Results 51 to 60 of about 2,519,482 (174)

The Prodigious Potential of mRNA Electrotransfer as a Substitute to Conventional DNA-Based Transient Transfection

Cells, 2023
Transient transfection of foreign DNA is the most widely used laboratory technique to study gene function and product. However, the transfection efficiency depends on many parameters, including DNA quantity and quality, transfection methods and target ...
Théo Juncker, Bruno Chatton, Mariel Donzeau   +2 more
doaj   +1 more source

pFAR plasmids: New Eukaryotic Expression Vectors for Gene Therapy, devoid of Antibiotic Resistance Markers [PDF]

, 2008
Efficient production of eukaryotic expression vectors requires the selection of plasmid-containing bacteria. To avoid the risk of dissemination of antibiotic resistance markers, we developed a new system to produce a family of plasmids Free of Antibiotic
Corinne Marie, Daniel Scherman, Veronique Preat, Gaelle Vandermeulen, Mickael Quiviger, Magali Richard   +5 more
core  

Hollow Cu2O Nanozymes Enhance Probiotic Therapy for Colitis via Redox Homeostasis and TXNIP/NLRP3 Inflammasome Inhibition

Advanced Science, EarlyView.
Hollow cuprous oxide (H‐Cu2O) nanozymes feature enlarged catalytic surfaces for superior reactive oxygen species (ROS) scavenging. By efficiently neutralizing mucosal ROS, H‐Cu2O directly suppresses the TXNIP/NLRP3 inflammasome axis and restores intestinal epithelial barrier integrity.
Guangzhao Wang, Jialing Cao, Pengfei Li, Yujie Wang, Shiqi Lu, Kangliang Sheng, Shan Gao, Yongzhong Wang   +7 more
wiley   +1 more source

Lanthanum Carbonate Nanosheets Based Colloidal Hydrogel for Modulating Innate & Adaptive Tumor Immunotherapy by Augmented Cellular Pyroptosis

Advanced Science, EarlyView.
The Gela/decitabine/La2(CO3)3 colloidal (GDLC) hydrogel developed here could reverse the epigenetic silencing of the GSDME gene by the synergistic interaction between DAC and La3+, while concurrently activating caspase‐3, thus facilitating pyroptosis induction under the tumor microenvironment.
Yang Hong, Haitao Wu, Weitao Wang, Xuan Zhang, Qian Chen, Yaoyu Hu, Zhengbao Zha   +6 more
wiley   +1 more source

Influence of DNA characteristics on cell membrane damage stimulated by electrical short-circuiting via a low-conductive aqueous droplet in dielectric oil.

PLoS ONE, 2023
We investigated gene electrotransfer using electrical short-circuiting via a cell suspension droplet in dielectric oil. An aqueous droplet of a few microliters placed between a pair of electrodes can be deformed by an intense DC electric field depending ...
Yoshino Tsurusaki, Yuki Watanabe, Rika Numano, Takayuki Shibata, Hirofumi Kurita   +4 more
doaj   +1 more source

Gene Editing of Nicotiana benthamiana Architecture for Space‐Efficient Production of Recombinant Proteins in Closed Environments

Plant Biotechnology Journal, EarlyView.
ABSTRACT Indoor vertical farming (VF) offers practical advantages for the cultivation of plant protein bio‐factories including plant uniformity, product consistency, water/nutrient recycling and production cycles on a year‐round basis. Much progress has been achieved toward the development of innovative systems for artificial lighting, automated ...
Béatrice Giroux, Kristina LeBreux, Louis Feyzeau, Marie‐Claire Goulet, Charles Goulet, Dominique Michaud   +5 more
wiley   +1 more source

Electrotransfer of siRNA to Silence Enhanced Green Fluorescent Protein in Tumor Mediated by a High Intensity Pulsed Electromagnetic Field

Vaccines, 2020
The contactless high intensity pulsed electromagnetic field (HI-PEMF)-induced increase of cell membrane permeability is similar to conventional electroporation, with the important difference of inducing an electric field non-invasively by exposing a ...
Simona Kranjc Brezar, Matej Kranjc, Maja Čemažar, Simon Buček, Gregor Serša, Damijan Miklavčič   +5 more
doaj   +1 more source

Mapping of bionic array electric field focusing in plasmid DNA-based gene electrotransfer. [PDF]

Gene Ther, 2016
Molecular medicine through gene therapy is challenged to achieve targeted action. This is now possible utilizing bionic electrode arrays for focal delivery of naked (plasmid) DNA via gene electrotransfer.
Browne CJ, Pinyon JL, Housley DM, Crawford EN, Lovell NH, Klugmann M, Housley GD.   +6 more
europepmc   +2 more sources

The ApHSF–ApCtf1β2–ApCUT3 Cascade Couples Host ROS Detoxification to Cuticle Penetration During Fungal Pathogenesis

Molecular Plant Pathology, Volume 27, Issue 9, September 2026.
The ApHSF–ApCtf1β2–ApCUT3 transcriptional cascade links fungal adaptation to host‐derived ROS with cuticle penetration during infection. ApHSF promotes ROS detoxification and activates ApCtf1β2, which induces ApCUT3 expression and cuticle degradation, thereby coordinating oxidative stress adaptation with host barrier penetration.
Peng Yan, Lin Li, Sijia Liu, Yutong Liu, Zhanbo Liu, Han Zhao, Shujiang Li   +6 more
wiley   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, Volume 16, Issue 8, Page 1491-1500, August 2026.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source