Results 91 to 100 of about 1,118,644 (205)

Increasing Gene Editing Efficiency via CRISPR/Cas9- or Cas12a-Mediated Knock-In in Primary Human T Cells

Biomedicines
T lymphocytes represent a promising target for genome editing. They are primarily modified to recognize and kill tumor cells or to withstand HIV infection. In most studies, T cell genome editing is performed using the CRISPR/Cas technology. Although this
Natalia Kruglova, Mikhail Shepelev
doaj   +1 more source

Targeted mutagenesis of FaTM6 in the Octoploid Strawberry (Fragaria x ananassa) using the CRISPR/Cas9 System [PDF]

, 2018
The B-class of MADS-box transcription factors has been studied in many plant species, but remain functionally uncharacterized in the Rosaceae family.
Duarte-Conde, José Antonio, Martín-Pizarro, Carmen, Posé, David   +2 more
core  

Generation and transcriptomic characterization of MIR137 knockout miniature pig model for neurodevelopmental disorders

Cell & Bioscience
Background Neurodevelopmental disorders (NDD), such as autism spectrum disorders (ASD) and intellectual disorders (ID), are highly debilitating childhood psychiatric conditions.
Shengyun Xu, Jiaoxiang Wang, Kexin Mao, Deling Jiao, Zhu Li, Heng Zhao, Yifei Sun, Jin Feng, Yuanhao Lai, Ruiqi Peng, Yu Fu, Ruoyi Gan, Shuhan Chen, Hong-Ye Zhao, Hong-Jiang Wei, Ying Cheng   +15 more
doaj   +1 more source

Gene editing & stem cells [PDF]

Journal of Cystic Fibrosis, 2018
Patrick T, Harrison, Nils, Hoppe, Ulrich, Martin   +2 more
openaire   +2 more sources

Refined DNA repair manipulation enables a universal knock-in strategy in mouse embryos

Nature Communications
The design and screening of sgRNA in CRISPR-dependent gene knock-in is always laborious. Therefore, a universal and highly efficient knock-in strategy suitable for different sgRNA target sites is necessary.
Hongyu Chen, Qingtong Tan, Li Li, Lanxin Li, Jiqiang Fu, Wencheng Zhu, Jie Li, Yining Wang, Shiyan Li, Huimin Li, Yidi Sun, Qiang Sun, Zongyang Lu, Zhen Liu   +13 more
doaj   +1 more source

N-terminus of Drosophila melanogaster MSL1 is critical for dosage compensation

eLife
The male-specific lethal complex (MSL), which consists of five proteins and two non-coding roX RNAs, is involved in the transcriptional enhancement of X-linked genes to compensate for the sex chromosome monosomy in Drosophila XY males compared with XX ...
Valentin Babosha, Natalia Klimenko, Anastasia Revel-Muroz, Evgeniya Tikhonova, Pavel Georgiev, Oksana Maksimenko   +5 more
doaj   +1 more source

Transfer of mitochondrial DNA into the nuclear genome during induced DNA breaks

Nature Communications
Mitochondria serve as the cellular powerhouse, and their distinct DNA makes them a prospective target for gene editing to treat genetic disorders. However, the impact of genome editing on mitochondrial DNA (mtDNA) stability remains a mystery.
Jinchun Wu, Yang Liu, Liqiong Ou, Tingting Gan, Zhengrong Zhangding, Shaopeng Yuan, Xinyi Liu, Mengzhu Liu, Jiasheng Li, Jianhang Yin, Changchang Xin, Ye Tian, Jiazhi Hu   +12 more
doaj   +1 more source

Genomic consequences of intensive inbreeding in miniature inbred pigs

BMC Genomics
Background Inbreeding, a central theme in evolutionary and conservation biology, is a crucial practice in breeding to stabilize and enhance the specific traits or to establish inbred lines.
Hong-Man Chen, Heng Zhao, Qun-Yao Zhu, Chen Yan, Ya-Qi Liu, Si Si, Muhammad Ameen Jamal, Kai-Xiang Xu, De-Ling Jiao, Min-Juan Lv, Wen Wang, Hong-Ye Zhao, Lei Chen, Ming-Shan Wang, Hong-Jiang Wei   +14 more
doaj   +1 more source

Letter to the editor. Gene editing and disabled people : a response to Iñigo de Miguel Beriain [PDF]

, 2020
Boardman, Felicity K.
core   +1 more source

<i>ANGPTL3</i> Gene Editing: Promise and Prudence. [PDF]

Eur Cardiol
Lorenzatti AJ.
europepmc   +1 more source