Results 51 to 60 of about 8,657,992 (314)

Functional characterization of tumor-specific CRISPR-directed gene editing as a combinatorial therapy for the treatment of solid tumors

Molecular Therapy: Oncology
As we pursue clinical applications for CRISPR-directed gene editing in overcoming resistance to anticancer drugs, we have focused on genetic disruption of the transcription factor, NRF2, a master regulator of cellular stress and detoxification. The level
Kelly H. Banas, Pawel A. Bialk, Natalia Rivera-Torres, Katelynn Owens, Tori N. Reiner, Kristen M. Pisarcik, Nicole Haas, Emily Gielda, Komal Khan, Krishna Priya Narra, Eric B. Kmiec   +10 more
doaj   +1 more source

Synergistic power of genomic selection, assisted reproductive technologies, and gene editing to drive genetic improvement of cattle

CABI Agriculture and Bioscience, 2022
Genetic improvement of cattle around the globe has been, and will continue to be, an important driver of animal agriculture sustainability. There are several reproductive and molecular biotechnologies that are used in genetic improvement of cattle, and ...
M. Mueller, Alison L. Van Eenennaam
semanticscholar   +1 more source

High-efficiency nonviral CRISPR/Cas9-mediated gene editing of human T cells using plasmid donor DNA

Journal of Experimental Medicine, 2022
Effective and precise gene editing of T lymphocytes is critical for advancing our understanding of T cell biology and the development of next-generation cellular therapies.
Soyoung A Oh, K. Senger, Shravan Madireddi, Ilseyar Akhmetzyanova, Isabel E Ishizuka, S. Tarighat, J. H. Lo, David Graeme Shaw, B. Haley, S. Rutz   +9 more
semanticscholar   +1 more source

Application of the CRISPR/Cas9-based gene editing technique in basic research, diagnosis, and therapy of cancer

Molecular Cancer, 2021
The 2020 Nobel Prize in Chemistry was awarded to Emmanuelle Charpentier and Jennifer Doudna for the development of the Clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease9 (CRISPR/Cas9) gene editing technology that ...
Huimin Zhang, Chunhong Qin, C. An, Xiwang Zheng, S. Wen, Wenjie Chen, Xianfang Liu, Zhenghua Lv, Pingchang Yang, Wei Xu, Wei Gao, Yongyan Wu   +11 more
semanticscholar   +1 more source

Target choice and exon skipping regulate CRISPR-directed gene editing of NRF2 in head/neck and esophageal cancer cells

Molecular Therapy: Oncology
Head and neck cancer (HNC) is the seventh most diagnosed cancer, with a predicted 30% increase annually by 2030. Conventional treatment is often combinatorial involving chemotherapy and radiation therapy, immunotherapy, and surgery, but over time ...
Natalia Rivera-Torres, Lauren E. Skelly, John A. Rogowskyj, Guadalupe Aguilar, Kelly Banas, Pawel Bialk, Eric B. Kmiec   +6 more
doaj   +1 more source

dCas9-based gene editing for cleavage-free genomic knock-in of long sequences

Nature Cell Biology, 2022
Gene editing is a powerful tool for genome and cell engineering. Exemplified by CRISPR–Cas, gene editing could cause DNA damage and trigger DNA repair processes that are often error-prone.
Chengkun Wang, Yuanhao Qu, Jason K. W. Cheng, Nicholas W. Hughes, Qianhe Zhang, Mengdi Wang, Le Cong   +6 more
semanticscholar   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Gene editing [PDF]

Nature Biotechnology, 2020
Clévio Nóbrega, Liliana Mendonça, Carlos A. Matos   +2 more
openaire   +3 more sources

Establishing an immersive educational experience in human gene editing

Discover Education
The transformative power of gene editing demands a corresponding transformation in its instruction. While commercial kits and curriculum-aligned tools have become available, they often offer a fragmented understanding, omitting both the mechanistic ...
Kristen M. Pisarcik, Amanda M. Hewes, Sarah A. LaTorre, Makenzie Sisson, John McDowell, Zaina Punter, Naiesha Brown, Jayme Del Mario, William Schmitt, Kelly H. Banas, Natalia Rivera-Torres, Eric B. Kmiec   +11 more
doaj   +1 more source

Construction and Functional Validation of GTKO/hCD55 Gene-Edited Xenotransplant Donor Pigs

Shiyan dongwu yu bijiao yixue
Objective To develop GTKO (α-1,3-galactosyltransferase gene-knockout, GTKO)/hCD55 (human CD55) gene-edited xenotransplant donor pigs and verify their function. Methods In this study, CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9
WANG Jiaoxiang, ZHANG Lu, CHEN Shuhan, JIAO Deling, ZHAO Heng, WEI Taiyun, GUO Jianxiong, XU Kaixiang, WEI Hongjiang   +8 more
doaj   +1 more source