Results 221 to 230 of about 369,643 (266)
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CRISPR Interference in Regulation of Bacterial Gene Expression
Molecular Biology, 2022The review is devoted to the use of the CRISPR/Cas system for obtaining knockdowns of target bacterial genes by CRISPR-mediated interference (CRISPRi). CRISPRi is based on the preservation of the ability of the inactivated dCas nuclease in complex with guide RNA to bind a target, which leads to reversible repression of the selected genes.
N I, Nadolinskaia, A V, Goncharenko
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REGULATION OF BACTERIAL GENE EXPRESSION BY RIBOSWITCHES
Annual Review of Microbiology, 2005Riboswitches are structured domains that usually reside in the noncoding regions of mRNAs, where they bind metabolites and control gene expression. Like their protein counterparts, these RNA gene control elements form highly specific binding pockets for the target metabolite and undergo allosteric changes in structure. Numerous classes of riboswitches
Wade C, Winkler, Ronald R, Breaker
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Temperature-regulated expression of bacterial virulence genes
Microbes and Infection, 2000Virulence gene expression in most bacteria is a highly regulated phenomenon, affected by a variety of parameters including osmolarity, pH, ion concentration, iron levels, growth phase, and population density. Virulence genes are also regulated by temperature, which acts as an 'on-off' switch in a manner distinct from the more general heat-shock ...
M E, Konkel, K, Tilly
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Crystallization of Hfq protein: a bacterial gene-expression regulator
Acta Crystallographica Section D Biological Crystallography, 2003Hfq protein from Escherichia coli (EcoHfq) has been overproduced in E. coli, purified to homogeneity and crystallized using the hanging-drop vapour-diffusion technique. Crystallization conditions for EcoHfq were found which yielded X-ray quality crystals.
Vassilieva, Ioulia M. +4 more
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Bacterial expression system with tightly regulated gene expression and plasmid copy number
Gene, 2004A new Escherichia coli host/vector system has been engineered to allow tight and uniform modulation of gene expression and gamma origin (ori) plasmid copy number. Regulation of gamma ori plasmid copy number is achieved through arabinose-inducible expression of the necessary Rep protein, pi, whose gene was integrated into the chromosome of the host ...
Lisa M, Bowers +4 more
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Regulation of bacterial gene expression by ribosome stalling and rescuing
Current Genetics, 2015Ribosome is responsible for protein synthesis and is able to monitor the sequence and structure of the nascent peptide. Such ability plays an important role in determining overall gene expression profile of the bacteria through ribosome stalling and rescuing.
Yongxin, Jin, Shouguang, Jin, Weihui, Wu
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In vivo and ex vivo regulation of bacterial virulence gene expression
Current Opinion in Microbiology, 1998Bacteria are remarkably adaptable organisms that are able to survive and multiply in diverse and sometimes hostile environments. Adaptability is determined by the complement of genetic information available to an organism and by the mechanisms that control gene expression.
P A, Cotter, J F, Miller
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Regulation of bacterial gene expression by metal–protein complexes
Molecular Microbiology, 1990SummaryMetal ions are essential cofactors in several transacting bacterial gene regulators. Upon binding of the metal, the receptor proteins act either as repressors of gene expression or, in other systems, as transcriptional activators. Other metal‐dependent regulatory proteins may function, directly or indirectly, as sensors of the cellular oxygen ...
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Metal Regulation of Gene Expression in Bacterial Systems
1998Metals are important in biochemical processes (da Silva and Williams, 1991). They can be cofactors of enzymatic reactions or they can be the key redox components of electron transport processes. Zinc is an example of a metal whose properties as a Lewis acid are used in the reactions of a wide variety of catalytic processes, and a quick glance through a
Nigel L. Brown +3 more
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Regulation of Bacterial Gene Expression by Protease-Alleviated Spatial Sequestration (PASS)
ACS Synthetic Biology, 2015In natural microbial systems, conditional spatial sequestration of transcription factors enables cells to respond rapidly to changes in their environment or intracellular state by releasing presynthesized regulatory proteins. Although such a mechanism may be useful for engineering synthetic biology technologies ranging from cell-based biosensors to ...
Ragan A, Pitner +2 more
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