Results 31 to 40 of about 1,135,829 (304)

Gene editing and gene regulation with CRISPR [PDF]

Experimental Physiology, 2018
Genome editing enables precise changes to be made in the genome of living cells. The technique was originally developed in the 1980’s but largely limited to use in mice. The discovery that a targeted double stranded break (DSB) at a unique site in the genome, close to the site to be changed, could substantially increase the efficiency of editing ...
Patrick Harrison, Stephen Hart
openaire   +3 more sources

Methods for Scarless, Selection-Free Generation of Human Cells and Allele-Specific Functional Analysis of Disease-Associated SNPs and Variants of Uncertain Significance. [PDF]

, 2017
With the continued emergence of risk loci from Genome-Wide Association studies and variants of uncertain significance identified from patient sequencing, better methods are required to translate these human genetic findings into improvements in public ...
Carvajal-Carmona, Luis G, Coggins, Nicole B, O'Geen, Henriette, Segal, David J, Stultz, Jacob   +4 more
core   +4 more sources

Gene editing restores dystrophin expression in a canine model of Duchenne muscular dystrophy [PDF]

, 2018
Mutations in the gene encoding dystrophin, a protein that maintains muscle integrity and function, cause Duchenne muscular dystrophy (DMD). The deltaE50-MD dog model of DMD harbors a mutation corresponding to a mutational “hotspot” in the human DMD gene.
Amoasii, L, Bassel-Duby, R, Caballero, D, Harron, R, Hildyard, J C W, Li, H, Massey, C A, Mireault, A, Olson, E N, Piercy, R J, Sanchez-Ortiz, E, Shelton, J M, Stathopoulou, T-R   +12 more
core   +2 more sources

Functional characterization of tumor-specific CRISPR-directed gene editing as a combinatorial therapy for the treatment of solid tumors

Molecular Therapy: Oncology
As we pursue clinical applications for CRISPR-directed gene editing in overcoming resistance to anticancer drugs, we have focused on genetic disruption of the transcription factor, NRF2, a master regulator of cellular stress and detoxification. The level
Kelly H. Banas, Pawel A. Bialk, Natalia Rivera-Torres, Katelynn Owens, Tori N. Reiner, Kristen M. Pisarcik, Nicole Haas, Emily Gielda, Komal Khan, Krishna Priya Narra, Eric B. Kmiec   +10 more
doaj   +1 more source

Gene editing

Postępy Biochemii, 2018
Development of the gene engineering techniques has raised worries that they will be used for construction of organism endangering humansand environment. In 1975 at the Asilomar conference, geneticists from many countries decided that genetic engineering brings more benefitsthan threats. In last years a new CRISPR-Cas technique emerged .
openaire   +3 more sources

Anti-CRISPR-mediated control of gene editing and synthetic circuits in eukaryotic cells. [PDF]

, 2019
Repurposed CRISPR-Cas molecules provide a useful tool set for broad applications of genomic editing and regulation of gene expression in prokaryotes and eukaryotes. Recent discovery of phage-derived proteins, anti-CRISPRs, which serve to abrogate natural
Abbott, Timothy R, Bondy-Denomy, Joseph, Carter, Matthew A, Chavez, Michael, Dominguez, Antonia A, Gao, Yuchen, Kipniss, Nathan H, La Russa, Marie, Lau, Matthew B, Nakamura, Muneaki, Qi, Lei S, Smolke, Christina D, Srinivasan, Prashanth, Xu, Xiaoshu, Zhao, Dehua   +14 more
core   +3 more sources

CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway. [PDF]

, 2020
With its high efficiency for site-specific genome editing and easy manipulation, the clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR associated protein 9 (CAS9) system has become the most widely used gene editing technology in ...
Chen, Qu, Fu, Xuemei, Kim, Jinchul, Liu, Jingfeng, Tang, Qingshuang, Xu, Shuxiang, Xu, Yang   +6 more
core  

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair. [PDF]

, 2020
Repair of double strand DNA breaks (DSBs) can result in gene disruption or gene modification via homology directed repair (HDR) from donor DNA. Altering cellular responses to DSBs may rebalance editing outcomes towards HDR and away from other repair ...
A Ferreira, A Montagnoli, A Orthwein, AC Komor, B Roberts, B Verdoodt, C Anders, C Yu, CD Richardson, CD Richardson, D Szklarczyk, DW Huang, E Lingeman, ES Koltun, FK Zimmermann, H Ghezraoui, J Song, JA Doudna, JE Haber, JP Duxin, K Jayathilaka, K Nakanishi, K Okita, KJ Livak, L Lei, L Pessoa-Brandão, LA Gilbert, LN Brandão, M Cavazzana, M Lei, M Yamada, MA DeWitt, MD Canny, MD Rainey, MH Porteus, N Hustedt, N Zielke, NK Sasi, NR Pannunzio, R Ceccaldi, RC Alver, S Hughes, S Lin, SB Thyme, T Maruyama, T Takenaka, T Tsuji, TA Day, TL Roth, TS Nambiar, U Eichenlaub-Ritter, VT Chu, W Li, X Liang, Z Romero   +54 more
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Target choice and exon skipping regulate CRISPR-directed gene editing of NRF2 in head/neck and esophageal cancer cells

Molecular Therapy: Oncology
Head and neck cancer (HNC) is the seventh most diagnosed cancer, with a predicted 30% increase annually by 2030. Conventional treatment is often combinatorial involving chemotherapy and radiation therapy, immunotherapy, and surgery, but over time ...
Natalia Rivera-Torres, Lauren E. Skelly, John A. Rogowskyj, Guadalupe Aguilar, Kelly Banas, Pawel Bialk, Eric B. Kmiec   +6 more
doaj   +1 more source

Live pigs produced from genome edited zygotes [PDF]

, 2013
Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome.
AJ Clark, AM Guerts, CJ Palgrave, D Yang, DF Carlson, FD Urnov, ID Carbery, J Hauschild, JC Miller, JJ Whyte, SC Fahrenkrug, T Flisokowska, VM Bedell, WS Tan   +13 more
core   +1 more source