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Regulation of bacterial gene expression by metal–protein complexes
Molecular Microbiology, 1990SummaryMetal ions are essential cofactors in several transacting bacterial gene regulators. Upon binding of the metal, the receptor proteins act either as repressors of gene expression or, in other systems, as transcriptional activators. Other metal‐dependent regulatory proteins may function, directly or indirectly, as sensors of the cellular oxygen ...
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Bacterial expression system with tightly regulated gene expression and plasmid copy number
Gene, 2004A new Escherichia coli host/vector system has been engineered to allow tight and uniform modulation of gene expression and gamma origin (ori) plasmid copy number. Regulation of gamma ori plasmid copy number is achieved through arabinose-inducible expression of the necessary Rep protein, pi, whose gene was integrated into the chromosome of the host ...
Larry Cameron Anthony +4 more
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Regulation of Bacterial Gene Expression by Protease-Alleviated Spatial Sequestration (PASS)
ACS Synthetic Biology, 2015In natural microbial systems, conditional spatial sequestration of transcription factors enables cells to respond rapidly to changes in their environment or intracellular state by releasing presynthesized regulatory proteins. Although such a mechanism may be useful for engineering synthetic biology technologies ranging from cell-based biosensors to ...
Andrew Scarpelli +2 more
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Regulation of Bacterial Gene Expression
1989The total properties of any bacterium result from an interplay between its genome and its environment. The bacterium Escherichia coli carries approximately 3000 genes, but this total repertoire describes its potential properties and, in reality, only a small subset of this genetic information is expressed at any given moment. A primary influence of the
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Bacterial lipases from Pseudomonas: Regulation of gene expression and mechanisms of secretion
Biochimie, 2000Lipases from Pseudomonas bacteria are widely used for a variety of biotechnological applications. Overexpression in heterologous hosts like Escherichia coli failed to produce enzymatically active lipase prompting to study the molecular mechanisms underlying the regulation of lipase gene expression and secretion. The prototype lipase from P.
Karl-Erich Jaeger, Frank Rosenau
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The use of RNAs complementary to specific mRNAs to regulate the expression of individual bacterial genes [PDF]
Cell, 1984 A naturally occurring small RNA molecule ( micF RNA), complementary to the region encompassing the Shine-Dalgarno sequence and initiation codon of the ompF mRNA, is known to block the expression of that mRNA in E. coli. We have constructed a plasmid that produces a complementary RNA to the E. coli lpp mRNA (mic[Ipp] RNA). Induction of the mic(Ipp) gene
Masayori Inouye +2 more
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Bacterial Aminoacyl-tRNA Synthetases: Genes and Regulation of Expression
2014Aminoacyl-tRNA synthetases (AARS) form a class of essential enzymes whose main role is to ligate amino acids to tRNAs. This chapter reviews what is known about the AARS genes, their chromosomal localization, their organization, and the regulation of their expression.
Mathias Springer +2 more
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The role of mRNA degradation in the regulated expression of bacterial photosynthesis genes
Molecular Microbiology, 1993SummaryRegulation of gene expression in bacteria, as in eukaryotic cells, is often achieved by variation of mRNA levels. Since the steady state levels of mRNA depend on both the rate of synthesis and the rate of decay, both mechanisms are important for gene regulation. After considerable effort undertaken over many years to understand the regulation of
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Molecular Microbiology, 1990
SummaryThere has been a recent revival of interest in one of the most abundant Escherichia coli proteins, H1 (also called H‐NS). This protein was first identified many years ago as a major component of the bacterial nucleoid, and has been characterized biochemically by several groups.
Higgins, C. F. +5 more
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SummaryThere has been a recent revival of interest in one of the most abundant Escherichia coli proteins, H1 (also called H‐NS). This protein was first identified many years ago as a major component of the bacterial nucleoid, and has been characterized biochemically by several groups.
Higgins, C. F. +5 more
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Multilevel Regulation of Bacterial Gene Expression with the Combined STAR and Antisense RNA System
ACS Synthetic Biology, 2018Synthetic small RNA regulators have emerged as a versatile tool to predictably control bacterial gene expression. Owing to their simple design principles, small size, and highly orthogonal behavior, these engineered genetic parts have been incorporated into genetic circuits.
Young Je Lee +2 more
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