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Plant Trait Gene Expression Cassette Design
2015Plant biotechnology depends on robust control of trait gene expression to produce an adequate level of protein or RNA in the appropriate cells at the correct time. Early biotechnology products depended on high protein production in most plant cells, and relied on relatively few well-characterized “promoters.” This demand continues and has expanded into
Michael Nuccio +4 more
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MX Cassettes for Knocking Out Genes in Yeast
Cold Spring Harbor Protocols, 2017Precise modifications of the Saccharomyces cerevisiae genome use marker cassettes, most often in the form of “knockout” (KO) marker cassettes, to delete genes. Many different KO marker cassettes exist, some of which require strains with specific genotypes, such as auxotrophic mutations, and others that have no strain genotype requirements, such as ...
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Ecology of the integron gene cassette metagenome
2022Integrons are genetic elements that promote rapid adaptation in bacteria by capturing exogenous mobile gene cassettes. Recently, a sub-set of gene cassettes have facilitated the global spread of antibiotic resistance, however, outside of clinical settings, very little is known about the function and ecology of these cassettes.
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Design of Muscle Gene Therapy Expression Cassette
2019The first gene therapy drug approved by the European Regulatory Commission involves the transfer of a therapeutic gene to the muscle by adeno-associated viral vector (AAV). Now, muscle gene transfer is quickly becoming a therapy of choice for muscle and non-muscle diseases.
Yi Lai, Dongsheng Duan
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Integrative oncology: Addressing the global challenges of cancer prevention and treatment
Ca-A Cancer Journal for Clinicians, 2022Jun J Mao,, Msce +2 more
exaly
β-Globin expression cassette 2.0: Optimizing gene therapy cassettes using RMCE
Blood Cells, Molecules, and Diseases, 2007Romain Desprat, Eric E. Bouhassira
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Construction disruption cassettes for gene silencing
Clinical Biochemistry, 2011Fateme Nafian Dehkordi, Somaye Mazaheri
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[Mobile gene cassettes and DNA integration elements ].
Molekuliarnaia biologiia, 2002Integrons are unique natural systems for capturing and spreading the antibiotic resistance genes among Gram-negative bacteria. Gene transfer into small genomes and into plasmids is via site-specific recombination. Integrons act as receptors of antibiotic resistance cassettes. There are known more than 50 cassettes conferring resistance to beta-lactams,
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Random Chromosomal Gene Disruption Using Cassette Mutagenesis
2002French A, Lewis, Brian A, Dougherty
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