Results 31 to 40 of about 6,790,032 (188)
Noninvasive Gene Electrotransfer in Skin [PDF]
Human Gene Therapy Methods, 2019 The skin is considered as well suited for gene therapy and vaccination. DNA vaccines elicit both broad humoral and cellular immune responses when injected in the skin. Physical and chemical methods are needed to boost the expression. Gene electrotransfer (GET) is one of the most effective approaches.
Lise, Pasquet +5 more
openaire +2 more sources
Monopolar gene electrotransfer enhances plasmid DNA delivery to skin.
Bioelectrochemistry, 2021 A novel monopolar electroporation system and methodologies were developed for in vivo electroporation intended for potential clinical applications such as gene therapy. We hypothesized that an asymmetric anode/cathode electrode applicator geometry could produce favorable electric fields for electroporation, without the typical drawback associated with ...
A. Bulysheva +5 more
semanticscholar +4 more sources
Radiosensitizing effect of intratumoral interleukin-12 gene electrotransfer in murine sarcoma [PDF]
, 2013 BACKGROUND: Interleukin-12 (IL-12) based radiosensitization is an effective way of tumor treatment. Local cytokine production, without systemic shedding, might provide clinical benefit in radiation treatment of sarcomas.
Ales Sedlar +5 more
core +1 more source
Gene expression profiles in skeletal muscle after gene electrotransfer [PDF]
BMC Molecular Biology, 2007 Gene transfer by electroporation (DNA electrotransfer) to muscle results in high level long term transgenic expression, showing great promise for treatment of e.g. protein deficiency syndromes. However little is known about the effects of DNA electrotransfer on muscle fibres.
Hojman, Pernille +4 more
openaire +3 more sources
Gene electrotransfer: Potential for gene therapy of renal diseases [PDF]
Kidney International, 2002 The ability to pursue gene therapy has been limited by the availability of an effective and safe system for gene delivery, especially to the kidney. Electroporation is an efficient method to transfer physiologically the gene to the cells without complicated preparation.
Imai, Enyu, Isaka, Yoshitaka
openaire +2 more sources
The mERG1a channel modulates skeletal muscle MuRF1, but not MAFbx, expression. [PDF]
, 2013 INTRODUCTION: We investigated the mechanism by which the MERG1a K+ channel increases ubiquitin proteasome proteolysis (UPP). METHODS: Hindlimb suspension and electro-transfer of Merg1a cDNA into mouse gastrocnemius muscles induced atrophy.
Bradley, Kevin S +10 more
core +2 more sources
Enhancement of electroporation facilitated immunogene therapy via T-reg depletion [PDF]
, 2014 Regulatory T cells (T-regs) can negatively impact tumor antigen-specific immune responses after infiltration into tumor tissue. However, depletion of T-regs can facilitate enhanced anti-tumor responses, thus augmenting the potential for immunotherapies ...
A Testori +28 more
core +1 more source
Applied Sciences, 2022 Electroporation (EP) allows for the transport of molecules into the cytoplasm with significant effectiveness by forming transient pores in the cell membrane using electric pulses. This can be used for cellular transport (RE—reversible electroporation) or
Katarzyna Rakoczy +9 more
semanticscholar +1 more source
Effective gene immunotherapy for melanoma utilizing an advanced <i>in vivo</i> electrotransfer system. [PDF]
Mol Ther OncolHeller LC +8 more
europepmc +2 more sources
Gene Electrotransfer in 3D Reconstructed Human Dermal Tissue [PDF]
Current Gene Therapy, 2016 Gene electrotransfer into the skin is of particular interest for the development of medical applications including DNA vaccination, cancer treatment, wound healing or treatment of local skin disorders. However, such clinical applications are currently limited due to poor understanding of the mechanisms governing DNA electrotransfer within human tissue.
Madi, Moinecha +2 more
openaire +3 more sources