Results 11 to 20 of about 2,519,482 (174)

Thermal Assisted In Vivo Gene Electrotransfer. [PDF]

Curr Gene Ther, 2016
Gene electrotransfer is an effective approach for delivering plasmid DNA to a variety of tissues. Delivery of molecules with electric pulses requires control of the electrical parameters to achieve effective delivery. Since discomfort or tissue damage may occur with high applied voltage, the reduction of the applied voltage while achieving the desired ...
Donate A, Bulysheva A, Edelblute C, Jung D, Malik MA, Guo S, Burcus N, Schoenbach K, Heller R.   +8 more
europepmc   +7 more sources

Electrotransfer parameters as a tool for controlled and targeted gene expression in skin

Molecular Therapy: Nucleic Acids, 2016
Skin is an attractive target for gene electrotransfer. It consists of different cell types that can be transfected, leading to various responses to gene electrotransfer. We demonstrate that these responses could be controlled by selecting the appropriate
Spela Kos, Tanja Blagus, Maja Cemazar, Ursa Lampreht Tratar, Monika Stimac, Lara Prosen, Tanja Dolinsek, Urska Kamensek, Simona Kranjc, Lars Steinstraesser, Gaëlle Vandermeulen, Véronique Préat, Gregor Sersa   +12 more
doaj   +4 more sources

Acute Effects of Intratumor DNA Electrotransfer

Pharmaceutics, 2022
Intratumor therapeutic DNA electroporation or electrotransfer is in clinical trials in the United States and is under development in many other countries.
Manya Bhandary, Amanda Sales Conniff, Kaitlyn Miranda, Loree C. Heller   +3 more
doaj   +2 more sources

Topical gene electrotransfer to the epidermis of hairless guinea pig by non-invasive multielectrode array. [PDF]

PLoS ONE, 2013
Topical gene delivery to the epidermis has the potential to be an effective therapy for skin disorders, cutaneous cancers, vaccinations and systemic metabolic diseases. Previously, we reported on a non-invasive multielectrode array (MEA) that efficiently
Siqi Guo, Annelise L Israel, Gaurav Basu, Amy Donate, Richard Heller   +4 more
doaj   +3 more sources

Gene electrotransfer: Potential for gene therapy of renal diseases [PDF]

Kidney International, 2002
The ability to pursue gene therapy has been limited by the availability of an effective and safe system for gene delivery, especially to the kidney. Electroporation is an efficient method to transfer physiologically the gene to the cells without complicated preparation.
Imai, Enyu, Isaka, Yoshitaka
openaire   +4 more sources

Noninvasive Gene Electrotransfer in Skin [PDF]

Human Gene Therapy Methods, 2019
The skin is considered as well suited for gene therapy and vaccination. DNA vaccines elicit both broad humoral and cellular immune responses when injected in the skin. Physical and chemical methods are needed to boost the expression. Gene electrotransfer (GET) is one of the most effective approaches.
Lise, Pasquet, Sophie, Chabot, Elisabeth, Bellard, Marie-Pierre, Rols, Justin, Teissie, Muriel, Golzio   +5 more
openaire   +3 more sources

Muscle gene electrotransfer is increased by the antioxidant tempol in mice [PDF]

Gene Therapy, 2011
Electropermeabilization (EP) is an effective method of gene transfer into different tissues. During EP, reactive oxygen species (ROS) are formed, which could affect transfection efficiency. The role of generated ROS and the role of antioxidants in electrotransfer in myoblasts in vitro and in Musculus tibialis cranialis in mice were, therefore ...
Markelc, B., Tevz, Gregor, Cemazar, Maja, Kranjc, Simona, Lavrencak, J., Zegura, B., Teissie, J., Sersa, Gregor   +7 more
openaire   +7 more sources

Radiosensitizing effect of intratumoral interleukin-12 gene electrotransfer in murine sarcoma

BMC Cancer, 2013
Background Interleukin-12 (IL-12) based radiosensitization is an effective way of tumor treatment. Local cytokine production, without systemic shedding, might provide clinical benefit in radiation treatment of sarcomas.
Sedlar Ales, Kranjc Simona, Dolinsek Tanja, Cemazar Maja, Coer Andrej, Sersa Gregor   +5 more
doaj   +2 more sources

Gene Electrotransfer: A Mechanistic Perspective. [PDF]

Curr Gene Ther, 2016
Gene electrotransfer is a powerful method of DNA delivery offering several medical applications, among the most promising of which are DNA vaccination and gene therapy for cancer treatment. Electroporation entails the application of electric fields to cells which then experience a local and transient change of membrane permeability.
Rosazza C, Meglic SH, Zumbusch A, Rols MP, Miklavcic D.   +4 more
europepmc   +4 more sources

Gene Electrotransfer in 3D Reconstructed Human Dermal Tissue [PDF]

Current Gene Therapy, 2016
Gene electrotransfer into the skin is of particular interest for the development of medical applications including DNA vaccination, cancer treatment, wound healing or treatment of local skin disorders. However, such clinical applications are currently limited due to poor understanding of the mechanisms governing DNA electrotransfer within human tissue.
Madi, Moinecha, Rols, Marie-Pierre, Gibot, Laure   +2 more
openaire   +4 more sources