the wild‐type jungle fowl (JF) and the barred Plymouth rock (BPR) breeds of adult chickens were studied at both light and electron microscopic levels. BPR choroidal tissues had 2.8 times fewer melanophores than the JF choroid, and BPR melanophores also contained 2.4 times ...
R E, Schreck, R R, Bowers
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Early clinical data raise the bar for hemophilia gene therapies
Nature Biotechnology, 2016exaly +3 more sources
The bar Gene as Selectable and Screenable Marker in Plant Engineering
1992Publisher Summary This chapter explains the use of the bar gene as a selectable marker in plant transformation, as a screenable marker in tissue culture and plant breeding, and as a reporter gene in plant molecular biology. The bar gene has served as a useful assayable marker gene in plant molecular biology.
K, D'Halluin +6 more
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Transformation of oat and inheritance of bar gene expression
Plant Cell, Tissue and Organ Culture, 2001Fertile transgenic plants of oat (Avena sativa L. var. Melys) were produced following microprojectile bombardment of primary embryogenic calli from immature embryos with two plasmids containing the bar gene or the β-glucuronidase (uidA) gene, after selection with glufosinate ammonium.
B. Kuai +5 more
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Distinct expression patterns for two Xenopus Bar homeobox genes
Development Genes and Evolution, 2000The BarH1 and BarH2 homeobox genes are coexpressed in cells of the fly retina and in the central and peripheral nervous systems. The fly Bar genes are required for normal development of the eye and external sensory organs. In Xenopus we have identified two distinct vertebrate Bar-related homeobox genes, XBH1 and XBH2. XBH1 is highly related in sequence
K D, Patterson +4 more
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Production of herbicide-resistant sweet potato plants transformed with the bar gene
Biotechnology Letters, 2007Herbicide-resistant sweet potato plants were produced through biolistics of embryogenic calli derived from shoot apical meristems. Plant materials were bombarded with the vectors containing the beta-glucuronidase gene (gusA) and the herbicide-resistant gene (bar). Selection was carried out using phosphinothricin (PPT).
Gibum, Yi +5 more
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[Transformation of bar gene to tetraploid of Isatis indigotica].
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, 2004The transgenic tetraploid of Isatis indigotica mediated by Agrobacterium tumefaciens was obtained. To transfer the plant binary expression vector pCAMBIA 3300 carrying bar gene, the Agrobacterium tumefaciens strain EHA 105 was used as engineering bacterium.
Tiefeng, Xu +5 more
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Stable expression of the promoterless bar gene in transformed rapeseed plants
Cytology and Genetics, 2008Phosphinothricin-resistant plants of commercial summer rapeseed varieties (Brassica napus L. var. oleifera DC) were obtained by means of Agrobacterium tumefaciens-mediated transformation of leaf disks. The vector structures contained the promoterless coding sequence of the phosphinothricin acetyltransferase gene (bar), which is situated between two ...
L. A. Sakhno +3 more
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Patent Office May Raise the Bar on Gene Claims
Science, 2000BIOMEDICAL PATENTSIn a major shift, the U.S. Patent and Trademark Office (PTO) has proposed a policy that will raise the bar for patent applications on DNA--a change that could lead to the rejection of many claims that have been stalled because of enduring questions over exactly what can be patented.
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