Chromosome-segment scanning for gain- or loss-of-function screening (CHASING)
iScienceSummary: Identifying beneficial or functional mutations for a specific species is a task always relies on the labor-intensive construction of a library containing thousands of single-gene knockout or interference strains.
Yan Xia +7 more
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Creating a RAW264.7 CRISPR-Cas9 Genome Wide Library
Bio-Protocol, 2017 The bacterial clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 genome editing tools are used in mammalian cells to knock-out specific genes of interest to elucidate gene function.
Brooke Napier , Denise Monack
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Integration of genetically regulated gene expression and pharmacological library provides therapeutic drug candidates [PDF]
, 2021 Takahiro Konuma +2 more
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Fly library boosts gene tool supply [PDF]
Nature, 2007 Massive collection of transgenic Drosophila spreads its wings. Take one complete genome sequence (Drosophila Release 4.3 in this instance), generate interfering RNA transgenes from its predicted protein-coding genes (88% coverage was achieved), and you have a very powerful tool.
openaire +1 more source
A specific group of genes respond to cold dehydration stress in cut Alstroemeria flowers whereas ambient dehydration stress accelerates developmental senescence expression patterns [PDF]
, 2010 Petal development and senescence entails a normally irreversible process. It starts with petal expansion and pigment production, and ends with nutrient remobilization and ultimately cell death.
Aharoni +75 more
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Chinese Journal of Lung Cancer, 2008 Background and objective It has been proven that nm23-H1 gene is an important metastatic-suppressed gene of lung cancer. In order to screen the differential expression genes related to nm23-H1, we constructed the suppression subtractive cDNA libraries of
Sujuan YE +8 more
doaj
Fosmid library construction and screening for the maize mutant gene Vestigial glume 1
Crop Journal, 2016 The maize mutant gene Vestigial glume 1 (Vg1) has been fine-mapped to a narrow region by map-based cloning and the candidate gene for Vg1 spanned 19.5 kb. Here we report Vg1 genomic fosmid library construction and screening.
Chaoxian Liu +4 more
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Characterisation of a putative dothistromin biosynthetic cluster : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Molecular Biology at Massey University, Palmerston North, New Zealand [PDF]
, 1999 The fungus Dothistroma pini is a key pathogen in New Zealand (and international) softwood plantations, most notably P. radiata. The mycotoxin dothistromin produced by this saprophytic fungus is believed to play a major role in its pathogenesis ...
Laarakkers, Seth
core
Journal of Limnology, 2007 A cDNA library was constructed from the glacier-dwelling eutardigrade Hypsibius klebelsbergi from more than 2000 individuals collected in the Austrian Central Alps. RNA, DNA and proteins were successively isolated by the Trizol®-method.
Hartmut GREVEN +3 more
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Selection of an Efficient AAV Vector for Robust CNS Transgene Expression
Molecular Therapy: Methods & Clinical Development, 2019 Adeno-associated virus (AAV) capsid libraries have generated improved transgene delivery vectors. We designed an AAV library construct, iTransduce, that combines a peptide library on the AAV9 capsid with a Cre cassette to enable sensitive detection of ...
Killian S. Hanlon +15 more
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