Results 41 to 50 of about 2,697,950 (180)

ELECTROTRANSFER OF CYTOKINE GENES FOR CANCER TREATMENT

CBU International Conference Proceedings, 2018
Gene electrotransfer, which designates the combination of gene transfer and electroporation, is a physical method for transfecting genes into cells and tissues. Many reports for the utilization of this techniques in animals confirmed that gene electrotransfer is a safe and efficient method.
Chopra, Sonam, Šatkauskas, Saulius
openaire   +2 more sources

In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle Tissue

Biological Procedures Online, 2009
DNA electrotransfer to muscle tissue yields long-term, high levels of gene expression; showing great promise for future gene therapy. We want to characterize the novel far-red fluorescent protein Katushka as a marker for gene expression using time domain
Eriksen Jens, Gehl Julie, Hojman Pernille   +2 more
doaj   +3 more sources

Immune cell populations in the tumour environment following calcium electropora­tion for cutaneous metastasis: a histopathological study

Acta Oncologica
Background and Purpose: Calcium electroporation (CaEP) involves injecting calcium into tumour tissues and using electrical pulses to create membrane pores that induce cell death.
Mille Vissing, Sandra Sinius Pouplier, Lars Munch Larsen, Stine Krog Frandsen, Alexey Lodin, Anne-Vibeke Lænkholm, Julie Gehl   +6 more
doaj   +1 more source

Electroporator with automatic change of electric field direction improves gene electrotransfer in-vitro

BioMedical Engineering OnLine, 2007
Background Gene electrotransfer is a non-viral method used to transfer genes into living cells by means of high-voltage electric pulses. An exposure of a cell to an adequate amplitude and duration of electric pulses leads to a temporary increase of cell ...
Rols Marie-Pierre, Teissié Justin, Čorović Selma, Kandušer Maša, Faurie Cécile, Reberšek Matej, Miklavčič Damijan   +6 more
doaj   +1 more source

Gene electrotransfer of FGF2 enhances collagen scaffold biocompatibility

Bioelectrochemistry, 2022
Tendon injuries are a common athletic injury that have been increasing in prevalence. While there are current clinical treatments for tendon injuries, they have relatively long recovery times and often do not restore native function of the tendon. In the current study, gene electrotransfer (GET) parameters for delivery to the skin were optimized with ...
Carly Boye, Kyle Christensen, Kamal Asadipour, Scott DeClemente, Michael Francis, Anna Bulysheva   +5 more
openaire   +3 more sources

Electrotransfer of plasmid DNA radiosensitizes B16F10 tumors through activation of immune response

Radiology and Oncology, 2017
Tumor irradiation combined with adjuvant treatments, either vascular targeted or immunomodulatory, is under intense investigation. Gene electrotransfer of therapeutic genes is one of these approaches.
Savarin Monika, Kamensek Urska, Cemazar Maja, Heller Richard, Sersa Gregor   +4 more
doaj   +1 more source

Non-Clinical In Vitro Evaluation of Antibiotic Resistance Gene-Free Plasmids Encoding Human or Murine IL-12 Intended for First-in-Human Clinical Study

Pharmaceutics, 2021
Interleukin 12 (IL-12) is a key cytokine that mediates antitumor activity of immune cells. To fulfill its clinical potential, the development is focused on localized delivery systems, such as gene electrotransfer, which can provide localized delivery of ...
Spela Kos, Masa Bosnjak, Tanja Jesenko, Bostjan Markelc, Urska Kamensek, Katarina Znidar, Urska Matkovic, Andrej Rencelj, Gregor Sersa, Rosana Hudej, Aneja Tuljak, Matjaz Peterka, Maja Cemazar   +12 more
doaj   +1 more source

Gene expression profiles in skeletal muscle after gene electrotransfer-0

, 2011
Copyright information:Taken from "Gene expression profiles in skeletal muscle after gene electrotransfer"http://www.biomedcentral.com/1471-2199/8/56BMC Molecular Biology 2007;8():56-56.Published online 29 Jun 2007PMCID:PMC1925113.nsfer, and fixed and ...
Hanne Gissel (61815), Jens Eriksen (61816), Pernille Hojman (61813), John R Zibert (61814), Julie Gehl (61817)   +4 more
core   +1 more source

The Prodigious Potential of mRNA Electrotransfer as a Substitute to Conventional DNA-Based Transient Transfection

Cells, 2023
Transient transfection of foreign DNA is the most widely used laboratory technique to study gene function and product. However, the transfection efficiency depends on many parameters, including DNA quantity and quality, transfection methods and target ...
Théo Juncker, Bruno Chatton, Mariel Donzeau   +2 more
doaj   +1 more source

Detection of Mitochondrial Respiratory Chain Complexes by BN‐PAGE and Its Applications

Future Postharvest and Food, EarlyView.
Schematic workflow for the isolation and BN‐PAGE analysis of plant mitochondrial respiratory chain complexes. ABSTRACT Blue native polyacrylamide gel electrophoresis (BN‐PAGE) is a core technique for separating and characterizing plant mitochondrial respiratory chain complexes.
Jinghan Zhang, Jinyan Li, Hongliang Zhu
wiley   +1 more source