In Vivo and Ex Vivo Gene Electrotransfer in Ophthalmological Disorders [PDF]
Biomedicines, 2022 The aim of this document is to present an overview of gene electrotransfer in ophthalmological disorders. In order to ensure an adequate variety of the assessed studies, several electronic databases were considered and studies published between January ...
Roberta Fusco +6 more
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Endocytosis and Endosomal Trafficking of DNA After Gene Electrotransfer In Vitro [PDF]
Molecular Therapy: Nucleic Acids, 2016 DNA electrotransfer is a successful technique for gene delivery into cells and represents an attractive alternative to virus-based methods for clinical applications including gene therapy and DNA vaccination.
Christelle Rosazza +5 more
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Applied Sciences, 2022 Electroporation (EP) allows for the transport of molecules into the cytoplasm with significant effectiveness by forming transient pores in the cell membrane using electric pulses. This can be used for cellular transport (RE—reversible electroporation) or
Katarzyna Rakoczy +9 more
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Gene Electrotransfer into Mammalian Cells Using Commercial Cell Culture Inserts with Porous Substrate [PDF]
Pharmaceutics, 2022 Gene electrotransfer is one of the main non-viral methods for intracellular delivery of plasmid DNA, wherein pulsed electric fields are used to transiently permeabilize the cell membrane, allowing enhanced transmembrane transport.
Tina Vindiš +5 more
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Gene electrotransfer enhanced by nanosecond pulsed electric fields [PDF]
Molecular Therapy: Methods & Clinical Development, 2014 The impact of nanosecond pulsed electric fields (nsPEFs) on gene electrotransfer has not been clearly demonstrated in previous studies. This study was conducted to evaluate the influence of nsPEFs on the delivery of plasmids encoding luciferase or green ...
Siqi Guo +5 more
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Numerical optimization of gene electrotransfer into muscle tissue [PDF]
BioMedical Engineering OnLine, 2010 Background Electroporation-based gene therapy and DNA vaccination are promising medical applications that depend on transfer of pDNA into target tissues with use of electric pulses.
Miklavcic Damijan +3 more
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Maintenance and gene electrotransfer efficiency of antibiotic resistance gene-free plasmids encoding mouse, canine and human interleukin-12 orthologues [PDF]
Heliyon, 2022 Interleukin 12 (IL-12) is a cytokine used as a therapeutic molecule in cancer immunotherapy. Gene electrotransfer mediated delivery of IL-12 gene has reached clinical evaluation in the USA using a plasmid that in addition to IL-12 gene also carry an ...
Urska Kamensek +5 more
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Interplay between Electric Field Strength and Number of Short-Duration Pulses for Efficient Gene Electrotransfer [PDF]
PharmaceuticalsElectroporation is a method that shows great promise as a non-viral approach for delivering genes by using high-voltage electric pulses to introduce DNA into cells to induce transient gene expression. This research aimed to evaluate the interplay between
Ernestas Urbanskas +6 more
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The impact of impaired DNA mobility on gene electrotransfer efficiency: analysis in 3D model [PDF]
BioMedical Engineering OnLine, 2021 Background Gene electrotransfer is an established method that enables transfer of DNA into cells with electric pulses. Several studies analyzed and optimized different parameters of gene electrotransfer, however, one of main obstacles toward efficient ...
Saša Haberl Meglič, Mojca Pavlin
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Systemic Optimization of Gene Electrotransfer Protocol Using Hard-to-Transfect UT-7 Cell Line as a Model [PDF]
Biomedicines, 2022 Non-adherent cells are difficult to transfect with chemical-mediated delivery methods. Electroporation is an attractive strategy to transfer the molecules of interest into suspension cells.
Roberta Vadeikienė +4 more
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