Results 101 to 110 of about 6,900,738 (371)

Targeted transcriptome analysis using synthetic long read sequencing uncovers isoform reprograming in the progression of colon cancer

Communications Biology, 2021
Silvia Liu et al. present LoopSeq, a synthetic long-read sequencing method that generates accurate long-read transcriptome data from short Illumina reads.
Silvia Liu, Indira Wu, Yan-Ping Yu, Michael Balamotis, Baoguo Ren, Tuval Ben Yehezkel, Jian-Hua Luo   +6 more
doaj   +1 more source

Modular proteins from the Drosophila sallimus (sls) gene and their expression in muscles with different extensibility [PDF]

, 2007
The passive elasticity of the sarcomere in striated muscle is determined by large modular proteins, such as titin in vertebrates. In insects, the function of titin is divided between two shorter proteins, projectin and sallimus (Sls), which are the ...
Ayme-Southgate, Bang, Belinda Bullard, Bullard, Bullard, Bullard, Bullard, Bullard, Champagne, Christoph Burkart, Clayton, De Masi, Dickinson, Djinovic-Carugo, Feng Qiu, Granzier, Hakeda, Hao, Harlow, Henkin, Kevin Leonard, Kolmerer, Kontrogianni-Konstantopoulos, Kreisköther, Kulke, Labeit, Lakey, Lakey, Leake, Lehmann, Liu, Machado, Machado, Morin, Patel, Peckham, Petra Hååg, Rosenfeld, Saide, Shapiro, Siegfried Labeit, Sigrun Brendel, Small, Southgate, Sutter, Tatsumi, Tokayasu, Trinick, Tskhovrebova, van Straaten, Vigoreaux, Vladimir Benes, Young, Zhang   +53 more
core   +1 more source

Transcript isoform sequencing reveals widespread promoter-proximal transcriptional termination in Arabidopsis

Nature Communications, 2019
RNA polymerase II (RNAPII) transcription converts the DNA sequence of a single gene into multiple transcript isoforms that may carry alternative functions.
Q. Thomas, Ryan Ard, Jinghan Liu, Bingnan Li, Jingwen Wang, V. Pelechano, S. Marquardt   +6 more
semanticscholar   +1 more source

Targeting the MDM2‐MDM4 interaction interface reveals an otherwise therapeutically active wild‐type p53 in colorectal cancer

Molecular Oncology, EarlyView.
This study investigates an alternative approach to reactivating the oncosuppressor p53 in cancer. A short peptide targeting the association of the two p53 inhibitors, MDM2 and MDM4, induces an otherwise therapeutically active p53 with unique features that promote cell death and potentially reduce toxicity towards proliferating nontumor cells.
Sonia Valentini, Giada Mele, Marika Attili, Maria Rita Assenza, Fulvio Saccoccia, Francesca Sardina, Cinzia Rinaldo, Roberto Massari, Nicola Tirelli, Alfredo Pontecorvi, Fabiola Moretti   +10 more
wiley   +1 more source

A Quick, Affordable Method for the Quantification of Gene Isoforms

The FASEB Journal, 2015
Most genes are transcribed as several variants known as isoforms. Isoforms are transcripts from the same locus with either a different transcription start site or post‐transcriptional modifications, for example alternative splicing. As a result, isoforms might be characterized by different functions and/or stability.
Catherine Sutherland, Gaia Bistulfi
openaire   +1 more source

Multiple mRNA isoforms of the transcription activator protein CREB [PDF]

, 1992
We have characterized cDNA clones representing mouse CREB (cyclic AMP responsive element binding protein) mRNA isoforms. These include CREBA and CREBa, of which the rat and human homologues have been previously identified.
Boshart, Michael, Cole, Timothy J., Ruppert, Siegfried, Schmidt, Erika, Schütz, Günther   +4 more
core  

Isoform-specific potentiation of stem and progenitor cell engraftment by AML1/RUNX1 [PDF]

, 2007
Background: AML1/RUNX1 is the most frequently mutated gene in leukaemia and is central to the normal biology of hematopoietic stem and progenitor cells. However, the role of different AML1 isoforms within these primitive compartments is unclear.
Ailles, Bae, Blyth, Brunstein, Cai, Calkhoven, Cantor, Cashman, Chickarmane, Christiansen, de Bruijn, de Guzman, de Wynter, Demaison, Dengli Hong, Elaine Dzierzak, Freyssinier, Friedman, Fujita, Growney, Guenechea, Harada, Ichikawa, Imai, Ito, Itoh, Jin, Kasugai, Keitaro Matsuo, Levanon, Ling, Masao Seto, Miyoshi, Miyoshi, Mulloy, Nakase, Nishihara, Nishimura, Niwa, North, North, O&apos, Ohkawara, Okuda, Okuda, Osato, Persons, Piacibello, Putz, Rajeev Gupta, Reif, Rodrigues, Sasaki, Schwieger, Shinobu Tsuzuki, Song, Sorrentino, Speck, Stirewalt, Sun, Sun, Swiers, Tanaka, Tariq Enver, Taussig, Tonnelle, Tsuzuki, Wang, Wechsler, Xu, Yagi, Yin, Zhang   +72 more
core   +3 more sources

Evaluation and comparison of computational tools for RNA-seq isoform quantification

BMC Genomics, 2017
Alternatively spliced transcript isoforms are commonly observed in higher eukaryotes. The expression levels of these isoforms are key for understanding normal functions in healthy tissues and the progression of disease states.
Chi Zhang, Baohong Zhang, Lih-Ling Lin, Shanrong Zhao   +3 more
semanticscholar   +1 more source

ShcD adaptor protein drives invasion of triple negative breast cancer cells by aberrant activation of EGFR signaling

Molecular Oncology, EarlyView.
We identified adaptor protein ShcD as upregulated in triple‐negative breast cancer and found its expression to be correlated with reduced patient survival and increased invasion in cell models. Using a proteomic screen, we identified novel ShcD binding partners involved in EGFR signaling pathways.
Hayley R. Lau, Hayley S. Smith, Begüm Alural, Claire E. Martin, Laura A. New, Manali Tilak, Sara L. Banerjee, Hannah N. Robeson, Nicolas Bisson, Anne‐Claude Gingras, Jasmin Lalonde, Nina Jones   +11 more
wiley   +1 more source

A model for isoform-level differential expression analysis using RNA-seq data without pre-specifying isoform structure.

PLoS ONE, 2022
MotivationNext generation sequencing (NGS) technology has been widely used in biomedical research, particularly on those genomics-related studies. One of NGS applications is the high-throughput mRNA sequencing (RNA-seq), which is usually applied to ...
Yang Liu, Junying Wang, Song Wu, Jie Yang   +3 more
doaj   +1 more source