Results 21 to 30 of about 5,729 (171)

Optimization of Mouse Growth Hormone Plasmid DNA Electrotransfer into Tibialis Cranialis Muscle of “Little” Mice

Molecules, 2020
Previous non-viral gene therapy was directed towards two animal models of dwarfism: Immunodeficient (lit/scid) and immunocompetent (lit/lit) dwarf mice.
Eliana Rosa Lima, Claudia Regina Cecchi, Eliza Higuti, Gustavo Protasio Pacheco de Jesus, Alissandra Moura Gomes, Enio Aparecido Zacarias, Paolo Bartolini, Cibele Nunes Peroni   +7 more
doaj   +1 more source

Dielectrophoretic Manipulation of Cell Transfection Efficiency during Electroporation Using a Center Needle Electrode

Applied Sciences, 2021
Long duration electric pulses are frequently used to facilitate DNA electrotransfer into cells and tissues, while electroporation pulses can be combined with electrophoresis to maximize the transfection efficiency.
Eivina Radzevičiūtė, Arūnas Murauskas, Paulius Ruzgys, Saulius Šatkauskas, Irutė Girkontaitė, Jurij Novickij, Vitalij Novickij   +6 more
doaj   +1 more source

Moderate Heat-Assisted Gene Electrotransfer as a Potential Delivery Approach for Protein Replacement Therapy through the Skin

Pharmaceutics, 2021
Gene-based approaches for protein replacement therapies have the potential to reduce the number of administrations. Our previous work demonstrated that expression could be enhanced and/or the applied voltage reduced by preheating the tissue prior to ...
Chelsea Edelblute, Cathryn Mangiamele, Richard Heller   +2 more
doaj   +1 more source

Gene Electrotransfer: A Mechanistic Perspective [PDF]

Current Gene Therapy, 2016
Gene electrotransfer is a powerful method of DNA delivery offering several medical applications, among the most promising of which are DNA vaccination and gene therapy for cancer treatment. Electroporation entails the application of electric fields to cells which then experience a local and transient change of membrane permeability.
Rosazza, Christelle, Meglic, Sasa Haberl, Zumbusch, Andreas, Rols, Marie-Pierre, Miklavcic, Damijan   +4 more
openaire   +2 more sources

Radiosensitizing effect of intratumoral interleukin-12 gene electrotransfer in murine sarcoma

BMC Cancer, 2013
Background Interleukin-12 (IL-12) based radiosensitization is an effective way of tumor treatment. Local cytokine production, without systemic shedding, might provide clinical benefit in radiation treatment of sarcomas.
Sedlar Ales, Kranjc Simona, Dolinsek Tanja, Cemazar Maja, Coer Andrej, Sersa Gregor   +5 more
doaj   +1 more source

The Influence of Calcium Ions on the Electrotransfer Efficiency of Plasmid DNA and Cell Viability

Applied Sciences, 2023
Gene electrotransfer (GET) is recognized as a promising technique for the development of an efficient tool for gene therapy. Such a therapy would have applications in the treatment of a variety of genetic diseases, including cancer.
Rūta Palepšienė, Martynas Maciulevičius, Paulius Ruzgys, Baltramiejus Jakštys, Saulius Šatkauskas   +4 more
doaj   +1 more source

Optimized In Vivo Transfer of Small Interfering RNA Targeting Dermal Tissue Using In Vivo Surface Electroporation

Molecular Therapy: Nucleic Acids, 2012
Electroporation (EP) of mammalian tissue is a technique that has been used successfully in the clinic for the delivery of genetic-based vaccines in the form of DNA plasmids.
Kate E Broderick, Amy Chan, Feng Lin, Xuefei Shen, Gleb Kichaev, Amir S Khan, Justin Aubin, Tracy S Zimmermann, Niranjan Y. Sardesai   +8 more
doaj   +1 more source

Systemic Optimization of Gene Electrotransfer Protocol Using Hard-to-Transfect UT-7 Cell Line as a Model

Biomedicines, 2022
Non-adherent cells are difficult to transfect with chemical-mediated delivery methods. Electroporation is an attractive strategy to transfer the molecules of interest into suspension cells.
Roberta Vadeikienė, Baltramiejus Jakštys, Rasa Ugenskienė, Saulius Šatkauskas, Elona Juozaitytė   +4 more
doaj   +1 more source

Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma

Mediators of Inflammation, 2017
Cancer immunotherapy is currently one of the leading approaches in cancer treatment. Gene electrotransfer of plasmids encoding interleukin 12 (IL-12) into the cells leads to the production of IL-12, which drives immune cell polarization to an antitumoral
Ursa Lampreht Tratar, Luisa Loiacono, Maja Cemazar, Urska Kamensek, Vito Michele Fazio, Gregor Sersa, Emanuela Signori   +6 more
doaj   +1 more source

Optimization of a gene electrotransfer procedure for efficient intradermal immunization with an hTERT-based DNA vaccine in mice

Molecular Therapy: Methods & Clinical Development, 2014
DNA vaccination consists in administering an antigen-encoding plasmid in order to trigger a specific immune response. This specific vaccine strategy is of particular interest to fight against various infectious diseases and cancer.
Christophe Y Calvet, Jessie Thalmensi, Christelle Liard, Elodie Pliquet, Thomas Bestetti, Thierry Huet, Pierre Langlade-Demoyen, Lluis M Mir   +7 more
doaj   +1 more source