Maintenance and gene electrotransfer efficiency of antibiotic resistance gene-free plasmids encoding mouse, canine and human interleukin-12 orthologues [PDF]
Heliyon, 2022 Interleukin 12 (IL-12) is a cytokine used as a therapeutic molecule in cancer immunotherapy. Gene electrotransfer mediated delivery of IL-12 gene has reached clinical evaluation in the USA using a plasmid that in addition to IL-12 gene also carry an ...
Urska Kamensek +5 more
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Interplay between Electric Field Strength and Number of Short-Duration Pulses for Efficient Gene Electrotransfer [PDF]
PharmaceuticalsElectroporation is a method that shows great promise as a non-viral approach for delivering genes by using high-voltage electric pulses to introduce DNA into cells to induce transient gene expression. This research aimed to evaluate the interplay between
Ernestas Urbanskas +6 more
doaj +4 more sources
Endocytosis and Endosomal Trafficking of DNA After Gene Electrotransfer In Vitro [PDF]
Molecular Therapy: Nucleic Acids, 2016 DNA electrotransfer is a successful technique for gene delivery into cells and represents an attractive alternative to virus-based methods for clinical applications including gene therapy and DNA vaccination.
Christelle Rosazza +5 more
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Gene Electrotransfer into Mammalian Cells Using Commercial Cell Culture Inserts with Porous Substrate [PDF]
Pharmaceutics, 2022 Gene electrotransfer is one of the main non-viral methods for intracellular delivery of plasmid DNA, wherein pulsed electric fields are used to transiently permeabilize the cell membrane, allowing enhanced transmembrane transport.
Tina Vindiš +5 more
doaj +2 more sources
The impact of impaired DNA mobility on gene electrotransfer efficiency: analysis in 3D model [PDF]
BioMedical Engineering OnLine, 2021 Background Gene electrotransfer is an established method that enables transfer of DNA into cells with electric pulses. Several studies analyzed and optimized different parameters of gene electrotransfer, however, one of main obstacles toward efficient ...
Saša Haberl Meglič, Mojca Pavlin
doaj +2 more sources
In Vivo and Ex Vivo Gene Electrotransfer in Ophthalmological Disorders [PDF]
Biomedicines, 2022 The aim of this document is to present an overview of gene electrotransfer in ophthalmological disorders. In order to ensure an adequate variety of the assessed studies, several electronic databases were considered and studies published between January ...
Roberta Fusco +6 more
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Systemic Optimization of Gene Electrotransfer Protocol Using Hard-to-Transfect UT-7 Cell Line as a Model [PDF]
Biomedicines, 2022 Non-adherent cells are difficult to transfect with chemical-mediated delivery methods. Electroporation is an attractive strategy to transfer the molecules of interest into suspension cells.
Roberta Vadeikienė +4 more
doaj +2 more sources
Electrotransfer parameters as a tool for controlled and targeted gene expression in skin
Molecular Therapy: Nucleic Acids, 2016 Skin is an attractive target for gene electrotransfer. It consists of different cell types that can be transfected, leading to various responses to gene electrotransfer. We demonstrate that these responses could be controlled by selecting the appropriate
Spela Kos +12 more
doaj +3 more sources
Applied Sciences, 2022 Electroporation (EP) allows for the transport of molecules into the cytoplasm with significant effectiveness by forming transient pores in the cell membrane using electric pulses. This can be used for cellular transport (RE—reversible electroporation) or
Katarzyna Rakoczy +9 more
doaj +3 more sources
Treatment of skin tumors with intratumoral interleukin 12 gene electrotransfer in the head and neck region: a first-in-human clinical trial protocol [PDF]
Radiology and Oncology, 2022 Immune therapies are currently under intensive investigation providing in many cases excellent responses in different tumors. Other possible approach for immunotherapy is a targeted intratumoral delivery of interleukin 12 (IL-12), a cytokine with anti ...
Groselj Ales +6 more
doaj +2 more sources