Tumor Radiosensitization by Gene Electrotransfer-Mediated Double Targeting of Tumor Vasculature. [PDF]
Targeting the tumor vasculature through specific endothelial cell markers involved in different signaling pathways represents a promising tool for tumor radiosensitization. Two prominent targets are endoglin (CD105), a transforming growth factor β co-receptor, and the melanoma cell adhesion molecule (CD1046), present also on many tumors.
Savarin M +5 more
europepmc +3 more sources
Gene Electrotransfer: A Mechanistic Perspective. [PDF]
Gene electrotransfer is a powerful method of DNA delivery offering several medical applications, among the most promising of which are DNA vaccination and gene therapy for cancer treatment. Electroporation entails the application of electric fields to cells which then experience a local and transient change of membrane permeability.
Rosazza C +4 more
europepmc +4 more sources
Thermal Assisted In Vivo Gene Electrotransfer. [PDF]
Gene electrotransfer is an effective approach for delivering plasmid DNA to a variety of tissues. Delivery of molecules with electric pulses requires control of the electrical parameters to achieve effective delivery. Since discomfort or tissue damage may occur with high applied voltage, the reduction of the applied voltage while achieving the desired ...
Donate A +8 more
europepmc +5 more sources
Treatment of skin tumors with intratumoral interleukin 12 gene electrotransfer in the head and neck region: a first-in-human clinical trial protocol [PDF]
Immune therapies are currently under intensive investigation providing in many cases excellent responses in different tumors. Other possible approach for immunotherapy is a targeted intratumoral delivery of interleukin 12 (IL-12), a cytokine with anti ...
Groselj Ales +6 more
doaj +2 more sources
Numerical study of gene electrotransfer efficiency based on electroporation volume and electrophoretic movement of plasmid DNA [PDF]
Background The efficiency of gene electrotransfer, an electroporation-based method for delivery of pDNA into target tissues, depends on several processes.
Tadeja Forjanič, Damijan Miklavčič
doaj +2 more sources
Vector‐Free Deep Tissue Targeting of DNA/RNA Therapeutics via Single Capacitive Discharge Conductivity‐Clamped Gene Electrotransfer [PDF]
Viral vector and lipid nanoparticle based gene delivery have limitations around spatiotemporal control, transgene packaging size, and vector immune reactivity, compromising translation of nucleic acid (NA) therapeutics.
Jeremy L. Pinyon +13 more
doaj +2 more sources
Limited Efficacy of Nanoparticle-Assisted Electroporation for Membrane Permeabilization and Gene Electrotransfer [PDF]
Background/Objectives: Nanoparticles (NPs) were previously explored as enhancers in electroporation due to their potential to locally amplify electric fields near cell membranes, with gold nanoparticles (AuNPs) in particular showing promise in improving ...
Tamara Polajžer +7 more
doaj +2 more sources
Gene electrotransfer enhanced by nanosecond pulsed electric fields
The impact of nanosecond pulsed electric fields (nsPEFs) on gene electrotransfer has not been clearly demonstrated in previous studies. This study was conducted to evaluate the influence of nsPEFs on the delivery of plasmids encoding luciferase or green ...
Siqi Guo +5 more
doaj +4 more sources
Effects of electrochemotherapy with cisplatin and peritumoral IL-12 gene electrotransfer on canine mast cell tumors: a histopathologic and immunohistochemical study [PDF]
The study was aimed to characterize tumor response after combined treatment employing electrochemotherapy with IL-12 gene electrotransfer in dogs with spontaneous mast cell tumors (MCT).
Salvadori Claudia +9 more
doaj +2 more sources
Recellularized human dermis for testing gene electrotransfer ex vivo. [PDF]
Gene electrotransfer (GET) is a proven and valuable tool for in vivo gene delivery to a variety of tissues such as skin, cardiac muscle, skeletal muscle, and tumors, with controllable gene delivery and expression levels. Optimizing gene expression is a challenging hurdle in preclinical studies, particularly for skin indications, due to differences in ...
Bulysheva AA +5 more
europepmc +4 more sources

