Results 31 to 40 of about 2,697,950 (180)
Calcium Electroporation of Equine Sarcoids
Animals, 2020 Sarcoids are common equine skin tumors where the risk of recurrence after treatment is high, and better treatment options are warranted. Calcium electroporation is a novel anti-cancer treatment where lethally high calcium concentrations are introduced ...
Stine K. Frandsen +3 more
doaj +1 more source
The Influence of Calcium Ions on the Electrotransfer Efficiency of Plasmid DNA and Cell Viability
Applied Sciences, 2023 Gene electrotransfer (GET) is recognized as a promising technique for the development of an efficient tool for gene therapy. Such a therapy would have applications in the treatment of a variety of genetic diseases, including cancer.
Rūta Palepšienė +4 more
doaj +1 more source
Between defence and delivery: the DNA sensing response to gene electrotransfer. [PDF]
Radiol OncolAbstract Background Gene therapy has emerged as a transformative biomedical approach, offering new therapeutic possibilities from many so far uncurable diseases through the introduction of recombinant nucleic acids into target cells.
Jesenko T +3 more
europepmc +4 more sources
Gene electrotransfer: Potential for gene therapy of renal diseases [PDF]
Kidney International, 2002 The ability to pursue gene therapy has been limited by the availability of an effective and safe system for gene delivery, especially to the kidney. Electroporation is an efficient method to transfer physiologically the gene to the cells without complicated preparation.
Imai, Enyu, Isaka, Yoshitaka
openaire +2 more sources
IL-12 Gene Electrotransfer Triggers a Change in Immune Response within Mouse Tumors [PDF]
Cancers, 2018 Richard Heller +2 more
exaly +2 more sources
Molecular Therapy: Methods & Clinical Development, 2014 DNA vaccination consists in administering an antigen-encoding plasmid in order to trigger a specific immune response. This specific vaccine strategy is of particular interest to fight against various infectious diseases and cancer.
Christophe Y Calvet +7 more
doaj +1 more source
European Cells & Materials, 2023 Muscle tissues attached to the proximal ends of hamstring tendon grafts are routinely discarded during anterior cruciate ligament (ACL) reconstruction. Preserving the muscle on the ACL graft was shown to improve the surgery outcomes, possibly due to the ...
A Šećerović +9 more
doaj +1 more source
Gene expression profiles in skeletal muscle after gene electrotransfer [PDF]
BMC Molecular Biology, 2007 Gene transfer by electroporation (DNA electrotransfer) to muscle results in high level long term transgenic expression, showing great promise for treatment of e.g. protein deficiency syndromes. However little is known about the effects of DNA electrotransfer on muscle fibres.
Hojman, Pernille +4 more
openaire +3 more sources
Overcoming the Specific Toxicity of Large Plasmids Electrotransfer in Primary Cells In Vitro
Molecular Therapy: Nucleic Acids, 2016 Gene electrotransfer is a safe and efficient nonviral technique for the transfer of nucleic acids of all sizes. Using a small reporter plasmid (3.5 kbp), electrotransfer of more than 90% of the cells, with ≃70% viability, can be routinely achieved even ...
Léa L Lesueur +2 more
doaj +1 more source
Combined effect of heat and corona charge on molecular delivery to a T cell line in vitro.
PLoS ONE, 2023 With the rapid increase of gene and immunotherapies for treating cancer, there is a need to efficiently transfect cells. Previous studies suggest that electrotransfer can provide a non-viral method for gene delivery.
Molly A Skinner +3 more
doaj +1 more source