Generation of gene deletions and gene replacements in Escherichia coli O157:H7 using a temperature sensitive allelic exchange system [PDF]
, 2006 In this work we describe protocols for the generation of gene deletions and gene replacements using a temperature sensitive plasmid in Escherichia coli O157:H7.
James R. Emmerson +6 more
core +1 more source
Gene Expression: Dialing Up the Frequency [PDF]
Current Biology, 2008 Cells often respond to external signals by altering their gene expression. The external signaling information is transduced and typically encoded in concentrations of relevant transcription factors. A recent study demonstrates that, by encoding this information in the frequency with which genes 'see' a transcription factor, the expression of hundreds ...
openaire +2 more sources
From mice to humans—divergent strategies for intestinal homeostasis and regeneration
FEBS Letters, EarlyView.Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa +2 more
wiley +1 more source
Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis
FEBS Letters, EarlyView.Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee +7 more
wiley +1 more source
Stria Gravidarum Is Genetic But Not Related With Collagen Gene Polymorphism [PDF]
, 2013 BACKGROUND: Striae Gravidarum (SG) is a common problem that complicates the pregnancies. We aimed to evaluate the possible etiologic factors of SG and the possible importance of collagen polymorphism on SG.
Gungor, Cakir A. N. +6 more
core
Medycyna PracyBackground The study aimed to investigate the influence of extremely low-frequency electromagnetic fields (ELF-EMF) on clear cell renal cell carcinoma (ccRCC) by assessing alterations in gene expression and the secretion of cytokines and chemokines ...
Aleksandra Cios +7 more
doaj +1 more source
Modelling stem cell differentiation related processes—A practical overview for biologists
FEBS Letters, EarlyView.Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar +4 more
wiley +1 more source
Horizontal gene transfer and evolution of antibiotic gene clusters [PDF]
Genome sequencing projects from diverse bacteria and the release of genetically engineered microorganisms make the topic of horizontal gene transfer (HGT) fashionable.
Kallifidas, Dimitrios
core
Comparative frequency of Coagulation Factor II and Coagulation Factor V Alleles among new-born and senior citizens [PDF]
, 2005 Resistance to activated protein C is one of the most common inherited disorders associated with hereditary thrombophilia. A missense mutation in the gene coding for coagulation factor V (CF V Leiden) and which renders this procoagulant factor resistant ...
Bezzina Wettinger, Stephanie +4 more
core
Low frequency of ESRRA-C11orf20 fusion gene in ovarian carcinomas.
PLoS Biology, 2014 The identification of recurrent gene fusions in common epithelial cancers--for example, TMPRSS2/ERG in prostate cancer and EML4/ALK in nonsmall cell lung carcinomas--has raised the question of whether fusion genes are pathogenetically important also in ...
Francesca Micci +5 more
doaj +1 more source