Results 41 to 50 of about 5,729 (171)

Calcium Electrotransfer for Termination of Transgene Expression in Muscle [PDF]

Human Gene Therapy, 2011
Gene electrotransfer is expanding in clinical use, thus we have searched for an emergency procedure to stop transgene expression in case of serious adverse events. Calcium is cytotoxic at high intracellular levels, so we tested effects of calcium electrotransfer on transgene expression in muscle.
Hojman, Pernille, Spanggaard, Iben, Olsen, Caroline Holkman, Gehl, Julie, Gissel, Hanne   +4 more
openaire   +3 more sources

Electroporator with automatic change of electric field direction improves gene electrotransfer in-vitro

BioMedical Engineering OnLine, 2007
Background Gene electrotransfer is a non-viral method used to transfer genes into living cells by means of high-voltage electric pulses. An exposure of a cell to an adequate amplitude and duration of electric pulses leads to a temporary increase of cell ...
Rols Marie-Pierre, Teissié Justin, Čorović Selma, Kandušer Maša, Faurie Cécile, Reberšek Matej, Miklavčič Damijan   +6 more
doaj   +1 more source

Serpin A1 Cargo Within Tumour‐Derived Extracellular Vesicles Supports Pro‐Tumoural Bias of Neutrophils in Cancer

Journal of Extracellular Vesicles, Volume 15, Issue 11, November 2026.
ABSTRACT Tumour‐derived extracellular vesicles (EVs) or exosomes are critical mediators of the cancer‐immune system crosstalk, yet the mechanisms by which they reprogram neutrophil function in vivo remain insufficiently characterised. Here, we identify the serine protease inhibitor Serpin A1 as a predominant cargo component of small EVs (sEVs) released
Maksim Domnich, Ekaterina Pylaeva, Elena Siakaeva, Nastassia Kabankova, Agnieszka Bedzinska, Damian Sojka, Aneta Zebrowska, Marta Gawin, Maren Soldierer, Yaping Yu, Malwina Rist, Daniel Fochtman, Bernd Giebel, Iris Helfrich, Ilona Thiel, Basant Kumar Thakur, Cornelius H. L. Kürten, Helmut Hanenberg, Sonja Ludwig, Piotr Widlak, Stephan Lang, Monika Pietrowska, Jadwiga Jablonska   +22 more
wiley   +1 more source

Gene Editing of Nicotiana benthamiana Architecture for Space‐Efficient Production of Recombinant Proteins in Closed Environments

Plant Biotechnology Journal, Volume 24, Issue 11, Page 5600-5615, November 2026.
ABSTRACT Indoor vertical farming (VF) offers practical advantages for the cultivation of plant protein bio‐factories including plant uniformity, product consistency, water/nutrient recycling and production cycles on a year‐round basis. Much progress has been achieved toward the development of innovative systems for artificial lighting, automated ...
Béatrice Giroux, Kristina LeBreux, Louis Feyzeau, Marie‐Claire Goulet, Charles Goulet, Dominique Michaud   +5 more
wiley   +1 more source

Numerical study of gene electrotransfer efficiency based on electroporation volume and electrophoretic movement of plasmid DNA

BioMedical Engineering OnLine, 2018
Background The efficiency of gene electrotransfer, an electroporation-based method for delivery of pDNA into target tissues, depends on several processes.
Tadeja Forjanič, Damijan Miklavčič
doaj   +1 more source

In Vivo and Ex Vivo Gene Electrotransfer in Ophthalmological Disorders

Biomedicines, 2022
The aim of this document is to present an overview of gene electrotransfer in ophthalmological disorders. In order to ensure an adequate variety of the assessed studies, several electronic databases were considered and studies published between January ...
Roberta Fusco, Giacomo Perazzolo Gallo, Elio Di Bernardo, Valeria D’Alessio, Mattia Ronchetti, Matteo Cadossi, Ruggero Cadossi   +6 more
doaj   +1 more source

Hollow Cu2O Nanozymes Enhance Probiotic Therapy for Colitis via Redox Homeostasis and TXNIP/NLRP3 Inflammasome Inhibition

Advanced Science, Volume 13, Issue 56, 9 October 2026.
Hollow cuprous oxide (H‐Cu2O) nanozymes feature enlarged catalytic surfaces for superior reactive oxygen species (ROS) scavenging. By efficiently neutralizing mucosal ROS, H‐Cu2O directly suppresses the TXNIP/NLRP3 inflammasome axis and restores intestinal epithelial barrier integrity.
Guangzhao Wang, Jialing Cao, Pengfei Li, Yujie Wang, Shiqi Lu, Kangliang Sheng, Shan Gao, Yongzhong Wang   +7 more
wiley   +1 more source

The Evidence of the Bystander Effect after Bleomycin Electrotransfer and Irreversible Electroporation

Molecules, 2021
One of current applications of electroporation is electrochemotherapy and electroablation for local cancer treatment. Both of these electroporation modalities share some similarities with radiation therapy, one of which could be the bystander effect.
Paulius Ruzgys, Neringa Barauskaitė, Vitalij Novickij, Jurij Novickij, Saulius Šatkauskas   +4 more
doaj   +1 more source

Lanthanum Carbonate Nanosheets Based Colloidal Hydrogel for Modulating Innate & Adaptive Tumor Immunotherapy by Augmented Cellular Pyroptosis

Advanced Science, Volume 13, Issue 60, 28 October 2026.
The Gela/decitabine/La2(CO3)3 colloidal (GDLC) hydrogel developed here could reverse the epigenetic silencing of the GSDME gene by the synergistic interaction between DAC and La3+, while concurrently activating caspase‐3, thus facilitating pyroptosis induction under the tumor microenvironment.
Yang Hong, Haitao Wu, Weitao Wang, Xuan Zhang, Qian Chen, Yaoyu Hu, Zhengbao Zha   +6 more
wiley   +1 more source

Cytosolic DNA Sensor Upregulation Accompanies DNA Electrotransfer in B16.F10 Melanoma Cells

Molecular Therapy: Nucleic Acids, 2016
In several preclinical tumor models, antitumor effects occur after intratumoral electroporation, also known as electrotransfer, of plasmid DNA devoid of a therapeutic gene.
Katarina Znidar, Masa Bosnjak, Maja Cemazar, Loree C. Heller   +3 more
doaj   +1 more source