Results 211 to 220 of about 1,350,251 (258)
Some of the next articles are maybe not open access.
Plant Genomic Sequencing Using Gene‐Enriched Libraries
ChemInform, 2007AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
openaire +2 more sources
Construction of Human Naïve Antibody Gene Libraries
2018Size and variability often represent an obstacle in generating an effective antibody gene library for the detection of an abundant repertoire of antigens. Therefore, optimizing the construction of a large library is essential for the selection of high-affinity reactive fragments. Here, we report a highly efficient method for the construction of a human
Michela, Pasello +5 more
openaire +2 more sources
cDNA Libraries for Virus-Induced Gene Silencing
2010Virus-induced gene silencing (VIGS) exploits endogenous plant antiviral defense mechanisms to posttranscriptionally silence the expression of targeted plant genes. VIGS is quick and relatively easy to perform and therefore serves as a powerful tool for high-throughput functional genomics in plants.
Andrea T, Todd +2 more
openaire +2 more sources
Gene Cloning and Recombinant DNA Libraries
2023Gene cloning involves the replication of DNA fragments in host organisms to produce identical copies. It serves as a vital tool for the production of proteins, vaccines, and therapeutic treatments. On the other hand, rDNA libraries—systematic collections of cloned DNA sequences—function as extensive genomic and cDNA archives.
openaire +1 more source
Epitope Mapping with Gene Fragment Libraries
2001Linear epitopes, recognized by a large number of antibodies, are easily identified by expression of small fragments of the known antigen at the tip of filamentous phage. Immobilized antibodies, incubated with such a library of peptide-displaying phage, bind selectively to the fragments containing the epitope, therefore immobilizing the peptide-phage ...
Christoph H. Winter +1 more
openaire +1 more source
Molekuliarnaia biologiia, 1990
Main approaches and methods used for constructing "jamping" and cDNA libraries, including novel ones, that omit the employment of methylases and linkers, are presented. The advantages and drawbacks of the well-known and new lambda vectors, suitable for the purposes mentioned, are discussed.
E R, Zabarovskiĭ, R L, Allikmets
openaire +1 more source
Main approaches and methods used for constructing "jamping" and cDNA libraries, including novel ones, that omit the employment of methylases and linkers, are presented. The advantages and drawbacks of the well-known and new lambda vectors, suitable for the purposes mentioned, are discussed.
E R, Zabarovskiĭ, R L, Allikmets
openaire +1 more source
Cloning Genes From an Inner Ear cDNA Library
Archives of Otolaryngology - Head and Neck Surgery, 1993A rat inner ear complementary DNA (cDNA) library containing 1.9 x 10(6) recombinants was constructed and evaluated. Inserts averaged 2.0 (+/- 2.1) kilobases in length. A subset of inserts was screened for site of expression. Two cDNA transcripts were isolated based on cochlear expression restricted to the spiral ganglion.
A. F. Ryan +5 more
openaire +2 more sources
Gene Libraries: Coverage, Efficiency and Diversity
2006Gene libraries are a biological mechanism for generating combinatorial diversity in the immune system. However, they also bias the antibody creation process, so that they can be viewed as a way of guiding lifetime learning mechanisms. In this paper we examine the implications of this view, by examining coverage, avoidance of self, clustering and ...
Steve Cayzer, Jim Smith
openaire +1 more source
Construction of gene libraries for each human chromosome
Cytometry, 1990AbstractWe describe the construction of two complete sets of small insert, complete digest DNA libraries for each of the 24 human chromosomal types by the National Laboratory Gene Library Project. Flow sorting was used to purify the chromosomes which provided the DNA for cloning.
M A, Van Dilla, L L, Deaven
openaire +2 more sources
Selection of Recombinant Antibodies From Antibody Gene Libraries
2007After the sequencing of the human genome is completed, the research focus shifts toward the analysis of gene products. The human genome encodes more than 30,000 genes. Owing to alternative mRNA splicing and posttranslational modifications, for example, glycosylation, phoshorylation, and so on, the number of different proteins of human proteome is ...
Michael, Hust +2 more
openaire +2 more sources