Results 31 to 40 of about 5,598 (174)

Combined effect of heat and corona charge on molecular delivery to a T cell line in vitro.

PLoS ONE, 2023
With the rapid increase of gene and immunotherapies for treating cancer, there is a need to efficiently transfect cells. Previous studies suggest that electrotransfer can provide a non-viral method for gene delivery.
Molly A Skinner, Alex Otten, Andrew Hoff, Mark Jaroszeski   +3 more
doaj   +1 more source

Fast and non-viral method to deliver plasmid DNA to human muscle-tendon graft used in anterior cruciate ligament reconstruction

European Cells & Materials, 2023
Muscle tissues attached to the proximal ends of hamstring tendon grafts are routinely discarded during anterior cruciate ligament (ACL) reconstruction. Preserving the muscle on the ACL graft was shown to improve the surgery outcomes, possibly due to the ...
A Šećerović, B Markelc, I Matić, A Frischer, A Hacobian, T Jesenko, M Cemazar, H Redl, D Marolt Presen, A Ivković   +9 more
doaj   +1 more source

The Prodigious Potential of mRNA Electrotransfer as a Substitute to Conventional DNA-Based Transient Transfection

Cells, 2023
Transient transfection of foreign DNA is the most widely used laboratory technique to study gene function and product. However, the transfection efficiency depends on many parameters, including DNA quantity and quality, transfection methods and target ...
Théo Juncker, Bruno Chatton, Mariel Donzeau   +2 more
doaj   +1 more source

Electrotransfer of plasmid DNA radiosensitizes B16F10 tumors through activation of immune response

Radiology and Oncology, 2017
Tumor irradiation combined with adjuvant treatments, either vascular targeted or immunomodulatory, is under intense investigation. Gene electrotransfer of therapeutic genes is one of these approaches.
Savarin Monika, Kamensek Urska, Cemazar Maja, Heller Richard, Sersa Gregor   +4 more
doaj   +1 more source

An Integrated DNA Nanoprobe for Intranuclear Imaging and in Situ Profiling of OGG1 Activity

Advanced Science, EarlyView.
The TP‐SA nanoprobe, integrating an AS1411 aptamer for nuclear delivery and a FRET array for signal amplification, enables real‐time tracking of nuclear OGG1 activity. It reveals cell‐line‐specific basal OGG1 levels and shows clinical promise using pneumonia patients’ lavage fluid.
Mingzhu Zhao, Xuemei Sun, He Li, Bangming Wang, Mengting Pan, Zhen Ma, Rong‐Mei Kong, Weiheng Kong, Yan Zhao, Fengli Qu   +9 more
wiley   +1 more source

Hollow Cu2O Nanozymes Enhance Probiotic Therapy for Colitis via Redox Homeostasis and TXNIP/NLRP3 Inflammasome Inhibition

Advanced Science, EarlyView.
Hollow cuprous oxide (H‐Cu2O) nanozymes feature enlarged catalytic surfaces for superior reactive oxygen species (ROS) scavenging. By efficiently neutralizing mucosal ROS, H‐Cu2O directly suppresses the TXNIP/NLRP3 inflammasome axis and restores intestinal epithelial barrier integrity.
Guangzhao Wang, Jialing Cao, Pengfei Li, Yujie Wang, Shiqi Lu, Kangliang Sheng, Shan Gao, Yongzhong Wang   +7 more
wiley   +1 more source

Lanthanum Carbonate Nanosheets Based Colloidal Hydrogel for Modulating Innate & Adaptive Tumor Immunotherapy by Augmented Cellular Pyroptosis

Advanced Science, EarlyView.
The Gela/decitabine/La2(CO3)3 colloidal (GDLC) hydrogel developed here could reverse the epigenetic silencing of the GSDME gene by the synergistic interaction between DAC and La3+, while concurrently activating caspase‐3, thus facilitating pyroptosis induction under the tumor microenvironment.
Yang Hong, Haitao Wu, Weitao Wang, Xuan Zhang, Qian Chen, Yaoyu Hu, Zhengbao Zha   +6 more
wiley   +1 more source

Electroporator with automatic change of electric field direction improves gene electrotransfer in-vitro

BioMedical Engineering OnLine, 2007
Background Gene electrotransfer is a non-viral method used to transfer genes into living cells by means of high-voltage electric pulses. An exposure of a cell to an adequate amplitude and duration of electric pulses leads to a temporary increase of cell ...
Rols Marie-Pierre, Teissié Justin, Čorović Selma, Kandušer Maša, Faurie Cécile, Reberšek Matej, Miklavčič Damijan   +6 more
doaj   +1 more source

Thermal Assisted In Vivo Gene Electrotransfer

Current Gene Therapy, 2016
Gene electrotransfer is an effective approach for delivering plasmid DNA to a variety of tissues. Delivery of molecules with electric pulses requires control of the electrical parameters to achieve effective delivery. Since discomfort or tissue damage may occur with high applied voltage, the reduction of the applied voltage while achieving the desired ...
Donate, Amy, Bulysheva, Anna, Edelblute, Chelsea, Jung, Derrick, Malik, A., Quo, Siqi, Burcus, Niculina, Schoenbach, Karl, Heller, Richard   +8 more
openaire   +4 more sources

Biomaterial‐Integrated Electroporation for Therapeutic Delivery: From Gene Editing to Tumor Ablation and Immune Modulation

Small, EarlyView.
Biomaterial‐integrated electroporation establishes a programmable therapeutic interface that coordinates electric‐field modulation, transient or irreversible membrane permeabilization, cargo stabilization, and immune remodeling. This Review highlights how nanocarriers, hydrogels, and bioelectronic interfaces expand electroporation from conventional ...
Binbin Ji, Zhuozhuo Wu, Yuyue Jiang, Xiaoyu Liu, Jingfeng Li, Fengkuo Xu, Chaojie Li, Qungang Shan, Ziyin Wang, Junhao Wang, Wei Huang, Yaping Zhuang, Wenguo Cui, Zhongmin Wang   +13 more
wiley   +1 more source