Results 11 to 20 of about 6,790,032 (188)

Gene Electrotransfer into Mammalian Cells Using Commercial Cell Culture Inserts with Porous Substrate. [PDF]

Pharmaceutics, 2022
Gene electrotransfer is one of the main non-viral methods for intracellular delivery of plasmid DNA, wherein pulsed electric fields are used to transiently permeabilize the cell membrane, allowing enhanced transmembrane transport.
Vindiš T, Blažič A, Khayyat D, Potočnik T, Sachdev S, Rems L.   +5 more
europepmc   +2 more sources

Systemic Optimization of Gene Electrotransfer Protocol Using Hard-to-Transfect UT-7 Cell Line as a Model. [PDF]

Biomedicines, 2022
Non-adherent cells are difficult to transfect with chemical-mediated delivery methods. Electroporation is an attractive strategy to transfer the molecules of interest into suspension cells.
Vadeikienė R, Jakštys B, Ugenskienė R, Šatkauskas S, Juozaitytė E.   +4 more
europepmc   +2 more sources

Treatment of skin tumors with intratumoral interleukin 12 gene electrotransfer in the head and neck region: a first-in-human clinical trial protocol. [PDF]

Radiol Oncol, 2022
Background Immune therapies are currently under intensive investigation providing in many cases excellent responses in different tumors. Other possible approach for immunotherapy is a targeted intratumoral delivery of interleukin 12 (IL-12), a cytokine ...
Groselj A, Bosnjak M, Jesenko T, Cemazar M, Markelc B, Strojan P, Sersa G.   +6 more
europepmc   +2 more sources

Gene electrotransfer with flow-through microchannel and lower alternating voltage generated induced pluripotent cells from human lymphoblastoid cell lines. [PDF]

PLoS One
Induced pluripotent stem cells (iPSCs) are useful for studying genetic and rare diseases and can be generated by reprogramming immortalized lymphoblastoid cell lines (LCLs) stored in global repositories with detailed genotype and phenotype data ...
Ishii-Teshima M, Maeda K, Hanauchi K, Asechi E, Setyawan D, Niki T, Nakashima K, Kurita H, Numano R, Shibata T.   +9 more
europepmc   +2 more sources

Interplay between Electric Field Strength and Number of Short-Duration Pulses for Efficient Gene Electrotransfer. [PDF]

Pharmaceuticals (Basel)
Electroporation is a method that shows great promise as a non-viral approach for delivering genes by using high-voltage electric pulses to introduce DNA into cells to induce transient gene expression. This research aimed to evaluate the interplay between
Urbanskas E, Jakštys B, Venckus J, Malakauskaitė P, Šatkauskienė I, Morkvėnaitė-Vilkončienė I, Šatkauskas S.   +6 more
europepmc   +2 more sources

Feasibility Study for the Use of Gene Electrotransfer and Cell Electrofusion as a Single-Step Technique for the Generation of Activated Cancer Cell Vaccines. [PDF]

J Membr Biol
Cell-based therapies hold great potential for cancer immunotherapy. This approach is based on manipulation of dendritic cells to activate immune system against specific cancer antigens.
Ušaj M, Pavlin M, Kandušer M.
europepmc   +2 more sources

Gene Electrotransfer via Conductivity-Clamped Electric Field Focusing Pivots Sensori-Motor DNA Therapeutics: "A Spoonful of Sugar Helps the Medicine Go Down". [PDF]

Adv Sci (Weinh)
Viral vectors and lipofection‐based gene therapies have dispersion‐dependent transduction/transfection profiles that thwart precise targeting. The study describes the development of focused close‐field gene electrotransfer (GET) technology, refining ...
Pinyon JL, von Jonquieres G, Crawford EN, Abed AA, Power JM, Klugmann M, Browne CJ, Housley DM, Wise AK, Fallon JB, Shepherd RK, Lin JY, McMahon C, McAlpine D, Birman CS, Lai W, Enke YL, Carter PM, Patrick JF, Gay RD, Marie C, Scherman D, Lovell NH, Housley GD.   +23 more
europepmc   +2 more sources

The impact of impaired DNA mobility on gene electrotransfer efficiency: analysis in 3D model. [PDF]

Biomed Eng Online, 2021
Gene electrotransfer is an established method that enables transfer of DNA into cells with electric pulses. Several studies analyzed and optimized different parameters of gene electrotransfer, however, one of main obstacles toward efficient ...
Meglič SH, Pavlin M.
europepmc   +2 more sources

Moderate Heat-Assisted Gene Electrotransfer as a Potential Delivery Approach for Protein Replacement Therapy through the Skin. [PDF]

Pharmaceutics, 2021
Gene-based approaches for protein replacement therapies have the potential to reduce the number of administrations. Our previous work demonstrated that expression could be enhanced and/or the applied voltage reduced by preheating the tissue prior to ...
Edelblute C, Mangiamele C, Heller R.
europepmc   +2 more sources

Vector‐Free Deep Tissue Targeting of DNA/RNA Therapeutics via Single Capacitive Discharge Conductivity‐Clamped Gene Electrotransfer (Adv. Sci. 3/2025) [PDF]

Advanced science
DNA/RNA Therapeutics Single pulse electrotransfer of ‘naked’ DNA / mRNA via electric field focusing for deep tissue and vaccine therapeutics. In article 2406545, Jeremy L. Pinyon, Georg von Jonquieres, Gary D. Housley, and co‐workers describe vector‐free
Jeremy L. Pinyon, Georg von Jonquieres, Stephen L. Mow, A. A. Abed, Keng‐Yin Lai, Mathumathi Manoharan, Edward N. Crawford, Stanley H Xue, Sarah Smith‐Moore, Lisa J. Caproni, Sarah Milsom, Matthias Klugmann, Nigel H. Lovell, G. Housley   +13 more
europepmc   +2 more sources