Results 71 to 80 of about 3,565,977 (300)

Decoding the dynamic extracellular matrix in cancer—3D models and bioscaffolds rewire the rules of tumor progression

FEBS Letters, EarlyView.
Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani, Konstantinos Spanopoulos, Zoi Piperigkou, Nikos K. Karamanos   +3 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Prime editing of Nectin-1 functional domains confers knockout-level resistance to pseudorabies virus

BMC Veterinary Research
Background The continuous evolution and cross-species transmission risk of pseudorabies virus (PRV) poses a substantial threat to the swine industry and public health.
Tangyuan Xie, Hanxi Ouyang, Yan Wang, Shuhui Zhang, Shuaikang Yang, Jing Kong, Tian Huang, Gengsheng Cao   +7 more
doaj   +1 more source

Efficient Delivery and Nuclear Uptake Is Not Sufficient to Detect Gene Editing in CD34+ Cells Directed by a Ribonucleoprotein Complex

Molecular Therapy: Nucleic Acids, 2018
CD34+ cells are prime targets for therapeutic strategies for gene editing, because modified progenitor cells have the capacity to differentiate through an erythropoietic lineage.
Shirin R. Modarai, Dula Man, Pawel Bialk, Natalia Rivera-Torres, Kevin Bloh, Eric B. Kmiec   +5 more
doaj   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Gene Editing and Genotoxicity: Targeting the Off-Targets [PDF]

, 2020
Gene editing technologies show great promise for application to human disease as a result of rapid developments in targeting tools notably based on ZFN, TALEN, and CRISPR-Cas systems. Precise modification of a DNA sequence is now possible in mature human
Blattner G., Turchiano G., Cavazza A., Thrasher A. J.   +3 more
core   +1 more source

Genotypic and Phenotypic Diversity as a Function of CRISPR-Directed Gene Knock-Out of NRF2 in Pancreatic Adenocarcinoma Cells, a Feasibility Study

Biomolecules
Pancreatic ductal adenocarcinoma (PDAC) presents unique treatment challenges, often due to the development of anti-cancer drug resistance. Previously, we demonstrated that CRISPR-directed gene ablation disabled the master regulator gene NRF2, a ...
London P. McGill, Kelly H. Banas, Gregory Tiesi, Eric B. Kmiec   +3 more
doaj   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Cisgenesis and genome editing: powerful tools for fruit breeding

, 2017
Over the past few years, the new plant breeding technologies emerged as outstanding tools in the scenario of the green biotechnologies all over the world.
Dalla Costa, Lorenza, Malnoy, Mickael Arnaud, Piazza, Stefano, Velasco, Riccardo   +3 more
core   +1 more source

Construction of TERT Monoallelic Knockout and TERT Overexpression of Porcine Cell Lines and Study of the Cellular Biological Characteristics

Animals
Telomerase reverse transcriptase subunit (TERT) is a key factor involved in telomere maintenance and genome stability, and the decline in its expression is closely related to cellular senescence.
Yanhong Yang, Xiaojing Chen, Jing Wang, Jingjing Xiong, Xiaoyin Zhang, Jiaoxiang Wang, Weiwei Xu, Yubo Qing, Honghui Li, Hong-Ye Zhao   +9 more
doaj   +1 more source