Results 161 to 170 of about 620,853 (205)
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CRISPR Interference in Regulation of Bacterial Gene Expression
Molecular Biology, 2022The review is devoted to the use of the CRISPR/Cas system for obtaining knockdowns of target bacterial genes by CRISPR-mediated interference (CRISPRi). CRISPRi is based on the preservation of the ability of the inactivated dCas nuclease in complex with guide RNA to bind a target, which leads to reversible repression of the selected genes.
N I, Nadolinskaia, A V, Goncharenko
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REGULATION OF BACTERIAL GENE EXPRESSION BY RIBOSWITCHES
Annual Review of Microbiology, 2005Riboswitches are structured domains that usually reside in the noncoding regions of mRNAs, where they bind metabolites and control gene expression. Like their protein counterparts, these RNA gene control elements form highly specific binding pockets for the target metabolite and undergo allosteric changes in structure. Numerous classes of riboswitches
Ronald R. Breaker, Wade C. Winkler
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Temperature-regulated expression of bacterial virulence genes
Microbes and Infection, 2000Virulence gene expression in most bacteria is a highly regulated phenomenon, affected by a variety of parameters including osmolarity, pH, ion concentration, iron levels, growth phase, and population density. Virulence genes are also regulated by temperature, which acts as an 'on-off' switch in a manner distinct from the more general heat-shock ...
Michael E. Konkel, Kit Tilly
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Crystallization of Hfq protein: a bacterial gene-expression regulator
Acta Crystallographica Section D Biological Crystallography, 2003Hfq protein from Escherichia coli (EcoHfq) has been overproduced in E. coli, purified to homogeneity and crystallized using the hanging-drop vapour-diffusion technique. Crystallization conditions for EcoHfq were found which yielded X-ray quality crystals.
Udo Bläsi +4 more
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Regulation of bacterial gene expression by ribosome stalling and rescuing
Current Genetics, 2015Ribosome is responsible for protein synthesis and is able to monitor the sequence and structure of the nascent peptide. Such ability plays an important role in determining overall gene expression profile of the bacteria through ribosome stalling and rescuing.
Yongxin Jin +3 more
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In vivo and ex vivo regulation of bacterial virulence gene expression
Current Opinion in Microbiology, 1998Bacteria are remarkably adaptable organisms that are able to survive and multiply in diverse and sometimes hostile environments. Adaptability is determined by the complement of genetic information available to an organism and by the mechanisms that control gene expression.
Jeff F. Miller, Peggy A. Cotter
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Oxygen intervention in the regulation of gene expression: the photosynthetic bacterial paradigm
Cellular and Molecular Life Sciences (CMLS), 2004The means by which oxygen intervenes in gene expression has been examined in considerable detail in the metabolically versatile bacterium Rhodobacter sphaeroides. Three regulatory systems are now known in this organism, which are used singly and in combination to modulate genes in response to changing oxygen availability.
J. H. Zeilstra-Ryalls, S. Kaplan
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Metal Regulation of Gene Expression in Bacterial Systems
1998Metals are important in biochemical processes (da Silva and Williams, 1991). They can be cofactors of enzymatic reactions or they can be the key redox components of electron transport processes. Zinc is an example of a metal whose properties as a Lewis acid are used in the reactions of a wide variety of catalytic processes, and a quick glance through a
Blair Lawley +3 more
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Regulation of bacterial gene expression in response to oxidative stress
1994Publisher Summary This chapter presents the approaches used to study the regulation of oxidative defense genes in Escherichia coli and Salmonella typhimurium . These general approaches should be useful in examining the response to oxidative stress in other organisms and in further elucidating the response in E. coli and S. typhimurium .
Gisela Storz, Michel B. Toledano
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DNA Supercoiling as a Regulator of Bacterial Gene Expression
1987Studies of gene expression have advanced to a stage at which we can ask quite detailed questions about the factors that influence transcription and the ways in which these factors interact with each other. Such factors include not only the specific proteins that bind to regulatory sites on DNA, but also more subtle aspects of DNA structure, such as ...
Martin Gellert, Rolf Menzel
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