Results 31 to 40 of about 4,599 (157)

Fast and non-viral method to deliver plasmid DNA to human muscle-tendon graft used in anterior cruciate ligament reconstruction

European Cells & Materials, 2023
Muscle tissues attached to the proximal ends of hamstring tendon grafts are routinely discarded during anterior cruciate ligament (ACL) reconstruction. Preserving the muscle on the ACL graft was shown to improve the surgery outcomes, possibly due to the ...
A Šećerović, B Markelc, I Matić, A Frischer, A Hacobian, T Jesenko, M Cemazar, H Redl, D Marolt Presen, A Ivković   +9 more
doaj   +1 more source

Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma

Mediators of Inflammation, 2017
Cancer immunotherapy is currently one of the leading approaches in cancer treatment. Gene electrotransfer of plasmids encoding interleukin 12 (IL-12) into the cells leads to the production of IL-12, which drives immune cell polarization to an antitumoral
Ursa Lampreht Tratar, Luisa Loiacono, Maja Cemazar, Urska Kamensek, Vito Michele Fazio, Gregor Sersa, Emanuela Signori   +6 more
doaj   +1 more source

Overcoming the Specific Toxicity of Large Plasmids Electrotransfer in Primary Cells In Vitro

Molecular Therapy: Nucleic Acids, 2016
Gene electrotransfer is a safe and efficient nonviral technique for the transfer of nucleic acids of all sizes. Using a small reporter plasmid (3.5 kbp), electrotransfer of more than 90% of the cells, with ≃70% viability, can be routinely achieved even ...
Léa L Lesueur, Lluis M Mir, Franck M André   +2 more
doaj   +1 more source

Combined effect of heat and corona charge on molecular delivery to a T cell line in vitro.

PLoS ONE, 2023
With the rapid increase of gene and immunotherapies for treating cancer, there is a need to efficiently transfect cells. Previous studies suggest that electrotransfer can provide a non-viral method for gene delivery.
Molly A Skinner, Alex Otten, Andrew Hoff, Mark Jaroszeski   +3 more
doaj   +1 more source

Gene expression profiles in skeletal muscle after gene electrotransfer [PDF]

BMC Molecular Biology, 2007
Gene transfer by electroporation (DNA electrotransfer) to muscle results in high level long term transgenic expression, showing great promise for treatment of e.g. protein deficiency syndromes. However little is known about the effects of DNA electrotransfer on muscle fibres.
Hojman, Pernille, Zibert, John R, Gissel, Hanne, Eriksen, Jens, Gehl, Julie   +4 more
openaire   +3 more sources

ELECTROTRANSFER OF CYTOKINE GENES FOR CANCER TREATMENT

CBU International Conference Proceedings, 2018
Gene electrotransfer, which designates the combination of gene transfer and electroporation, is a physical method for transfecting genes into cells and tissues. Many reports for the utilization of this techniques in animals confirmed that gene electrotransfer is a safe and efficient method.
Chopra, Sonam, Šatkauskas, Saulius
openaire   +2 more sources

Muscle gene electrotransfer is increased by the antioxidant tempol in mice [PDF]

Gene Therapy, 2011
Electropermeabilization (EP) is an effective method of gene transfer into different tissues. During EP, reactive oxygen species (ROS) are formed, which could affect transfection efficiency. The role of generated ROS and the role of antioxidants in electrotransfer in myoblasts in vitro and in Musculus tibialis cranialis in mice were, therefore ...
Markelc, B., Tevz, Gregor, Cemazar, Maja, Kranjc, Simona, Lavrencak, J., Zegura, B., Teissie, J., Sersa, Gregor   +7 more
openaire   +5 more sources

In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle Tissue

Biological Procedures Online, 2009
DNA electrotransfer to muscle tissue yields long-term, high levels of gene expression; showing great promise for future gene therapy. We want to characterize the novel far-red fluorescent protein Katushka as a marker for gene expression using time domain
Eriksen Jens, Gehl Julie, Hojman Pernille   +2 more
doaj   +3 more sources

Electroporator with automatic change of electric field direction improves gene electrotransfer in-vitro

BioMedical Engineering OnLine, 2007
Background Gene electrotransfer is a non-viral method used to transfer genes into living cells by means of high-voltage electric pulses. An exposure of a cell to an adequate amplitude and duration of electric pulses leads to a temporary increase of cell ...
Rols Marie-Pierre, Teissié Justin, Čorović Selma, Kandušer Maša, Faurie Cécile, Reberšek Matej, Miklavčič Damijan   +6 more
doaj   +1 more source

Immune cell populations in the tumour environment following calcium electropora­tion for cutaneous metastasis: a histopathological study

Acta Oncologica
Background and Purpose: Calcium electroporation (CaEP) involves injecting calcium into tumour tissues and using electrical pulses to create membrane pores that induce cell death.
Mille Vissing, Sandra Sinius Pouplier, Lars Munch Larsen, Stine Krog Frandsen, Alexey Lodin, Anne-Vibeke Lænkholm, Julie Gehl   +6 more
doaj   +1 more source