The COVID-19 Gene and Drug Set Library [PDF]
SSRN Electronic Journal, 2020 Abstract The coronavirus (CoV) severe acute respiratory syndrome (SARS)-CoV-2 (COVID-19) pandemic has received rapid response by the research community to offer suggestions for repurposing of approved drugs as well as to improve our understanding of the COVID-19 viral life cycle molecular mechanisms.
Maxim V. Kuleshov +9 more
openaire +3 more sources
Role of the Schizosaccharomyces pombe F-box DNA helicase in processing recombination intermediates. [PDF]
, 2005 In an effort to identify novel genes involved in recombination repair, we isolated fission yeast Schizosaccharomyces pombe mutants sensitive to methyl methanesulfonate (MMS) and a synthetic lethal with rad2.
Carr, Antony M +6 more
core +2 more sources
Frontiers in Microbiology, 2012 Sphagnum mosses represent a main component in ombrotrophic wetlands. They harbor a specific and diverse microbial community with essential functions for the host. To understand extend and degree of host specificity, Sphagnum fallax and S.
Anastasia eBragina +6 more
doaj +1 more source
Transposase-assisted tagmentation of RNA/DNA hybrid duplexes
eLife, 2020 Tn5-mediated transposition of double-strand DNA has been widely utilized in various high-throughput sequencing applications. Here, we report that the Tn5 transposase is also capable of direct tagmentation of RNA/DNA hybrids in vitro.
Bo Lu +6 more
doaj +1 more source
An enhanced CRISPR repressor for targeted mammalian gene regulation. [PDF]
, 2018 The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor, but inefficiencies in target-gene silencing have limited its utility.
Cecchi, Ryan J +20 more
core +1 more source
On the utility of RNA sample pooling to optimize cost and statistical power in RNA sequencing experiments [PDF]
, 2020 Background: In gene expression studies, RNA sample pooling is sometimes considered because of budget constraints or lack of sufficient input material. Using microarray technology, RNA sample pooling strategies have been reported to optimize both the cost
Assefa, Alemu Takele +2 more
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PLoS ONE, 2017 Bovine viral diarrhea-mucosal disease (BVD-MD) is caused by bovine viral diarrhea virus (BVDV), and results in abortion, stillbirth, and fetal malformation in cows. Here, we constructed the phage display vector pCANTAB 5E-VHH and then transformed it into
Tiansen Li +8 more
doaj +1 more source
An improved shotgun antisense method for mutagenesis and gene identification
BioTechniques, 2020 Shotgun expression of antisense cDNA, where each transformed cell expresses a different antisense cDNA, has been used for mutagenesis and gene identification in Dictyostelium discoideum. However, the method has two limitations.
Yu Tang, Richard H Gomer
doaj +1 more source
Gene expression profile in white alpaca (Vicugna pacos) skin
Animal, 2011 A cDNA library from white alpaca (Vicugna pacos) skin was constructed using SMART technology to investigate the global gene expression profile in alpaca skin and identify genes associated with physiology of alpaca skin and pigmentation.
R. Fan +12 more
doaj +1 more source
Cloning and characterisation of two subtilisin-like protease genes from Neotyphodium lolii : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Molecular Genetics at Massey University [PDF]
, 2000 PCR amplification of Neotyphodium lolii genomic DNA with degenerate primers detected two different sequences with homology to subtilisin-like proteases. These two PCR products were used to screen a N. lolii Lp19 genomic library.
McGill, Michelle Kay
core