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The accessory role of the outer membrane porin protein MspD in <i>Mycobacterium smegmatis</i> zinc homeostasis. [PDF]

Microbiology (Reading)
Goethe E, Ackermann M, Bulmann L, Laarmann K, Lewin A, Luehrs J, Muesken M, Goethe R.   +7 more
europepmc   +1 more source

The IrlS2-IrlR2 two-component system is a global regulator of biofilm formation, stress adaptation, and virulence in <i>Burkholderia pseudomallei</i>. [PDF]

mSphere
Chen F, Luo J, Wang Y, Tian S, Kang X, Zhang N, Zheng W, Li W, Xia Q, Kuang D.   +9 more
europepmc   +1 more source

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CRISPR Interference in Regulation of Bacterial Gene Expression

Molecular Biology, 2022
The review is devoted to the use of the CRISPR/Cas system for obtaining knockdowns of target bacterial genes by CRISPR-mediated interference (CRISPRi). CRISPRi is based on the preservation of the ability of the inactivated dCas nuclease in complex with guide RNA to bind a target, which leads to reversible repression of the selected genes.
N I, Nadolinskaia, A V, Goncharenko
openaire   +2 more sources

REGULATION OF BACTERIAL GENE EXPRESSION BY RIBOSWITCHES

Annual Review of Microbiology, 2005
Riboswitches are structured domains that usually reside in the noncoding regions of mRNAs, where they bind metabolites and control gene expression. Like their protein counterparts, these RNA gene control elements form highly specific binding pockets for the target metabolite and undergo allosteric changes in structure. Numerous classes of riboswitches
Wade C, Winkler, Ronald R, Breaker
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Temperature-regulated expression of bacterial virulence genes

Microbes and Infection, 2000
Virulence gene expression in most bacteria is a highly regulated phenomenon, affected by a variety of parameters including osmolarity, pH, ion concentration, iron levels, growth phase, and population density. Virulence genes are also regulated by temperature, which acts as an 'on-off' switch in a manner distinct from the more general heat-shock ...
M E, Konkel, K, Tilly
openaire   +2 more sources

Crystallization of Hfq protein: a bacterial gene-expression regulator

Acta Crystallographica Section D Biological Crystallography, 2003
Hfq protein from Escherichia coli (EcoHfq) has been overproduced in E. coli, purified to homogeneity and crystallized using the hanging-drop vapour-diffusion technique. Crystallization conditions for EcoHfq were found which yielded X-ray quality crystals.
Vassilieva, Ioulia M., Nikulin, Alexey D., Bläsi, Udo, Moll, Isabella, Garber, Maria B.   +4 more
openaire   +3 more sources

Bacterial expression system with tightly regulated gene expression and plasmid copy number

Gene, 2004
A new Escherichia coli host/vector system has been engineered to allow tight and uniform modulation of gene expression and gamma origin (ori) plasmid copy number. Regulation of gamma ori plasmid copy number is achieved through arabinose-inducible expression of the necessary Rep protein, pi, whose gene was integrated into the chromosome of the host ...
Lisa M, Bowers, Kathleen, Lapoint, Larry, Anthony, Anna, Pluciennik, Marcin, Filutowicz   +4 more
openaire   +2 more sources

Regulation of bacterial gene expression by ribosome stalling and rescuing

Current Genetics, 2015
Ribosome is responsible for protein synthesis and is able to monitor the sequence and structure of the nascent peptide. Such ability plays an important role in determining overall gene expression profile of the bacteria through ribosome stalling and rescuing.
Yongxin, Jin, Shouguang, Jin, Weihui, Wu
openaire   +2 more sources