Results 51 to 60 of about 139,185 (274)

Genetic manipulation of Schistosoma haematobium, the neglected schistosome. [PDF]

PLoS Neglected Tropical Diseases, 2011
Minimal information on the genome and proteome of Schistosoma haematobium is available, in marked contrast to the situation with the other major species of human schistosomes for which draft genome sequences have been reported.
Gabriel Rinaldi, Tunika I Okatcha, Anastas Popratiloff, Mary A Ayuk, Sutas Suttiprapa, Victoria H Mann, Yung-san Liang, Fred A Lewis, Alex Loukas, Paul J Brindley   +9 more
doaj   +1 more source

Interaction of HS1BP3 with cortactin modulates TKS5 localisation, cell secretion and cancer malignancy

Molecular Oncology, EarlyView.
Here, we demonstrate that HS1BP3 interacts with Cortactin through a proline‐rich region (PRR3.1) and show that this interaction, and HS1BP3 itself, promote cancer cell proliferation and invasion. Inhibition of this interaction leads to build‐up of TKS5 in multivesicular endosomes and altered secretion of CD63 and CD9, providing an explanation for the ...
Arja Arnesen Løchen, Kristiane Søreng, Chiara Veroni, Laura Trachsel‐Moncho, Nagham Asp, Robin Gaupset, Lars Gustav Lyckander, Helene Knævelsrud, Lars Eftang, Anne Simonsen   +9 more
wiley   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

GeneFishing to reconstruct context specific portraits of biological processes. [PDF]

, 2019
Rapid advances in genomic technologies have led to a wealth of diverse data, from which novel discoveries can be gleaned through the application of robust statistical and computational methods.
Ashuach, Tal, Bickel, Peter J, Dose, Andrea C, Huang, Haiyan, Liu, Ke, Medina, Marisa W, Theusch, Elizabeth, Zhou, Yun   +7 more
core   +1 more source

BET bromodomain protein inhibition is a therapeutic option for medulloblastoma [PDF]

, 2013
Medulloblastoma is the most common malignant brain tumor of childhood, and represents a significant clinical challenge in pediatric oncology, since overall survival currently remains under 70%.
Althof, Kristina, Beckers, Anneleen, Eggert, Angelika, El-Hindy, Nicolai, Fleischhack, Gudrun, Henssen, Anton, Heukamp, Lukas, Odersky, Andrea, Schramm, Alexander, Schulte, Johannes H, Schäfers, Simon, Speleman, Franki, Sure, Ulrich, Thor, Theresa   +13 more
core   +4 more sources

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair. [PDF]

, 2020
Repair of double strand DNA breaks (DSBs) can result in gene disruption or gene modification via homology directed repair (HDR) from donor DNA. Altering cellular responses to DSBs may rebalance editing outcomes towards HDR and away from other repair ...
A Ferreira, A Montagnoli, A Orthwein, AC Komor, B Roberts, B Verdoodt, C Anders, C Yu, CD Richardson, CD Richardson, D Szklarczyk, DW Huang, E Lingeman, ES Koltun, FK Zimmermann, H Ghezraoui, J Song, JA Doudna, JE Haber, JP Duxin, K Jayathilaka, K Nakanishi, K Okita, KJ Livak, L Lei, L Pessoa-Brandão, LA Gilbert, LN Brandão, M Cavazzana, M Lei, M Yamada, MA DeWitt, MD Canny, MD Rainey, MH Porteus, N Hustedt, N Zielke, NK Sasi, NR Pannunzio, R Ceccaldi, RC Alver, S Hughes, S Lin, SB Thyme, T Maruyama, T Takenaka, T Tsuji, TA Day, TL Roth, TS Nambiar, U Eichenlaub-Ritter, VT Chu, W Li, X Liang, Z Romero   +54 more
core   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Cerebroventricular microinjection (CVMI) into adult zebrafish brain is an efficient misexpression method for forebrain ventricular cells.

PLoS ONE, 2011
The teleost fish Danio rerio (zebrafish) has a remarkable ability to generate newborn neurons in its brain at adult stages of its lifespan-a process called adult neurogenesis.
Caghan Kizil, Michael Brand
doaj   +1 more source

Virus-Mediated Transient Expression Techniques Enable Functional Genomics Studies and Modulations of Betalain Biosynthesis and Plant Height in Quinoa

Frontiers in Plant Science, 2021
Quinoa (Chenopodium quinoa), native to the Andean region of South America, has been recognized as a potentially important crop in terms of global food and nutrition security since it can thrive in harsh environments and has an excellent nutritional ...
Takuya Ogata, Masami Toyoshima, Chihiro Yamamizo-Oda, Yasufumi Kobayashi, Kenichiro Fujii, Kojiro Tanaka, Tsutomu Tanaka, Hiroharu Mizukoshi, Yasuo Yasui, Yukari Nagatoshi, Nobuyuki Yoshikawa, Yasunari Fujita, Yasunari Fujita   +12 more
doaj   +1 more source

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source