Results 31 to 40 of about 5,729 (171)

Combined effect of heat and corona charge on molecular delivery to a T cell line in vitro.

PLoS ONE, 2023
With the rapid increase of gene and immunotherapies for treating cancer, there is a need to efficiently transfect cells. Previous studies suggest that electrotransfer can provide a non-viral method for gene delivery.
Molly A Skinner, Alex Otten, Andrew Hoff, Mark Jaroszeski   +3 more
doaj   +1 more source

In vivo DNA electrotransfer into muscle [PDF]

Development, Growth & Differentiation, 2008
Naked plasmid DNA injected into skeletal muscle is taken up by muscle cells and the genes in the plasmid are expressed. Among the non‐viral techniques for gene transfer in vivo, this method is especially simple, inexpensive, and safe. However, the relatively low expression levels attained by this method have limited its applications for uses other than
Satsuki, Miyazaki, Jun-Ichi, Miyazaki
openaire   +2 more sources

Fast and non-viral method to deliver plasmid DNA to human muscle-tendon graft used in anterior cruciate ligament reconstruction

European Cells & Materials, 2023
Muscle tissues attached to the proximal ends of hamstring tendon grafts are routinely discarded during anterior cruciate ligament (ACL) reconstruction. Preserving the muscle on the ACL graft was shown to improve the surgery outcomes, possibly due to the ...
A Šećerović, B Markelc, I Matić, A Frischer, A Hacobian, T Jesenko, M Cemazar, H Redl, D Marolt Presen, A Ivković   +9 more
doaj   +1 more source

Biomaterial-Integrated Electroporation for Therapeutic Delivery: From Gene Editing to Tumor Ablation and Immune Modulation. [PDF]

Small
Biomaterial‐integrated electroporation establishes a programmable therapeutic interface that coordinates electric‐field modulation, transient or irreversible membrane permeabilization, cargo stabilization, and immune remodeling. This Review highlights how nanocarriers, hydrogels, and bioelectronic interfaces expand electroporation from conventional ...
Ji B, Wu Z, Jiang Y, Liu X, Li J, Xu F, Li C, Shan Q, Wang Z, Wang J, Huang W, Zhuang Y, Cui W, Wang Z.   +13 more
europepmc   +2 more sources

The Prodigious Potential of mRNA Electrotransfer as a Substitute to Conventional DNA-Based Transient Transfection

Cells, 2023
Transient transfection of foreign DNA is the most widely used laboratory technique to study gene function and product. However, the transfection efficiency depends on many parameters, including DNA quantity and quality, transfection methods and target ...
Théo Juncker, Bruno Chatton, Mariel Donzeau   +2 more
doaj   +1 more source

Electrotransfer of plasmid DNA radiosensitizes B16F10 tumors through activation of immune response

Radiology and Oncology, 2017
Tumor irradiation combined with adjuvant treatments, either vascular targeted or immunomodulatory, is under intense investigation. Gene electrotransfer of therapeutic genes is one of these approaches.
Savarin Monika, Kamensek Urska, Cemazar Maja, Heller Richard, Sersa Gregor   +4 more
doaj   +1 more source

Detection of Mitochondrial Respiratory Chain Complexes by BN‐PAGE and Its Applications

Future Postharvest and Food, EarlyView.
Schematic workflow for the isolation and BN‐PAGE analysis of plant mitochondrial respiratory chain complexes. ABSTRACT Blue native polyacrylamide gel electrophoresis (BN‐PAGE) is a core technique for separating and characterizing plant mitochondrial respiratory chain complexes.
Jinghan Zhang, Jinyan Li, Hongliang Zhu
wiley   +1 more source

4′‐Hydroxywogonin Attenuates Hepatocellular Carcinoma Progression by Suppressing Gas6/Axl‐PI3K/AKT Signaling

Phytotherapy Research, EarlyView.
4′‐Hydroxywogonin, a flavonoid derived from Scutellaria baicalensis, inhibits hepatocellular carcinoma progression partly by suppressing Gas6/Axl‐PI3K/AKT signaling, inducing cell cycle arrest and apoptosis, and reducing metastatic potential. It also suppresses xenograft tumor growth without evident hepatotoxicity.
Chi‐Yuan Chen, Chin‐Chuan Chen, Chau‐Ting Yeh, Chieh‐Wen Chan, Yu‐De Chu, Yann‐Lii Leu, Roshan B. Satange, Li‐Fang Chou, Wen‐Yu Chuang, Chuen Hsueh, Tzong‐Ming Shieh, Tong‐Hong Wang   +11 more
wiley   +1 more source

Piezo1‐mediated unfolded protein response in goblet cells regulates intestinal mucus barrier homeostasis through the Ca2+–IRE1α–XBP1 pathway

Clinical and Translational Medicine, Volume 16, Issue 11, November 2026.
This study demonstrates that activation of Piezo1 channels in goblet cells by intestinal mechanical stimulation modulates ER calcium homeostasis via calcium ion (Ca2+) flux, which in turn regulates Mucin2 production and synthesis through the IRE1α‐XBP1. Intraperitoneal administration of Yoda1 alleviates mucus barrier impairment in DSS/WAS‐treated mice.
Jiandi Wu, Shaotong Wang, Zhe Wang, Jianhua Fang, Xueyan Li, Qingqing Zhang, Yan Dai, Wenjing Tian, Yize Ma, Gangping Li, Lei Zhang, Yudong Jiang, Xiaohua Hou, Jun Song   +13 more
wiley   +1 more source

Thermal Assisted In Vivo Gene Electrotransfer

Current Gene Therapy, 2016
Gene electrotransfer is an effective approach for delivering plasmid DNA to a variety of tissues. Delivery of molecules with electric pulses requires control of the electrical parameters to achieve effective delivery. Since discomfort or tissue damage may occur with high applied voltage, the reduction of the applied voltage while achieving the desired ...
Donate, Amy, Bulysheva, Anna, Edelblute, Chelsea, Jung, Derrick, Malik, A., Quo, Siqi, Burcus, Niculina, Schoenbach, Karl, Heller, Richard   +8 more
openaire   +4 more sources