Results 61 to 70 of about 139,185 (274)

Standardizing CRISPR-Cas13 knockdown technique to investigate the role of cdh2 gene in pituitary development through growth hormone expression and transcription factors

Frontiers in Endocrinology
IntroductionCongenital hypopituitarism (CH) is characterized by the deficiency of pituitary hormones. Among CH patients, 85% lack a molecular diagnosis. Whole Exome Sequencing (WES) identified a homozygous variant (c.865G>A, p.Val289Ile) in the CDH2 gene, responsible for N-Cadherin production, crucial for cell-cell adhesion.
Bianca Helena Ventura Fernandes, Bianca Helena Ventura Fernandes, Bianca Helena Ventura Fernandes, Mara S. Junqueira, Calum MacRae, Luciani R. Silveira de Carvalho, Luciani R. Silveira de Carvalho   +6 more
openaire   +3 more sources

Viral Vector-based Improvement of Optic Nerve Regeneration: Characterization of Individual Axons\u27 Growth Patterns and Synaptogenesis in a Visual Target [PDF]

, 2015
Lack of axon growth ability in the central nervous system poses a major barrier to achieving functional connectivity after injury. Thus, a non-transgenic regenerative approach to reinnervating targets has important implications in clinical and research ...
Blackmore, Murray G., Luo, Xueting, Salgueiro, Yadira, Yungher, Benjamin J.   +3 more
core   +1 more source

Small molecule induced reactivation of mutant p53 in cancer cells [PDF]

, 2013
The p53 cancer mutant Y220C is an excellent paradigm for rescuing the function of conformationally unstable p53 mutants because it has a unique surface crevice that can be targeted by small-molecule stabilizers.
Adams, Alan R. Fersht, Andreas C. Joerger, Boeckler, Brown, Bullock, Bullock, Chipuk, Chuckowree, Cook, Dearth, Di Como, Emsley, Endo, Foster, Gannon, Green, Hengartner, Irina S. Chuckowree, Jahangir Amin, Joerger, Joerger, Joerger, Joerger, John Spencer, Johnson, Kaar, Kabsch, Kojima, Kojima, Lambert, Lane, Legros, Leslie, Liu, Newhouse, Oda, Petitjean, Rainer Wilcken, Rippin, Speidel, Spencer, Spencer, Spencer, Spencer, Spencer, Taylor, Van Maerken, Vassilev, Villunger, Vousden, Walerych, Wang, Wattel, Wilcken, Wilcken, Wolter, Xiangrui Liu, Yu   +58 more
core   +2 more sources

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Expression of Keratin-1 Predicts Recurrence and Treatment Response in Advanced Laryngeal Cancer: A Potential Therapeutic Target

Current Oncology
The survival rate of patients with advanced laryngeal cancer has not substantially improved over time. RNA sequencing analysis identified Keratin-1 (KRT1) as a gene potentially associated with cancer recurrence.
Eun Kyung Jung, S M Abdus Salam, Hye-Bin Jang, Joo Yeon Koo, Eshrat Jahan, Sun-Ae Kim, Ji Young Lee, Kyung-Hwa Lee, Tae Mi Yoon   +8 more
doaj   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Effect of Small Nuclear RNA 64 (SNORA64) on Apoptosis Regulator Genes in Pancreatic Cancer in Vitro [version 2; peer review: 2 approved, 1 approved with reservations]

F1000Research
Background Pancreatic cancer has a poor prognosis and is highly aggressive and deadly. Most pancreatic cancer diagnoses are adenocarcinomas, which account for more than 90 % of all cases.
Rana Alfardan
doaj   +1 more source

RNAi-mediated gene silencing in Rhynchophorus ferrugineus (Oliver) (Coleoptera: Curculionidae)

Open Life Sciences, 2017
RNA interference (RNAi) is a powerful strategy for gene function analysis, and it is also widely studied in view of a promising use in pest control. The red palm weevil, Rhynchophorus ferrugineus (Oliver) (Coleoptera: Curculionidae), is one of the most ...
Laudani Francesca, Strano Cinzia P., Edwards Martin G., Malacrinò Antonino, Campolo Orlando, Abd El Halim Hesham M., Gatehouse Angharad M. R., Palmeri Vincenzo   +7 more
doaj   +1 more source

Management of pest insects and plant diseases by non-transformative RNAi [PDF]

, 2019
Since the discovery of RNA interference (RNAi), scientists have made significant progress towards the development of this unique technology for crop protection.
Avila dos Santos, Ericmar, Cagliari, Deise, Dias, Nayma, Manzano Galdeano, Diogo, Smagghe, Guy, Zotti, Moises   +5 more
core   +2 more sources

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source