Results 21 to 30 of about 5,598 (174)
Pharmaceutics, 2021 Gene-based approaches for protein replacement therapies have the potential to reduce the number of administrations. Our previous work demonstrated that expression could be enhanced and/or the applied voltage reduced by preheating the tissue prior to ...
Chelsea Edelblute +2 more
doaj +1 more source
Noninvasive Gene Electrotransfer in Skin [PDF]
Human Gene Therapy Methods, 2019 The skin is considered as well suited for gene therapy and vaccination. DNA vaccines elicit both broad humoral and cellular immune responses when injected in the skin. Physical and chemical methods are needed to boost the expression. Gene electrotransfer (GET) is one of the most effective approaches.
Lise, Pasquet +5 more
openaire +2 more sources
Gene Electrotransfer: A Mechanistic Perspective [PDF]
Current Gene Therapy, 2016 Gene electrotransfer is a powerful method of DNA delivery offering several medical applications, among the most promising of which are DNA vaccination and gene therapy for cancer treatment. Electroporation entails the application of electric fields to cells which then experience a local and transient change of membrane permeability.
Rosazza, Christelle +4 more
openaire +2 more sources
Radiosensitizing effect of intratumoral interleukin-12 gene electrotransfer in murine sarcoma
BMC Cancer, 2013 Background Interleukin-12 (IL-12) based radiosensitization is an effective way of tumor treatment. Local cytokine production, without systemic shedding, might provide clinical benefit in radiation treatment of sarcomas.
Sedlar Ales +5 more
doaj +1 more source
The Influence of Calcium Ions on the Electrotransfer Efficiency of Plasmid DNA and Cell Viability
Applied Sciences, 2023 Gene electrotransfer (GET) is recognized as a promising technique for the development of an efficient tool for gene therapy. Such a therapy would have applications in the treatment of a variety of genetic diseases, including cancer.
Rūta Palepšienė +4 more
doaj +1 more source
Molecular Therapy: Nucleic Acids, 2012 Electroporation (EP) of mammalian tissue is a technique that has been used successfully in the clinic for the delivery of genetic-based vaccines in the form of DNA plasmids.
Kate E Broderick +8 more
doaj +1 more source
Biomedicines, 2022 Non-adherent cells are difficult to transfect with chemical-mediated delivery methods. Electroporation is an attractive strategy to transfer the molecules of interest into suspension cells.
Roberta Vadeikienė +4 more
doaj +1 more source
In vivo DNA electrotransfer into muscle [PDF]
Development, Growth & Differentiation, 2008 Naked plasmid DNA injected into skeletal muscle is taken up by muscle cells and the genes in the plasmid are expressed. Among the non‐viral techniques for gene transfer in vivo, this method is especially simple, inexpensive, and safe. However, the relatively low expression levels attained by this method have limited its applications for uses other than
Satsuki, Miyazaki, Jun-Ichi, Miyazaki
openaire +2 more sources
Mediators of Inflammation, 2017 Cancer immunotherapy is currently one of the leading approaches in cancer treatment. Gene electrotransfer of plasmids encoding interleukin 12 (IL-12) into the cells leads to the production of IL-12, which drives immune cell polarization to an antitumoral
Ursa Lampreht Tratar +6 more
doaj +1 more source
Molecular Therapy: Methods & Clinical Development, 2014 DNA vaccination consists in administering an antigen-encoding plasmid in order to trigger a specific immune response. This specific vaccine strategy is of particular interest to fight against various infectious diseases and cancer.
Christophe Y Calvet +7 more
doaj +1 more source