Results 61 to 70 of about 835,345 (308)

T-DNA promoter tagging in Nicotiana tabacum : a thesis presented in fulfilment of the requirements for the degree of Master of Philosophy in Genetics at Massey University, Palmerston North, New Zealand [PDF]

, 1997
Plant development is primarily controlled at the level of gene expression. In order to analyse this regulation it is necessary to isolate genes which are involved in organ development through cellular and tissue determination or which respond to ...
Kerr, Donald Haldane
core  

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Molecular cloning and transcriptional activity of a new Petunia calreticulin gene involved in pistil transmitting tract maturation, progamic phase, and double fertilization [PDF]

, 2013
Calreticulin (CRT) is a highly conserved and ubiquitously expressed Ca2+-binding protein in multicellular eukaryotes. As an endoplasmic reticulum-resident protein, CRT plays a key role in many cellular processes including Ca2+ storage and release ...
A Christensen, AJ Crofts, AM Hassan, Anna Suwińska, BA Kwiatkowski, BQ Huang, CM Williams, CN Nardi, CO Lim, DE Nelson, E Gasteiger, E Zuckerkandl, F Chen, H Jin, HJ Li, HJ Li, HQ Tian, J Denecke, J Felsenstein, J Li, JC Huang, JL Went Van, JR Nevins, Justyna Prusińska, K Hsieh, K Tamura, Krzysztof Gumowski, L Navazio, L Thelin, LI Gold, LL Ge, M Iwano, M Lenartowska, M Lenartowska, M Lenartowska, M Michalak, MA Larkin, Marta Lenartowska, MTM Willemse, N Blom, N Borisjuk, N Saitou, P Mariani, P Shaw, Robert Lenartowski, S Persson, S Persson, SF Altschul, SJ Coughlan, T Dresselhaus, T Ishimizu, UK Tirlapur, WA Wang, XL Ye, XY Jia, XY Jia, Y Fu, Y Saijo, YQ An, Z Li   +59 more
core   +2 more sources

β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1

Molecular Oncology, EarlyView.
Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero, Alejandro Belmonte‐Fernández, Mónica González‐Moreno, Laura Rodríguez‐Cordero, Begoña Pérez‐Valderrama, Joaquín Herrero‐Ruíz, Francisco Romero, Carmen Sáez, Miguel Á. Japón   +8 more
wiley   +1 more source

Mutagenesis and Gene Cloning in Schizosaccharomyces pombe Using Nonhomologous Plasmid Integration and Rescue

BioTechniques, 2000
Genes are commonly cloned in yeasts and bacteria by plasmid complementation, where the introduction of the gene of interest into a host strain carrying a recessive mutation in that gene suppresses the host’s mutant phenotype.
Charles S. Hoffman, Robert Welton
doaj   +1 more source

Golden GATEway cloning--a combinatorial approach to generate fusion and recombination constructs. [PDF]

PLoS ONE, 2013
The design and generation of DNA constructs is among the necessary but generally tedious tasks for molecular biologists and, typically, the cloning strategy is restricted by available restriction sites.
Stephan Kirchmaier, Katharina Lust, Joachim Wittbrodt   +2 more
doaj   +1 more source

Rapid gene cloning in cereals [PDF]

Theoretical and Applied Genetics, 2018
The large and complex genomes of many cereals hindered cloning efforts in the past. Advances in genomics now allow the rapid cloning of genes from humanity's most valuable crops. The past two decades were characterized by a genomics revolution that entailed profound changes to crop research, plant breeding, and agriculture.
Bettgenhaeuser, Jan, Krattinger, Simon G.   +1 more
openaire   +3 more sources

Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3 [PDF]

, 2012
Large-scale production of plasmid DNA to prepare therapeutic gene vectors or DNA-based vaccines requires a suitable bacterial host, which can stably maintain the plasmid DNA during industrial cultivation.
Al-Allaf, FA, Mehmet, H, Tchetchelnitski, V, Tolmachov, OE, Zambetti, LP   +4 more
core   +1 more source

Nicotinamide N‐methyltransferase promotes drug resistance in lung cancer, as revealed by nascent proteomic profiling

Molecular Oncology, EarlyView.
AZD9291 has shown promise in targeted cancer therapy but is limited by resistance. In this study, we employed metabolic labeling and LC–MS/MS to profile time‐resolved nascent protein perturbations, allowing dynamic tracking of drug‐responsive proteins. We demonstrated that increased NNMT expression is associated with drug resistance, highlighting NNMT ...
Zhanwu Hou, Zhen Wang, Fei Yang, Xiao Han, Lei Li, Huadong Liu   +5 more
wiley   +1 more source

Functional analysis of the Arabidopsis TETRASPANIN gene family in plant growth and development [PDF]

, 2015
TETRASPANIN (TET) genes encode conserved integral membrane proteins that are known in animals to function in cellular communication during gamete fusion, immunity reaction and pathogen recognition.
Himanen, Kristiina, Muto, Antonella, Neyt, Pia, Van de Velde, Jan, Van Lijsebettens, Maria, Vandepoele, Klaas, Wang, Feng   +6 more
core   +1 more source