Results 41 to 50 of about 6,519,783 (149)

Bidirectional transcription directs both transcriptional gene activation and suppression in human cells. [PDF]

PLoS Genetics, 2008
Small RNAs targeted to gene promoters in human cells have been shown to modulate both transcriptional gene suppression and activation. However, the mechanism involved in transcriptional activation has remained poorly defined, and an endogenous RNA ...
Kevin V Morris, Sharon Santoso, Anne-Marie Turner, Chiara Pastori, Peter G Hawkins   +4 more
doaj   +1 more source

Gene silencing by CRISPR interference in mycobacteria

Nature Communications, 2015
Recombination-based tools for introducing targeted genomic mutations in Mycobacterium tuberculosis are not efficient due to higher rate of illegitimate recombination compared with homologous DNA exchange.
Eira Choudhary, P. Thakur, Madhu Pareek, Nisheeth Agarwal   +3 more
semanticscholar   +1 more source

Arabidopsis Small RNAs and Their Targets During Cyst Nematode Parasitism

Molecular Plant-Microbe Interactions, 2008
Plant-parasitic cyst nematodes induce the formation of specialized feeding cells in infected roots, which involves plant developmental processes that have been shown to be influenced by microRNAs (miRNAs) and other small RNAs.
Tarek Hewezi, Peter Howe, Tom R. Maier, Thomas J. Baum   +3 more
doaj   +1 more source

Development and application of a virus-induced gene silencing protocol for the study of gene function in narrow-leafed lupin

Plant Methods, 2021
Background Lupins are promising protein crops with an increasing amount of genomic and transcriptomic resources. The new resources facilitate the in silico identification of candidate genes controlling important agronomic traits.
Davide Mancinotti, Maria Cecilia Rodriguez, Karen Michiko Frick, Bjørn Dueholm, Ditte Goldschmidt Jepsen, Niels Agerbirk, Fernando Geu-Flores   +6 more
doaj   +1 more source

Induction of RNAi Core Machinery’s Gene Expression by Exogenous dsRNA and the Effects of Pre-exposure to dsRNA on the Gene Silencing Efficiency in the Pea Aphid (Acyrthosiphon pisum)

Frontiers in Physiology, 2019
The pea aphid, Acyrthosiphon pisum, is an important agricultural pest and biological model organism, and RNA interference (RNAi) is an important tool for functional genomics and for insect pest management. However, the efficiency of RNAi in pea aphids is
Chao Ye, Chao Ye, Xin An, Xin An, Yi-Di Jiang, Yi-Di Jiang, Bi-Yue Ding, Bi-Yue Ding, Feng Shang, Feng Shang, Olivier Christiaens, Clauvis Nji Tizi Taning, Guy Smagghe, Guy Smagghe, Guy Smagghe, Jinzhi Niu, Jinzhi Niu, Jin-Jun Wang, Jin-Jun Wang   +18 more
doaj   +1 more source

Genomic prediction in plants: opportunities for ensemble machine learning based approaches [version 2; peer review: 1 approved, 2 approved with reservations]

F1000Research, 2023
Background: Many studies have demonstrated the utility of machine learning (ML) methods for genomic prediction (GP) of various plant traits, but a clear rationale for choosing ML over conventionally used, often simpler parametric methods, is still ...
Aalt D.J. van Dijk, Shahid Mansoor, Muhammad Farooq, Dick de Ridder, Harm Nijveen   +4 more
doaj  

SMCHD1 has separable roles in chromatin architecture and gene silencing that could be targeted in disease

Nature Communications, 2023
The interplay between 3D chromatin architecture and gene silencing is incompletely understood. Here, we report a novel point mutation in the non-canonical SMC protein SMCHD1 that enhances its silencing capacity at endogenous developmental targets ...
Andres Tapia del Fierro, Bianca den Hamer, Natalia Benetti, Natasha Jansz, Kelan Chen, Tamara Beck, Hannah Vanyai, Alexandra D. Gurzau, Lucia Daxinger, Shifeng Xue, Thanh Thao Nguyen Ly, Iromi Wanigasuriya, Megan Iminitoff, Kelsey Breslin, Harald Oey, Yvonne D. Krom, Dinja van der Hoorn, Linde F. Bouwman, Timothy M. Johanson, Matthew E. Ritchie, Quentin A. Gouil, Bruno Reversade, Fabrice Prin, Timothy Mohun, Silvère M. van der Maarel, Edwina McGlinn, James M. Murphy, Andrew Keniry, Jessica C. de Greef, Marnie E. Blewitt   +29 more
doaj   +1 more source

Xist-mediated silencing requires additive functions of SPEN and Polycomb together with differentiation-dependent recruitment of SmcHD1

Cell Reports, 2022
Summary: X chromosome inactivation (XCI) is mediated by the non-coding RNA Xist, which directs chromatin modification and gene silencing in cis. The RNA binding protein SPEN and associated corepressors have a central role in Xist-mediated gene silencing.
Joseph S. Bowness, Tatyana B. Nesterova, Guifeng Wei, Lisa Rodermund, Mafalda Almeida, Heather Coker, Emma J. Carter, Artun Kadaster, Neil Brockdorff   +8 more
doaj  

Virus-induced gene silencing in Nicotiana benthamiana triggered by heterologous gene sequences from Viola philippica

Biologia Plantarum, 2019
Virus-induced gene silencing (VIGS) is a particularly useful tool for functional genomics. In the present study, we attempted to utilize this technology to infer the function of genes from Viola philippica using a tobacco rattle virus (TRV) construct ...
Q.X. Li, J. Wang, S. Zheng, N. Yang, K. Sun, C.Y. He   +5 more
doaj   +1 more source

Quantitative Analysis of Efficient Endogenous Gene Silencing in Nicotiana benthamiana Plants Using Tomato bushy stunt virus Vectors That Retain the Capsid Protein Gene

Molecular Plant-Microbe Interactions, 2007
Tomato bushy stunt virus (TBSV) coat protein (CP) replacement vectors have been used previously to silence transgenes (e.g., the green fluorescent protein gene) but have not been effective for silencing endogenous plant genes.
Daniela Pignatta, Pavan Kumar, Massimo Turina, Abhaya Dandekar, Bryce W. Falk   +4 more
doaj   +1 more source