Results 31 to 40 of about 7,937 (203)

The mERG1a channel modulates skeletal muscle MuRF1, but not MAFbx, expression. [PDF]

, 2013
INTRODUCTION: We investigated the mechanism by which the MERG1a K+ channel increases ubiquitin proteasome proteolysis (UPP). METHODS: Hindlimb suspension and electro-transfer of Merg1a cDNA into mouse gastrocnemius muscles induced atrophy.
Bradley, Kevin S, Du, Huahua, Fujita, Naoya, Hannon, Kevin M, Hockerman, Gregory H, Jaeger, Christine, Nedele, Carrie, Pond, Amber L, Walther, Claire, Wang, Wen-Horng, Wang, Xun   +10 more
core   +2 more sources

Enhancement of electroporation facilitated immunogene therapy via T-reg depletion [PDF]

, 2014
Regulatory T cells (T-regs) can negatively impact tumor antigen-specific immune responses after infiltration into tumor tissue. However, depletion of T-regs can facilitate enhanced anti-tumor responses, thus augmenting the potential for immunotherapies ...
A Testori, AMM Eggermont, C Collins, D Alston, D M Soden, F Erdmann, F Tas, G C O' Sullivan, G Tevz, I Martínez-Lacaci, JFM Jacobs, L Finn, L Heller, L J Hall, M de Kruijf, M Sadadcharam, M Tangney, MC Whelan, MC Whelan, ML Lucas, P F Forde, R Dummer, S Bhatia, T R O' Donovan, TJ Curiel, TR Wilson, W L Byrne, W Zou, W Zou   +28 more
core   +1 more source

Gene Electrotransfer in 3D Reconstructed Human Dermal Tissue [PDF]

Current Gene Therapy, 2016
Gene electrotransfer into the skin is of particular interest for the development of medical applications including DNA vaccination, cancer treatment, wound healing or treatment of local skin disorders. However, such clinical applications are currently limited due to poor understanding of the mechanisms governing DNA electrotransfer within human tissue.
Madi, Moinecha, Rols, Marie-Pierre, Gibot, Laure   +2 more
openaire   +3 more sources

Optimization of a gene electrotransfer procedure for efficient intradermal immunization with an hTERT-based DNA vaccine in mice

Molecular Therapy: Methods & Clinical Development, 2014
DNA vaccination consists in administering an antigen-encoding plasmid in order to trigger a specific immune response. This specific vaccine strategy is of particular interest to fight against various infectious diseases and cancer.
Christophe Y Calvet, Jessie Thalmensi, Christelle Liard, Elodie Pliquet, Thomas Bestetti, Thierry Huet, Pierre Langlade-Demoyen, Lluis M Mir   +7 more
doaj   +1 more source

Differentiation of ruminant transmissible spongiform encephalopathy isolate types, including bovine spongiform encephalopathy and CH1641 scrapie [PDF]

, 2010
With increased awareness of the diversity of transmissible spongiform encephalopathy (TSE) strains in the ruminant population, comes an appreciation of the need for improved methods of differential diagnosis. Exposure to bovine spongiform encephalopathy (
Bossers, A., Jacobs, J.G., Keulen, L.J.M., van, Langeveld, J.P.M., Sauer, M., Tang, Y.   +5 more
core   +2 more sources

Electrotransfer parameters as a tool for controlled and targeted gene expression in skin

Molecular Therapy: Nucleic Acids, 2016
Skin is an attractive target for gene electrotransfer. It consists of different cell types that can be transfected, leading to various responses to gene electrotransfer. We demonstrate that these responses could be controlled by selecting the appropriate
Spela Kos, Tanja Blagus, Maja Cemazar, Ursa Lampreht Tratar, Monika Stimac, Lara Prosen, Tanja Dolinsek, Urska Kamensek, Simona Kranjc, Lars Steinstraesser, Gaëlle Vandermeulen, Véronique Préat, Gregor Sersa   +12 more
doaj   +1 more source

Fast and non-viral method to deliver plasmid DNA to human muscle-tendon graft used in anterior cruciate ligament reconstruction

European Cells & Materials, 2023
Muscle tissues attached to the proximal ends of hamstring tendon grafts are routinely discarded during anterior cruciate ligament (ACL) reconstruction. Preserving the muscle on the ACL graft was shown to improve the surgery outcomes, possibly due to the ...
A Šećerović, B Markelc, I Matić, A Frischer, A Hacobian, T Jesenko, M Cemazar, H Redl, D Marolt Presen, A Ivković   +9 more
doaj   +1 more source

A microfluidic biochip for the nanoporation of living cells [PDF]

, 2011
International audienceThis paper deals with the development of a microfluidic biochip for the exposure of living cells to nanosecond pulsed electric fields (nsPEF). When exposed to ultra short electric pulses (typical duration of 3-10ns), disturbances on
Arnaud-Cormos, Delia, Dalmay, Claire, Français, Olivier, Joubert, Vanessa, Le Pioufle, Bruno, Lévêque, Philippe, Mir, Lluis M, Silve, Aude, Villemejane, Julien   +8 more
core   +2 more sources

Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma

Mediators of Inflammation, 2017
Cancer immunotherapy is currently one of the leading approaches in cancer treatment. Gene electrotransfer of plasmids encoding interleukin 12 (IL-12) into the cells leads to the production of IL-12, which drives immune cell polarization to an antitumoral
Ursa Lampreht Tratar, Luisa Loiacono, Maja Cemazar, Urska Kamensek, Vito Michele Fazio, Gregor Sersa, Emanuela Signori   +6 more
doaj   +1 more source

Electropermeabilization of inner and outer cell membranes with microsecond pulsed electric field. Quantitative study with calcium ions [PDF]

, 2017
Microsecond pulsed electric fields (mu sPEF) permeabilize the plasma membrane (PM) and are widely used in research, medicine and biotechnology. For internal membranes permeabilization, nanosecond pulsed electric fields (nsPEF) are applied but this ...
Andrã©, Franck M., Denzi, Agnese, Hanna, Hanna, Liberti, Micaela, Mir, Lluis M.   +4 more
core   +2 more sources