Results 51 to 60 of about 2,697,950 (180)

pFAR plasmids: New Eukaryotic Expression Vectors for Gene Therapy, devoid of Antibiotic Resistance Markers [PDF]

, 2008
Efficient production of eukaryotic expression vectors requires the selection of plasmid-containing bacteria. To avoid the risk of dissemination of antibiotic resistance markers, we developed a new system to produce a family of plasmids Free of Antibiotic
Corinne Marie, Daniel Scherman, Veronique Preat, Gaelle Vandermeulen, Mickael Quiviger, Magali Richard   +5 more
core  

4′‐Hydroxywogonin Attenuates Hepatocellular Carcinoma Progression by Suppressing Gas6/Axl‐PI3K/AKT Signaling

Phytotherapy Research, EarlyView.
4′‐Hydroxywogonin, a flavonoid derived from Scutellaria baicalensis, inhibits hepatocellular carcinoma progression partly by suppressing Gas6/Axl‐PI3K/AKT signaling, inducing cell cycle arrest and apoptosis, and reducing metastatic potential. It also suppresses xenograft tumor growth without evident hepatotoxicity.
Chi‐Yuan Chen, Chin‐Chuan Chen, Chau‐Ting Yeh, Chieh‐Wen Chan, Yu‐De Chu, Yann‐Lii Leu, Roshan B. Satange, Li‐Fang Chou, Wen‐Yu Chuang, Chuen Hsueh, Tzong‐Ming Shieh, Tong‐Hong Wang   +11 more
wiley   +1 more source

Influence of DNA characteristics on cell membrane damage stimulated by electrical short-circuiting via a low-conductive aqueous droplet in dielectric oil.

PLoS ONE, 2023
We investigated gene electrotransfer using electrical short-circuiting via a cell suspension droplet in dielectric oil. An aqueous droplet of a few microliters placed between a pair of electrodes can be deformed by an intense DC electric field depending ...
Yoshino Tsurusaki, Yuki Watanabe, Rika Numano, Takayuki Shibata, Hirofumi Kurita   +4 more
doaj   +1 more source

Gene Editing of Nicotiana benthamiana Architecture for Space‐Efficient Production of Recombinant Proteins in Closed Environments

Plant Biotechnology Journal, EarlyView.
ABSTRACT Indoor vertical farming (VF) offers practical advantages for the cultivation of plant protein bio‐factories including plant uniformity, product consistency, water/nutrient recycling and production cycles on a year‐round basis. Much progress has been achieved toward the development of innovative systems for artificial lighting, automated ...
Béatrice Giroux, Kristina LeBreux, Louis Feyzeau, Marie‐Claire Goulet, Charles Goulet, Dominique Michaud   +5 more
wiley   +1 more source

Electrotransfer of Plasmid DNA

, 2011
International audienceVarious vectors have been developed in the field of gene transfer. However, important unresolvedproblems persist such as secondary toxicity or low gene transfer efficiency of viral as well as nonviral vectors.
Jean-Michel Escoffre, Dean, David, Marie-Pierre Rols, Rols, Marie-Pierre, David A. Dean, Escoffre, Jean-Michel   +5 more
core   +1 more source

Electrotransfer of siRNA to Silence Enhanced Green Fluorescent Protein in Tumor Mediated by a High Intensity Pulsed Electromagnetic Field

Vaccines, 2020
The contactless high intensity pulsed electromagnetic field (HI-PEMF)-induced increase of cell membrane permeability is similar to conventional electroporation, with the important difference of inducing an electric field non-invasively by exposing a ...
Simona Kranjc Brezar, Matej Kranjc, Maja Čemažar, Simon Buček, Gregor Serša, Damijan Miklavčič   +5 more
doaj   +1 more source

Piezo1‐mediated unfolded protein response in goblet cells regulates intestinal mucus barrier homeostasis through the Ca2+–IRE1α–XBP1 pathway

Clinical and Translational Medicine, Volume 16, Issue 11, November 2026.
This study demonstrates that activation of Piezo1 channels in goblet cells by intestinal mechanical stimulation modulates ER calcium homeostasis via calcium ion (Ca2+) flux, which in turn regulates Mucin2 production and synthesis through the IRE1α‐XBP1. Intraperitoneal administration of Yoda1 alleviates mucus barrier impairment in DSS/WAS‐treated mice.
Jiandi Wu, Shaotong Wang, Zhe Wang, Jianhua Fang, Xueyan Li, Qingqing Zhang, Yan Dai, Wenjing Tian, Yize Ma, Gangping Li, Lei Zhang, Yudong Jiang, Xiaohua Hou, Jun Song   +13 more
wiley   +1 more source

Hollow Cu2O Nanozymes Enhance Probiotic Therapy for Colitis via Redox Homeostasis and TXNIP/NLRP3 Inflammasome Inhibition

Advanced Science, Volume 13, Issue 56, 9 October 2026.
Hollow cuprous oxide (H‐Cu2O) nanozymes feature enlarged catalytic surfaces for superior reactive oxygen species (ROS) scavenging. By efficiently neutralizing mucosal ROS, H‐Cu2O directly suppresses the TXNIP/NLRP3 inflammasome axis and restores intestinal epithelial barrier integrity.
Guangzhao Wang, Jialing Cao, Pengfei Li, Yujie Wang, Shiqi Lu, Kangliang Sheng, Shan Gao, Yongzhong Wang   +7 more
wiley   +1 more source

Mcam Silencing With RNA Interference Using Magnetofection has Antitumor Effect in Murine Melanoma

Molecular Therapy: Nucleic Acids, 2014
The melanoma cell adhesion molecule (MCAM) is involved in melanoma development and its progression, including invasiveness, metastatic potential and angiogenesis.
Lara Prosen, Bostjan Markelc, Tanja Dolinsek, Branka Music, Maja Cemazar, Gregor Sersa   +5 more
doaj   +1 more source

Lanthanum Carbonate Nanosheets Based Colloidal Hydrogel for Modulating Innate & Adaptive Tumor Immunotherapy by Augmented Cellular Pyroptosis

Advanced Science, Volume 13, Issue 60, 28 October 2026.
The Gela/decitabine/La2(CO3)3 colloidal (GDLC) hydrogel developed here could reverse the epigenetic silencing of the GSDME gene by the synergistic interaction between DAC and La3+, while concurrently activating caspase‐3, thus facilitating pyroptosis induction under the tumor microenvironment.
Yang Hong, Haitao Wu, Weitao Wang, Xuan Zhang, Qian Chen, Yaoyu Hu, Zhengbao Zha   +6 more
wiley   +1 more source