Numerical optimization of gene electrotransfer into muscle tissue [PDF]
BioMedical Engineering OnLine, 2010 Background Electroporation-based gene therapy and DNA vaccination are promising medical applications that depend on transfer of pDNA into target tissues with use of electric pulses.
Miklavcic Damijan +3 more
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Numerical study of gene electrotransfer efficiency based on electroporation volume and electrophoretic movement of plasmid DNA [PDF]
BioMedical Engineering OnLine, 2018 Background The efficiency of gene electrotransfer, an electroporation-based method for delivery of pDNA into target tissues, depends on several processes.
Tadeja Forjanič, Damijan Miklavčič
doaj +3 more sources
Effects of electrochemotherapy with cisplatin and peritumoral IL-12 gene electrotransfer on canine mast cell tumors: a histopathologic and immunohistochemical study [PDF]
Radiology and Oncology, 2017 The study was aimed to characterize tumor response after combined treatment employing electrochemotherapy with IL-12 gene electrotransfer in dogs with spontaneous mast cell tumors (MCT).
Salvadori Claudia +9 more
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Topical gene electrotransfer to the epidermis of hairless guinea pig by non-invasive multielectrode array. [PDF]
PLoS ONE, 2013 Topical gene delivery to the epidermis has the potential to be an effective therapy for skin disorders, cutaneous cancers, vaccinations and systemic metabolic diseases. Previously, we reported on a non-invasive multielectrode array (MEA) that efficiently
Siqi Guo +4 more
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Acute Effects of Intratumor DNA Electrotransfer
Pharmaceutics, 2022 Intratumor therapeutic DNA electroporation or electrotransfer is in clinical trials in the United States and is under development in many other countries.
Manya Bhandary +3 more
doaj +2 more sources
Thermal Assisted In Vivo Gene Electrotransfer. [PDF]
Curr Gene Ther, 2016 Gene electrotransfer is an effective approach for delivering plasmid DNA to a variety of tissues. Delivery of molecules with electric pulses requires control of the electrical parameters to achieve effective delivery. Since discomfort or tissue damage may occur with high applied voltage, the reduction of the applied voltage while achieving the desired ...
Donate A +8 more
europepmc +7 more sources
Muscle gene electrotransfer is increased by the antioxidant tempol in mice [PDF]
Gene Therapy, 2011 Electropermeabilization (EP) is an effective method of gene transfer into different tissues. During EP, reactive oxygen species (ROS) are formed, which could affect transfection efficiency. The role of generated ROS and the role of antioxidants in electrotransfer in myoblasts in vitro and in Musculus tibialis cranialis in mice were, therefore ...
Markelc, B. +7 more
openaire +7 more sources
Noninvasive Gene Electrotransfer in Skin [PDF]
Human Gene Therapy Methods, 2019 The skin is considered as well suited for gene therapy and vaccination. DNA vaccines elicit both broad humoral and cellular immune responses when injected in the skin. Physical and chemical methods are needed to boost the expression. Gene electrotransfer (GET) is one of the most effective approaches.
Lise, Pasquet +5 more
openaire +3 more sources
Radiosensitizing effect of intratumoral interleukin-12 gene electrotransfer in murine sarcoma
BMC Cancer, 2013 Background Interleukin-12 (IL-12) based radiosensitization is an effective way of tumor treatment. Local cytokine production, without systemic shedding, might provide clinical benefit in radiation treatment of sarcomas.
Sedlar Ales +5 more
doaj +2 more sources
Mediators of Inflammation, 2017 Cancer immunotherapy is currently one of the leading approaches in cancer treatment. Gene electrotransfer of plasmids encoding interleukin 12 (IL-12) into the cells leads to the production of IL-12, which drives immune cell polarization to an antitumoral
Ursa Lampreht Tratar +6 more
doaj +2 more sources