Results 21 to 30 of about 2,697,950 (180)
Electrotransfer as a Non Viral Method of Gene Delivery
Current Gene Therapy, 2007 Over the last few decades, various vectors have been developed in the field of gene therapy. There still exist a number of important unresolved problems associated with the use of viral as well as non viral vectors. These techniques can suffer from secondary toxicity or low gene transfer efficiency.
Favard, Cyril +2 more
openaire +4 more sources
Engineering of a wireless single capacitive discharge conductivity-clamped gene electrotransfer probe for naked nucleic acid therapeutics [PDF]
APL BioengineeringPulsed electric field gene electrotransfer addresses the need for safe, directed “vector-free” delivery of DNA/mRNA therapeutics in deep tissue targets. We report the development of a disposable needle-type “electro-lens” probe for precision delivery of ...
Keng-Yin Lai +9 more
doaj +2 more sources
Vector‐Free Deep Tissue Targeting of DNA/RNA Therapeutics via Single Capacitive Discharge Conductivity‐Clamped Gene Electrotransfer [PDF]
Advanced ScienceViral vector and lipid nanoparticle based gene delivery have limitations around spatiotemporal control, transgene packaging size, and vector immune reactivity, compromising translation of nucleic acid (NA) therapeutics.
Jeremy L. Pinyon +13 more
doaj +2 more sources
Drug and Gene Electrotransfer in Cancer Therapy
, 2015 Drug and gene delivery by electroporation is gaining increasing momentum, in particular delivery of chemotherapeutic agents (electrochemotherapy) is now routinely used in many cancer centers, and gene therapy is in clinical trials. Gene therapy using electrotransfer offers the possibility to create a localized area hosting the transgene, with ...
Julie Gehl, Gehl, Julie
openaire +3 more sources
Gene Electrotransfer in 3D Reconstructed Human Dermal Tissue [PDF]
Current Gene Therapy, 2016 Gene electrotransfer into the skin is of particular interest for the development of medical applications including DNA vaccination, cancer treatment, wound healing or treatment of local skin disorders. However, such clinical applications are currently limited due to poor understanding of the mechanisms governing DNA electrotransfer within human tissue.
Madi, Moinecha +2 more
openaire +4 more sources
Gene electrotransfer enhanced by nanosecond pulsed electric fields
Molecular Therapy: Methods & Clinical Development, 2014 The impact of nanosecond pulsed electric fields (nsPEFs) on gene electrotransfer has not been clearly demonstrated in previous studies. This study was conducted to evaluate the influence of nsPEFs on the delivery of plasmids encoding luciferase or green ...
Siqi Guo +5 more
doaj +4 more sources
Gene Immunotherapy of Colon Carcinoma With IL-2 and IL-12 Using Gene Electrotransfer [PDF]
International Journal of Molecular Sciences, 2023 Masa Omerzel +2 more
exaly +2 more sources
In vivo gene expression: DNA electrotransfer.
Current opinion in molecular therapeutics, 2003 The use of electric pulses to deliver therapeutic molecules to tissues and organs in vivo is a rapidly growing field of research. Electrotransfer can be used to deliver a wide range of potentially therapeutic agents, including drugs, proteins, oligonucleotides, RNA and DNA.
Trezise, A. E. +3 more
openaire +4 more sources
Case Reports in Dermatology, 2022 We report a case of successful treatment of cutaneous metastases in HER2-positive breast cancer with calcium electroporation (CaEP), in addition to trastuzumab, over a period of 5 years.
Katrine Borres Jensen +3 more
doaj +1 more source
Gene Electrotransfer: A Mechanistic Perspective. [PDF]
Curr Gene Ther, 2016 Gene electrotransfer is a powerful method of DNA delivery offering several medical applications, among the most promising of which are DNA vaccination and gene therapy for cancer treatment. Electroporation entails the application of electric fields to cells which then experience a local and transient change of membrane permeability.
Rosazza C +4 more
europepmc +4 more sources