Results 41 to 50 of about 2,519,482 (174)

Overcoming the Specific Toxicity of Large Plasmids Electrotransfer in Primary Cells In Vitro

Molecular Therapy: Nucleic Acids, 2016
Gene electrotransfer is a safe and efficient nonviral technique for the transfer of nucleic acids of all sizes. Using a small reporter plasmid (3.5 kbp), electrotransfer of more than 90% of the cells, with ≃70% viability, can be routinely achieved even ...
Léa L Lesueur, Lluis M Mir, Franck M André   +2 more
doaj   +1 more source

Combined effect of heat and corona charge on molecular delivery to a T cell line in vitro.

PLoS ONE, 2023
With the rapid increase of gene and immunotherapies for treating cancer, there is a need to efficiently transfect cells. Previous studies suggest that electrotransfer can provide a non-viral method for gene delivery.
Molly A Skinner, Alex Otten, Andrew Hoff, Mark Jaroszeski   +3 more
doaj   +1 more source

ELECTROTRANSFER OF CYTOKINE GENES FOR CANCER TREATMENT

CBU International Conference Proceedings, 2018
Gene electrotransfer, which designates the combination of gene transfer and electroporation, is a physical method for transfecting genes into cells and tissues. Many reports for the utilization of this techniques in animals confirmed that gene electrotransfer is a safe and efficient method.
Chopra, Sonam, Šatkauskas, Saulius
openaire   +2 more sources

Neurotrophin gene augmentation by electrotransfer to improve cochlear implant hearing outcomes [PDF]

, 2019
This Review outlines the development of DNA-based therapeutics for treatment of hearing loss, and in particular, considers the potential to utilize the properties of recombinant neurotrophins to improve cochlear auditory (spiral ganglion) neuron survival
Marie, C, Duxbury, M, Shepherd, RK, Birman, CS, Fallon, JB, Lovell, NH, McAlpine, D, Carter, PM, Al Abed, A, Enke, YL, von Jonquieres, G, Wise, AK, Lai, W, Patrick, JF, McMahon, C, Klugmann, M, Scherman, D, Housley, GD, Pinyon, JL, Crawford, EN, Schilder, AGM   +20 more
core   +3 more sources

Expression of GFP and DsRed fluorescent proteins after gene electrotransfer of tumour cells in vitro

, 2023
Fluorescent reporter genes are widely used to study the transfection of various types of primary cells and cell lines. The aim of our research was to investigate the expression dynamics of GFP and DsRed reporter genes individually and combined after gene
Serša, Gregor, Bošnjak, Maša, Čemažar, Maja, Masa Bosnjak, Maja Cemazar, Komel, Tilen, Tilen Komel, Gregor Sersa   +7 more
core   +1 more source

In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle Tissue

Biological Procedures Online, 2009
DNA electrotransfer to muscle tissue yields long-term, high levels of gene expression; showing great promise for future gene therapy. We want to characterize the novel far-red fluorescent protein Katushka as a marker for gene expression using time domain
Eriksen Jens, Gehl Julie, Hojman Pernille   +2 more
doaj   +3 more sources

Immune cell populations in the tumour environment following calcium electropora­tion for cutaneous metastasis: a histopathological study

Acta Oncologica
Background and Purpose: Calcium electroporation (CaEP) involves injecting calcium into tumour tissues and using electrical pulses to create membrane pores that induce cell death.
Mille Vissing, Sandra Sinius Pouplier, Lars Munch Larsen, Stine Krog Frandsen, Alexey Lodin, Anne-Vibeke Lænkholm, Julie Gehl   +6 more
doaj   +1 more source

Gene electrotransfer of FGF2 enhances collagen scaffold biocompatibility

Bioelectrochemistry, 2022
Tendon injuries are a common athletic injury that have been increasing in prevalence. While there are current clinical treatments for tendon injuries, they have relatively long recovery times and often do not restore native function of the tendon. In the current study, gene electrotransfer (GET) parameters for delivery to the skin were optimized with ...
Carly Boye, Kyle Christensen, Kamal Asadipour, Scott DeClemente, Michael Francis, Anna Bulysheva   +5 more
openaire   +3 more sources

Electrotransfer of plasmid DNA radiosensitizes B16F10 tumors through activation of immune response

Radiology and Oncology, 2017
Tumor irradiation combined with adjuvant treatments, either vascular targeted or immunomodulatory, is under intense investigation. Gene electrotransfer of therapeutic genes is one of these approaches.
Savarin Monika, Kamensek Urska, Cemazar Maja, Heller Richard, Sersa Gregor   +4 more
doaj   +1 more source

Non-Clinical In Vitro Evaluation of Antibiotic Resistance Gene-Free Plasmids Encoding Human or Murine IL-12 Intended for First-in-Human Clinical Study

Pharmaceutics, 2021
Interleukin 12 (IL-12) is a key cytokine that mediates antitumor activity of immune cells. To fulfill its clinical potential, the development is focused on localized delivery systems, such as gene electrotransfer, which can provide localized delivery of ...
Spela Kos, Masa Bosnjak, Tanja Jesenko, Bostjan Markelc, Urska Kamensek, Katarina Znidar, Urska Matkovic, Andrej Rencelj, Gregor Sersa, Rosana Hudej, Aneja Tuljak, Matjaz Peterka, Maja Cemazar   +12 more
doaj   +1 more source