Results 51 to 60 of about 6,790,032 (188)
The subnuclear localization of tRNA ligase in yeast [PDF]
, 1987 Yeast tRNA ligase is an enzyme required for tRNA splicing. A study by indirect immune fluorescence shows that this enzyme is localized in the cell nucleus. At higher resolution, studies using indirect immune electron microscopy show this nuclear location
Abelson, John, Clark, Michael W.
core +1 more source
Minicircle DNA electrotransfer for efficient tissue-targeted gene delivery [PDF]
Gene Therapy, 2012 A major issue for successful human gene therapy or genetic vaccination is a safe high-transgene expression level. Plasmid-based (non-viral) physical methods of gene transfer offered attracting approaches but their low efficiencies have limited their use in human pre-clinical trials.
Schmeer, M. +5 more
openaire +2 more sources
Single exponential decay waveform; a synergistic combination of electroporation and electrolysis (E2) for tissue ablation. [PDF]
, 2016 BackgroundElectrolytic ablation and electroporation based ablation are minimally invasive, non-thermal surgical technologies that employ electrical currents and electric fields to ablate undesirable cells in a volume of tissue.
Guenther, Enric +4 more
core +2 more sources
A comparative study of non-viral gene delivery techniques to human adipose-derived mesenchymal stem cell [PDF]
, 2014 Mesenchymal stem cells (MSCs) hold tremendous potential for therapeutic use in stem cell-based gene therapy. Ex vivo genetic modification of MSCs with beneficial genes of interest is a prerequisite for successful use of stem cell-based therapeutic ...
Abdul Halim, Nur Shuhaidatul Sarmiza +3 more
core +2 more sources
A New Method for Quantitative Immunoblotting of Endogenous α-Synuclein [PDF]
, 2013 β-Sheet-rich aggregates of α-synuclein (αSyn) are the hallmark neuropathology of Parkinson’s disease and related synucleinopathies, whereas the principal native structure of αSyn in healthy cells - unfolded monomer or α-helically folded oligomer - is ...
Dettmer, Ulf +2 more
core +5 more sources
Advanced Materials, Volume 38, Issue 6, 27 January 2026.Inspired by mussel proteins, YDC‐Gel‐Zn is a smart bone glue designed for osteoporotic implants. It adheres strongly to both metal implants and bone for initial fixation. In osteoporosis's inflammatory environment, it sequentially releases zinc ions and therapeutic peptides: the zinc modulates immune cells to reduce inflammation, while the peptides ...
Wentao Wang +9 more
wiley +1 more source
Quantification of propidium iodide delivery with millisecond electric pulses: A model study [PDF]
, 2014 A model study of propidium iodide delivery with millisecond electric pulses is presented; this work is a companion of the experimental efforts by Sadik et al. [1].
Lin, Hao, Yu, Miao
core
Beyond PD‐1/PD‐L1: New Immune Checkpoints and Therapeutic Combinations in Cancer Immunotherapy
Medicine Bulletin, Volume 2, Issue 1, Page 61-93, January 2026.ABSTRACT Recently, immune checkpoint inhibitors (ICIs), particularly PD‐1/PD‐L1 and CTLA‐4 inhibitors, have revolutionized cancer treatment, significantly improving survival rates for various malignancies. However, ICI therapies targeting single checkpoints on T cells still face numerous challenges, such as low response rates and post‐treatment ...
Yangyang Li, Zizhen Kang, Yanyun Du
wiley +1 more source
pFAR plasmids: New Eukaryotic Expression Vectors for Gene Therapy, devoid of Antibiotic Resistance Markers [PDF]
, 2008 Efficient production of eukaryotic expression vectors requires the selection of plasmid-containing bacteria. To avoid the risk of dissemination of antibiotic resistance markers, we developed a new system to produce a family of plasmids Free of Antibiotic
Corinne Marie +5 more
core +1 more source
Cancer Science, Volume 117, Issue 1, Page 63-77, January 2026.Our findings have revealed that the intracellular SNAPIN interplays with KEAP1 to promote KEAP1 protein degradation via the autophagy‐lysosomal pathway. Accordingly, NRF2 dissociates from KEAP1 for nuclear translocation, thus activating its target gene GPX4.
Xuan Liu +5 more
wiley +1 more source