Results 61 to 70 of about 1,268,532 (356)
Massive integration of large gene libraries in the chromosome of Escherichia coli
Microbial BiotechnologyLarge gene libraries are frequently created in Escherichia coli plasmids, which can induce cell toxicity and expression instability due to the high gene dosage.
Lidia Cerdán +2 more
doaj +1 more source
Modifications at the C-Terminus To Improve Pyrrole−Imidazole Polyamide Activity in Cell Culture [PDF]
, 2009 Pyrrole−imidazole (Py-Im) hairpin polyamides are a class of small molecule DNA minor groove binding compounds that have been shown to modulate endogenous gene expression in cell culture.
Dervan, Peter B., Jacobs, Claire S.
core +2 more sources
Molecular Oncology, EarlyView.Urine is a rich source of biomarkers for cancer detection. Tumor‐derived material is released into the bloodstream and transported to the urine. Urine can easily be collected from individuals, allowing non‐invasive cancer detection. This review discusses the rationale behind urine‐based cancer detection and its potential for cancer diagnostics ...
Birgit M. M. Wever +1 more
wiley +1 more source
Gene expression profiling en association with prion-related lesions in the medulla oblongata of symptomatic natural scrapie animals. [PDF]
, 2011 The pathogenesis of natural scrapie and other prion diseases remains unclear. Examining transcriptome variations in infected versus control animals may highlight new genes potentially involved in some of the molecular mechanisms of prion-induced ...
Acín, Cristina +10 more
core +12 more sources
AnimalsTranscription factors play important roles in the growth and development of various tissues in pigs, such as muscle, fat, and bone. A transcription-factor-scale activation library based on the clustered, regularly interspaced, short palindromic repeat ...
Yingjuan Liang +10 more
doaj +1 more source
The Impact of cDNA Normalization on Long-Read Sequencing of a Complex Transcriptome
Frontiers in Genetics, 2019 Normalization of cDNA is widely used to improve the coverage of rare transcripts in analysis of transcriptomes employing next-generation sequencing. Recently, long-read technology has been emerging as a powerful tool for sequencing and construction of ...
Nam V. Hoang +5 more
doaj +1 more source
Molecular Oncology, EarlyView.Circulating tumor DNA (ctDNA) offers a possibility for different applications in early and late stage breast cancer management. In early breast cancer tumor informed approaches are increasingly used for detecting molecular residual disease (MRD) and early recurrence. In advanced stage, ctDNA provides a possibility for monitoring disease progression and
Eva Valentina Klocker +14 more
wiley +1 more source
Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens
G3: Genes, Genomes, Genetics, 2017 The adaptation of CRISPR/SpCas9 technology to mammalian cell lines is transforming the study of human functional genomics. Pooled libraries of CRISPR guide RNAs (gRNAs) targeting human protein-coding genes and encoded in viral vectors have been used to ...
Traver Hart +31 more
doaj +1 more source
Scaling of a Fast Fourier Transform and a Pseudo-spectral Fluid Solver up to 196608 cores [PDF]
Journal of Parallel and Distributed Computing 113, 77-91, 2018, 2018 In this paper we present scaling results of a FFT library, FFTK, and a pseudospectral code, Tarang, on grid resolutions up to $8192^3$ grid using 65536 cores of Blue Gene/P and 196608 cores of Cray XC40 supercomputers. We observe that communication dominates computation, more so on the Cray XC40. The computation time scales as $T_\mathrm{comp} \sim p^{-
arxiv +1 more source
Molecular Oncology, EarlyView.We quantified and cultured circulating tumor cells (CTCs) of 62 patients with various cancer types and generated CTC‐derived tumoroid models from two salivary gland cancer patients. Cellular liquid biopsy‐derived information enabled molecular genetic assessment of systemic disease heterogeneity and functional testing for therapy selection in both ...
Nataša Stojanović Gužvić +31 more
wiley +1 more source