Results 41 to 50 of about 8,657,992 (314)
DIPA-CRISPR is a simple and accessible method for insect gene editing
Cell Reports Methods, 2022 Summary Current approaches for insect gene editing require microinjection of materials into early embryos. This severely limits the application of gene editing to a great number of insect species, especially to those whose reproduction systems preclude ...
Y. Shirai +3 more
semanticscholar +1 more source
Endogenous leptin promotes autophagy in EBSS-induced PFCs
Animal Cells and Systems, 2019 Leptin is an important adipokine and plays a vital role in animals. However, the role of leptin in the autophagic response of pig fibroblast cells (PFCs) has not been fully elucidated.
Deling Jiao +10 more
doaj +1 more source
mBio, 2022 Previous studies suggest that short peptides from the heptad repeat 2 (HR2) domain of gp41 expressed on the cell surface are more potent inhibitors of HIV-1 entry than soluble analogs.
Alexandra Maslennikova +9 more
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Engineered Cas9 extracellular vesicles as a novel gene editing tool
Journal of Extracellular Vesicles, 2022 Extracellular vesicles (EVs) have shown promise as biological delivery vehicles, but therapeutic applications require efficient cargo loading. Here, we developed new methods for CRISPR/Cas9 loading into EVs through reversible heterodimerization of Cas9 ...
X. Osteikoetxea +14 more
semanticscholar +1 more source
, 2023 Gene editing allows scientists to modify an organism's DNA with unparalleled precision, utilizing techniques like CRISPR-Cas9. It aids researchers to target specific genes, enhance desirable traits, correct genetic disorders, and develop innovative therapies.
openaire +3 more sources
CRISPR Gene Editing in the Kidney [PDF]
American Journal of Kidney Diseases, 2018 CRISPR is a nuclease guidance system that enables rapid and efficient gene editing of specific DNA sequences within genomes. We review applications of CRISPR for the study and treatment of kidney disease. CRISPR enables functional experiments in cell lines and model organisms to validate candidate genes arising from genetic studies.
Nelly M, Cruz, Benjamin S, Freedman
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Human genetic diversity alters off-target outcomes of therapeutic gene editing
Nature Genetics, 2022 CRISPR gene editing holds great promise to modify DNA sequences in somatic cells to treat disease. However, standard computational and biochemical methods to predict off-target potential focus on reference genomes.
Samuele Cancellieri +15 more
semanticscholar +1 more source
Gene editing CRISPR/Cas9 system for producing cows with hypoallergenic milk on the background of a beta-lactoglobulin gene knockout [PDF]
E3S Web of Conferences, 2020 Beta-lactoglobulin is the main allergen of cow’s milk. Modern approaches to reducing the allergenicity of milk require significant costs for its fermentation. An alternative approach could be the creation of productive breeds with a knockout for the gene
Silaeva Yulia Yu. +4 more
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Postępy Biochemii, 2018 Development of the gene engineering techniques has raised worries that they will be used for construction of organism endangering humansand environment. In 1975 at the Asilomar conference, geneticists from many countries decided that genetic engineering brings more benefitsthan threats. In last years a new CRISPR-Cas technique emerged .
openaire +3 more sources
CRISPR/Cas9 gene editing: a new approach for overcoming drug resistance in cancer
Cellular & Molecular Biology Letters, 2022 The CRISPR/Cas9 system is an RNA-based adaptive immune system in bacteria and archaea. Various studies have shown that it is possible to target a wide range of human genes and treat some human diseases, including cancers, by the CRISPR/Cas9 system.
Mostafa Vaghari-Tabari +7 more
semanticscholar +1 more source