Results 31 to 40 of about 835,345 (308)

Demonstration of the Presence of the "Deleted" MIR122 Gene in HepG2 Cells [PDF]

, 2015
MicroRNA 122 (miR-122) is highly expressed in the liver where it influences diverse biological processes and pathways, including hepatitis C virus replication and metabolism of iron and cholesterol.
Fei, Y, Hamad, IA, Humphries, SE, Kalea, AZ, Palmen, J, Smith, AJ, Talmud, PJ, Walker, AP, Yin, D   +8 more
core   +1 more source

Impact of air pollution and heavy metal exposure on sperm quality: A clinical prospective research study

Toxicology Reports
Exposure to air pollution poses significant risks to human health, including detrimental effects on the reproductive system, affecting both men and women.
Abilash D, Sridharan T B
doaj   +1 more source

Macrophage migration inhibitory factor (MIF) family in arthropods : Cloning and expression analysis of two MIF and one D-dopachrome tautomerase (DDT) homologues in Mud crabs, Scylla paramamosain [PDF]

, 2016
Acknowledgements This research was supported by grants from the National Natural Science Foundation of China (Nos. 31172438 and U1205123), the Natural Science Foundation of Fujian Province (No.
Duan, Li-Peng, Huang, Bei, Huang, Wen-Shu, Jia, Qin-qin, Nie, Pin, Wang, Ke-Jian, Wang, Tiehui, Zhang, Cai-Liang   +7 more
core   +1 more source

Genomic Organization, Splice Variants and Expression of CGMl, a CD66-related Member of the Carcinoembryonic Antigen Gene Family [PDF]

, 1993
The tumor marker carcinoembryonic antigen (CEA) belongs to a family of proteins which are composed of one immunogiobulin variable domain and a varying number of immunoglobulin constant-like domains.
Afar D. E. H., Audette M., Baniyash M., Berling B., Culic O., Elsen P., Felgner P. L., Fiddes J. C., Knapp W., Kolbinger F., Kuroki M., Kuroki M., Kuroki M., Lin S.-H., Lucas K., Margolis R. N., McCuaig K., Nap M., Neumaier M., Ng S. Y., Oikawa S., Oikawa S., Paxton R., Pelegrin A., Raskind W. H., Rojas M., Sambrook J. E., Schwarz K., Shively J. E., Skubitz K. M., Southern P. J., Thompson J. A., Thompson J. A., Turbide C., Tynan K., Watt S. M., Woodgett J. R., Zhou H.   +37 more
core   +7 more sources

Standardized cloning vectors for protein production and generation of large gene libraries in Escherichia coli

BioTechniques, 2018
Here, we modified the multiple cloning sites from commonly used expression vectors to create a new suite of cloning plasmids that simplify and speed up cloning procedures in Escherichia coli.
Bettina Rohweder, Florian Semmelmann, Christiane Endres, Reinhard Sterner   +3 more
doaj   +1 more source

Streamlined assembly of cloning and genome editing vectors for genus Clostridium

iScience, 2023
Summary: Reported herein is a new set of vectors designed to streamline molecular cloning and genome editing by exploiting modern cloning methods. The new vectors build on the existing pMTL8000 vectors that have been a staple of Clostridium research for ...
Tom S. Bailey, Philip Hittmeyer, Yanchao Zhang, Aleksandra M. Kubiak   +3 more
doaj   +1 more source

Accelerated Evolution of the ASPM Gene Controlling Brain Size Begins Prior to Human Brain Expansion [PDF]

, 2004
Primary microcephaly (MCPH) is a neurodevelopmental disorder characterized by global reduction in cerebral cortical volume. The microcephalic brain has a volume comparable to that of early hominids, raising the possibility that some MCPH genes may have ...
Abeysinghe, Aota, Benson, Bernardi, Bond, Bond, Chen, Chenn, Chuzhanova, Clark, Crandall, Dewyse, do Carmo Avides, Duret, Endo, Evans, Galtier, Gould, Hughes, Jurka, Kent, Kouprina, Kreitman, McCreary, McDonald, Mochida, Morgenstern, Noskov, Polushin, Rehen, Riparbelli, Ripoll, Roberts, Rudiger, Sharp, Sharp, Tobias, Wood, Yang, Yang, Zhang   +40 more
core   +3 more sources

Creation and validation of a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells

BMC Biotechnology, 2012
Background Cloning vectors capable of retroviral transduction have enabled stable gene overexpression in numerous mitotic cell lines. However, the relatively small number of feasible restriction enzyme sequences in their cloning sites can hinder ...
Patel Asmita, Muñoz Anisleidys, Halvorsen Katherine, Rai Priyamvada   +3 more
doaj   +1 more source

By dawn or dusk—how circadian timing rewrites bacterial infection outcomes

FEBS Letters, EarlyView.
The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo, Catherine S. Palmer, Jacqueline M. Kimmey   +2 more
wiley   +1 more source

TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression. [PDF]

PLoS ONE, 2017
During the last few decades, the recombinant protein expression finds more and more applications. The cloning of protein-coding genes into expression vectors is required to be directional for proper expression, and versatile in order to facilitate gene ...
Athanasios Niarchos, Anastasia Siora, Evangelia Konstantinou, Vasiliki Kalampoki, George Lagoumintzis, Konstantinos Poulas   +5 more
doaj   +1 more source