Gene Editing of Primary Rhesus Macaque B Cells [PDF]
, 2023 B cells and their progeny are the sources of highly expressed antibodies. Their high protein expression capabilities together with their abundance, easy accessibility via peripheral blood, and amenability to simple adoptive transfers have made them an ...
Nussenzweig, Michel C. +8 more
core +1 more source
CRISPR-Click Enables Multi-Gene Editing with Modular Synthetic sgRNAs [PDF]
, 2021 Gene editing systems such as CRISPR/Cas9 readily enable individual gene phenotypes to be studied through loss-of-function. However, in certain instances, gene compensation can obfuscate the results of these studies, necessitating the editing of multiple ...
Matthew , Macauley +9 more
core +1 more source
Strategies for Efficient Gene Editing in Protoplasts of Solanum tuberosum Theme: Determining gRNA Efficiency Design by Utilizing Protoplast [PDF]
, 2022 Potato (Solanum tuberosum) is a highly diverse tetraploid crop. Elite cultivars are extremely heterozygous with a high prevalence of small length polymorphisms (indels) and single nucleotide polymorphisms (SNPs) within and between cultivars, which must ...
Kieu Phuong, Nam, +3 more
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Using Gene Editing Strategies for Wheat Improvement [PDF]
, 2023 Despite wheat’s global importance, it has trailed behind the other major cereals regarding genomic tools and resources as well as gene transformation.
Camerlengo, Francesco +7 more
core +1 more source
Tumor evolution selectively inactivates the core microRNA machinery for immune evasion
Nature Communications, 2021 Dysregulation of the microRNA machinery has crucial roles in cancer development. Here the authors show that inactivation of proteins involved in microRNA-mediated gene silencing, such as ANKRD52 or AGO2, confers resistance to T cell-mediated immune ...
Tian-Yu Song +15 more
doaj +1 more source
Alternative splicing and extensive RNA editing of human TPH2 transcripts. [PDF]
, 2010 Brain serotonin (5-HT) neurotransmission plays a key role in the regulation of mood and has been implicated in a variety of neuropsychiatric conditions. Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the biosynthesis of 5-HT.
Bondy, Brigitta +59 more
core +1 more source
Innovative Precision Gene‐Editing Tools in Personalized Cancer Medicine [PDF]
, 2020 The development of clustered regularly interspaced short palindromic repeats (CRISPR) has spurred a successive wave of genome‐engineering following zinc finger nucleases and transcription activator‐like effector nucleases, and made gene‐editing a ...
Peiyu Wang +11 more
core +1 more source
Gene Editing by Extracellular Vesicles [PDF]
International Journal of Molecular Sciences, 2020 CRISPR/Cas technologies have advanced dramatically in recent years. Many different systems with new properties have been characterized and a plethora of hybrid CRISPR/Cas systems able to modify the epigenome, regulate transcription, and correct mutations in DNA and RNA have been devised.
Dmitry Kostyushev +6 more
openaire +2 more sources
Gene editing and gene drive on livestock: a bioethical analysis [PDF]
, 2022 reservedCRISPR-Cas9 è un’innovativa tecnica di gene editing, nota anche come “taglia e cuci” del DNA. Questa tecnica è stata sviluppata nel 2012 dalle due ricercatrici Emmanuelle Charpentier e Jennifer Anne Doudna e, grazie alla sua rilevanza ...
TOSETTO, GIULIA
core
An essential role of KREPB4 in RNA editing and structural integrity of the editosome in Trypanosoma brucei [PDF]
, 2007 RNA editing in the sleeping sickness parasite Trypanosoma brucei remodels mitochondrial transcripts by the addition and deletion of uridylates as specified by guide RNAs.
Schnaufer, Achim; id_orcid +4 more
core +1 more source