Results 331 to 340 of about 7,203,409 (405)
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Current Opinion in Genetics & Development, 2000
About two years ago, it was recognized that introduction of double-stranded RNA (dsRNA) had a potent effect on gene expression, in particular on mRNA stability. Since then, this process has been found to occur in many different organisms, and to bear a strong resemblance to a previously recognized process in plants, called cosuppression.
René F. Ketting, Ronald H.A. Plasterk
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About two years ago, it was recognized that introduction of double-stranded RNA (dsRNA) had a potent effect on gene expression, in particular on mRNA stability. Since then, this process has been found to occur in many different organisms, and to bear a strong resemblance to a previously recognized process in plants, called cosuppression.
René F. Ketting, Ronald H.A. Plasterk
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Transcriptional gene silencing mutants
Plant Molecular Biology, 2000Genetic approaches to identify molecular components of transcriptional gene silencing (TGS) in plants have yielded several Arabidopsis thaliana mutants and identified the first genes involved. All mutations found affect the maintenance of silencing and reactivate silent genes in trans.
Mittelsten Scheid, O., Paszkowski, Jerzy
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Nature, 2002
The discovery that a transcriptional repressor is turned on in prostate tumours as they become metastatic, leading to the silencing of many genes, suggests a new mechanism for tumour progression.
Jacqueline Banyard, Bruce R. Zetter
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The discovery that a transcriptional repressor is turned on in prostate tumours as they become metastatic, leading to the silencing of many genes, suggests a new mechanism for tumour progression.
Jacqueline Banyard, Bruce R. Zetter
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2016
Silencing of cardiac genes by RNA interference (RNAi) has developed into a powerful new method to treat cardiac diseases. Small interfering (si)RNAs are the inducers of RNAi, but cultured primary cardiomyocytes and heart are highly resistant to siRNA transfection.
Wolfgang Poller +3 more
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Silencing of cardiac genes by RNA interference (RNAi) has developed into a powerful new method to treat cardiac diseases. Small interfering (si)RNAs are the inducers of RNAi, but cultured primary cardiomyocytes and heart are highly resistant to siRNA transfection.
Wolfgang Poller +3 more
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Cellular and Molecular Life Sciences, 2003
A number of gene-silencing phenomena including co-suppression discovered in plants, quelling in fungi and RNA interference in animals have been revealed to have steps in common. All occur in the cytoplasm at a post-transcriptional level with the mRNAs of target genes degraded in a sequence-specific manner. Small non-coding RNA molecules demonstrated to
A. S. Pickford, COGONI, Carlo
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A number of gene-silencing phenomena including co-suppression discovered in plants, quelling in fungi and RNA interference in animals have been revealed to have steps in common. All occur in the cytoplasm at a post-transcriptional level with the mRNAs of target genes degraded in a sequence-specific manner. Small non-coding RNA molecules demonstrated to
A. S. Pickford, COGONI, Carlo
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2002
Gene silencing has evolved in a broad range of organisms probably as defense mechanisms against invasive nucleic acids. Two major strategies are utilized. Transcriptional gene silencing (TGS) acts to prevent RNA synthesis and posttranscriptional gene silencing (PTGS) acts to degrade existing RNA.
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Gene silencing has evolved in a broad range of organisms probably as defense mechanisms against invasive nucleic acids. Two major strategies are utilized. Transcriptional gene silencing (TGS) acts to prevent RNA synthesis and posttranscriptional gene silencing (PTGS) acts to degrade existing RNA.
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1995
“After such a survey one could readily come to the conclusion that the basis of the phenomenon is much too complex for profitable continuation of its investigation at this time”. [W.K. Baker (1968), in a review entitled “Position-effect variegation.”]
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“After such a survey one could readily come to the conclusion that the basis of the phenomenon is much too complex for profitable continuation of its investigation at this time”. [W.K. Baker (1968), in a review entitled “Position-effect variegation.”]
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Science, 2001
In diverse organisms, small RNAs derived from cleavage of double-stranded RNA can trigger epigenetic gene silencing in the cytoplasm and at the genome level. Small RNAs can guide posttranscriptional degradation of complementary messenger RNAs and, in plants, transcriptional gene silencing by methylation of homologous DNA sequences.
Jan M. Kooter +2 more
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In diverse organisms, small RNAs derived from cleavage of double-stranded RNA can trigger epigenetic gene silencing in the cytoplasm and at the genome level. Small RNAs can guide posttranscriptional degradation of complementary messenger RNAs and, in plants, transcriptional gene silencing by methylation of homologous DNA sequences.
Jan M. Kooter +2 more
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Methods for Gene Silencing With RNAi
2007This chapter describes a technique for synthesizing and transfecting double stranded RNA (dsRNA) for RNA interference in Sf-21 cell culture. Transfection with dsRNA only requires 1 h and the cells are usually recovered within 12 h. Suggestions for designing dsRNA are included in the methods.
William E. Bentley, John C. March
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