Results 31 to 40 of about 916,088 (264)

Gene editing

Postępy Biochemii, 2018
Development of the gene engineering techniques has raised worries that they will be used for construction of organism endangering humansand environment. In 1975 at the Asilomar conference, geneticists from many countries decided that genetic engineering brings more benefitsthan threats. In last years a new CRISPR-Cas technique emerged .
openaire   +3 more sources

CRISPR/dCas9-Dnmt3a-mediated targeted DNA methylation of APP rescues brain pathology in a mouse model of Alzheimer’s disease

Translational Neurodegeneration, 2022
Background Aberrant DNA methylation patterns have been observed in neurodegenerative diseases, including Alzheimer's disease (AD), and dynamic changes in DNA methylation are closely associated with the onset and progression of these diseases ...
Hanseul Park, Jaein Shin, Yunkyung Kim, Takashi Saito, Takaomi C. Saido, Jongpil Kim   +5 more
doaj   +1 more source

Engineering T-Cell Resistance to HIV-1 Infection via Knock-In of Peptides from the Heptad Repeat 2 Domain of gp41

mBio, 2022
Previous studies suggest that short peptides from the heptad repeat 2 (HR2) domain of gp41 expressed on the cell surface are more potent inhibitors of HIV-1 entry than soluble analogs.
Alexandra Maslennikova, Natalia Kruglova, Svetlana Kalinichenko, Dmitriy Komkov, Mikhail Shepelev, Dmitriy Golubev, Andrei Siniavin, Andrei Vzorov, Alexander Filatov, Dmitriy Mazurov   +9 more
doaj   +1 more source

Strategies for mitochondrial gene editing

Computational and Structural Biotechnology Journal, 2021
Mitochondria, as the energy factory of cells, participate in metabolism processes and play a critical role in the maintenance of human life activities. Mitochondria belong to semi-automatic organelles, which have their own genome different from nuclear genome. Mitochondrial DNA (mtDNA) mutations can cause a series of diseases and threaten human health.
Xingbo Yang, Jiacheng Jiang, Zongyu Li, Jiayi Liang, Yaozu Xiang   +4 more
openaire   +3 more sources

Target choice and exon skipping regulate CRISPR-directed gene editing of NRF2 in head/neck and esophageal cancer cells

Molecular Therapy: Oncology
Head and neck cancer (HNC) is the seventh most diagnosed cancer, with a predicted 30% increase annually by 2030. Conventional treatment is often combinatorial involving chemotherapy and radiation therapy, immunotherapy, and surgery, but over time ...
Natalia Rivera-Torres, Lauren E. Skelly, John A. Rogowskyj, Guadalupe Aguilar, Kelly Banas, Pawel Bialk, Eric B. Kmiec   +6 more
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Gene editing [PDF]

Nature Biotechnology, 2020
Clévio Nóbrega, Liliana Mendonça, Carlos A. Matos   +2 more
openaire   +3 more sources

Establishing an immersive educational experience in human gene editing

Discover Education
The transformative power of gene editing demands a corresponding transformation in its instruction. While commercial kits and curriculum-aligned tools have become available, they often offer a fragmented understanding, omitting both the mechanistic ...
Kristen M. Pisarcik, Amanda M. Hewes, Sarah A. LaTorre, Makenzie Sisson, John McDowell, Zaina Punter, Naiesha Brown, Jayme Del Mario, William Schmitt, Kelly H. Banas, Natalia Rivera-Torres, Eric B. Kmiec   +11 more
doaj   +1 more source

Construction and Functional Validation of GTKO/hCD55 Gene-Edited Xenotransplant Donor Pigs

Shiyan dongwu yu bijiao yixue
Objective To develop GTKO (α-1,3-galactosyltransferase gene-knockout, GTKO)/hCD55 (human CD55) gene-edited xenotransplant donor pigs and verify their function. Methods In this study, CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9
WANG Jiaoxiang, ZHANG Lu, CHEN Shuhan, JIAO Deling, ZHAO Heng, WEI Taiyun, GUO Jianxiong, XU Kaixiang, WEI Hongjiang   +8 more
doaj   +1 more source

Gene editing CRISPR/Cas9 system for producing cows with hypoallergenic milk on the background of a beta-lactoglobulin gene knockout [PDF]

E3S Web of Conferences, 2020
Beta-lactoglobulin is the main allergen of cow’s milk. Modern approaches to reducing the allergenicity of milk require significant costs for its fermentation. An alternative approach could be the creation of productive breeds with a knockout for the gene
Silaeva Yulia Yu., Kubekina Marina V., Bruter Alexandra V., Isaeva Albina G., Koshchaev Andrey G.   +4 more
doaj   +1 more source