Overcoming the Specific Toxicity of Large Plasmids Electrotransfer in Primary Cells In Vitro
Molecular Therapy - Nucleic Acids, 2016 Gene electrotransfer is a safe and efficient nonviral technique for the transfer of nucleic acids of all sizes. Using a small reporter plasmid (3.5 kbp), electrotransfer of more than 90% of the cells, with ≃70% viability, can be routinely achieved even ...
Franck André
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Comprehensive Optimization of Western Blotting [PDF]
Gels, 2023 Western blotting is one of the most extensively used techniques in the biomedical field. However, it is criticized by many researchers due to its considerable time consumption, multiple steps, and low method results.
Dishiwen Liu +3 more
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Effective gene immunotherapy for melanoma utilizing an advanced in vivo electrotransfer system [PDF]
Molecular Therapy: OncologyInterleukin-12 (IL-12) is a potent immune stimulator that induces the proliferation and activation of natural killer (NK) and T cells and the secretion of interferon (IFN)-γ.
Loree C. Heller +8 more
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Pulsed Electric Fields Induce STING Palmitoylation and Polymerization Independently of Plasmid DNA Electrotransfer [PDF]
PharmaceuticsGene therapy approaches may target skeletal muscle due to its high protein-expressing nature and vascularization. Intramuscular plasmid DNA (pDNA) delivery via pulsed electric fields (PEFs) can be termed electroporation or electrotransfer.
Amanda Sales Conniff +3 more
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Interplay between Electric Field Strength and Number of Short-Duration Pulses for Efficient Gene Electrotransfer [PDF]
PharmaceuticalsElectroporation is a method that shows great promise as a non-viral approach for delivering genes by using high-voltage electric pulses to introduce DNA into cells to induce transient gene expression. This research aimed to evaluate the interplay between
Ernestas Urbanskas +6 more
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Electrotransfer parameters as a tool for controlled and targeted gene expression in skin
Molecular Therapy - Nucleic Acids, 2016 Skin is an attractive target for gene electrotransfer. It consists of different cell types that can be transfected, leading to various responses to gene electrotransfer. We demonstrate that these responses could be controlled by selecting the appropriate
Urška Kamensek +2 more
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Applied Sciences (Switzerland), 2022 Electroporation (EP) allows for the transport of molecules into the cytoplasm with significant effectiveness by forming transient pores in the cell membrane using electric pulses. This can be used for cellular transport (RE—reversible electroporation) or
Jolanta Saczko +2 more
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Endocytosis and Endosomal Trafficking of DNA After Gene Electrotransfer In Vitro
Molecular Therapy - Nucleic Acids, 2016 DNA electrotransfer is a successful technique for gene delivery into cells and represents an attractive alternative to virus-based methods for clinical applications including gene therapy and DNA vaccination.
Andreas Zumbusch +2 more
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Vector‐Free Deep Tissue Targeting of DNA/RNA Therapeutics via Single Capacitive Discharge Conductivity‐Clamped Gene Electrotransfer [PDF]
Advanced ScienceViral vector and lipid nanoparticle based gene delivery have limitations around spatiotemporal control, transgene packaging size, and vector immune reactivity, compromising translation of nucleic acid (NA) therapeutics.
Jeremy L. Pinyon +13 more
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The impact of impaired DNA mobility on gene electrotransfer efficiency: analysis in 3D model
BioMedical Engineering OnLine, 2021 Background Gene electrotransfer is an established method that enables transfer of DNA into cells with electric pulses. Several studies analyzed and optimized different parameters of gene electrotransfer, however, one of main obstacles toward efficient ...
Saša Haberl Meglič, Mojca Pavlin
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