BMC Genomics, 2011 Background The Ahringer C. elegans RNAi feeding library prepared by cloning genomic DNA fragments has been widely used in genome-wide analysis of gene function.
Lu Yiming +9 more
doaj +1 more source
A Synthetic Gene Library Yields a Previously Unknown Glycoside Phosphorylase That Degrades and Assembles Poly-β-1,3-GlcNAc, Completing the Suite of β-Linked GlcNAc Polysaccharides. [PDF]
ACS Cent Sci, 2022 Macdonald SS +9 more
europepmc +1 more source
Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs
Nature Biotechnology, 2019 A lack of tools to precisely control gene expression has limited our ability to evaluate relationships between expression levels and phenotypes. Here, we describe an approach to titrate expression of human genes using CRISPR interference and series of ...
M. Jost +10 more
semanticscholar +1 more source
An improved method of constructing degradome library suitable for sequencing using Illumina platform
Plant Methods, 2019 Background Post-transcriptional gene regulation is one of the critical layers of overall gene expression programs and microRNAs (miRNAs) play an indispensable role in this process by guiding cleavage on the messenger RNA targets.
Yong-Fang Li +8 more
doaj +1 more source
Integration of genetically regulated gene expression and pharmacological library provides therapeutic drug candidates [PDF]
, 2021 Takahiro Konuma +2 more
openalex +1 more source
Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors
Nature Methods, 2017 Targeted and inducible regulation of mammalian gene expression is a broadly important capability. We engineered drug-inducible catalytically inactive Cpf1 nuclease fused to transcriptional activation domains to tune the expression of endogenous genes in ...
Y. Tak +9 more
semanticscholar +1 more source
Stem Cell Reports, 2017 Summary: Sickle cell anemia affects millions of people worldwide and is an emerging global health burden. As part of a large NIH-funded NextGen Consortium, we generated a diverse, comprehensive, and fully characterized library of sickle-cell-disease ...
Seonmi Park +18 more
doaj +1 more source
The Vertebrate Codex Gene Breaking Protein Trap Library For Genomic Discovery and Disease Modeling Applications [PDF]
, 2019 Noriko Ichino +26 more
openalex +1 more source
Expression of biotinylated proteins in mycobacterial gene libraries [PDF]
, 1987 Malcolm Collins
openalex +1 more source
BMC Genomics, 2008 Background Genomics of rye (Secale cereale L.) is impeded by its large nuclear genome (1C~7,900 Mbp) with prevalence of DNA repeats (> 90%). An attractive possibility is to dissect the genome to small parts after flow sorting particular chromosomes and ...
Číhalíková Jarmila +10 more
doaj +1 more source