Results 51 to 60 of about 452,517 (308)

Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.)

Plant Methods, 2022
Background Although the genome for the allotetraploid bioenergy crop switchgrass (Panicum virgatum) has been established, limitations in mutant resources have hampered in planta gene function studies toward crop optimization. Virus-induced gene silencing
Kira Tiedge, Janessa Destremps, Janet Solano-Sanchez, Magda Lisette Arce-Rodriguez, Philipp Zerbe   +4 more
doaj   +1 more source

Influence of viral genes on the cell-to-cell spread of RNA silencing [PDF]

The turnip crinkle virus-based vector TCV–GFPDCP had been devised previously to study cell-to-cell and long-distance spread of virus-induced RNA silencing.
Hong, Yiguo, Zhou, Yu, Ryabov, Eugene V., Zhang, Xuemei   +3 more
core   +1 more source

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

FEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil, Anna Corrà, Annalisa Scapolatiello, Chengxiang Qiu, Gianluca Amadei   +4 more
wiley   +1 more source

Dynamics of the VIGS-Mediated Chimeric Silencing of the Nicotiana benthamiana ChlH Gene and of the Tobacco Mosaic Virus Vector

Molecular Plant-Microbe Interactions, 2003
The ChlH gene, encoding for the H subunit of the magnesium chelatase enzyme, was silenced in Nicotiana bentahamiana plants by virus-induced gene silencing (VIGS), using tobacco mosaic virus (TMV) expression vector.
Jean-Baptiste Hiriart, Eva-Mari Aro, Kirsi Lehto   +2 more
doaj   +1 more source

Transgene Was Silenced in Hybrids between Transgenic Herbicide-Resistant Crops and Their Wild Relatives Utilizing Alien Chromosomes

Plants, 2022
The commercialization of transgenic herbicide-resistant (HR) crops may cause gene flow risk. If a transgene in progenies of transgenic crops and wild relatives is silencing, these progenies should be killed by the target herbicide, thus, the gene flow ...
Zicheng Shao, Lei Huang, Yuchi Zhang, Sheng Qiang, Xiaoling Song   +4 more
doaj   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

Catch Me If You Can! RNA Silencing-Based Improvement of Antiviral Plant Immunity

Viruses, 2019
Viruses are obligate parasites which cause a range of severe plant diseases that affect farm productivity around the world, resulting in immense annual losses of yield.
Fatima Yousif Gaffar, Aline Koch
doaj   +1 more source

PARK(ing) time–How park deficiency affects the biological clock in a Drosophila model of Parkinson's disease

FEBS Letters, EarlyView.
Drosophila park mutants serve as a model for Parkinson's disease. We used this strain to investigate the connection between oxidative stress and the circadian clock mechanism. We showed that increased oxidative stress affects the physiology of pacemaker cells, disrupting their daily structural plasticity. Lack of rhythmic signaling from pacemaker cells
Kamila Zientara, Kinga Skoczek, Elzbieta Pyza, Milena Damulewicz   +3 more
wiley   +1 more source

Induction of RNAi Core Machinery’s Gene Expression by Exogenous dsRNA and the Effects of Pre-exposure to dsRNA on the Gene Silencing Efficiency in the Pea Aphid (Acyrthosiphon pisum)

Frontiers in Physiology, 2019
The pea aphid, Acyrthosiphon pisum, is an important agricultural pest and biological model organism, and RNA interference (RNAi) is an important tool for functional genomics and for insect pest management. However, the efficiency of RNAi in pea aphids is
Chao Ye, Chao Ye, Xin An, Xin An, Yi-Di Jiang, Yi-Di Jiang, Bi-Yue Ding, Bi-Yue Ding, Feng Shang, Feng Shang, Olivier Christiaens, Clauvis Nji Tizi Taning, Guy Smagghe, Guy Smagghe, Guy Smagghe, Jinzhi Niu, Jinzhi Niu, Jin-Jun Wang, Jin-Jun Wang   +18 more
doaj   +1 more source

Efficient cloning system for construction of gene silencing vectors in Aspergillus niger

, 2008
An approach based on Gateway recombination technology to efficiently construct silencing vectors was developed for use in the biotechnologically important fungus Aspergillus niger. The transcription activator of xylanolytic and cellulolytic genes XlnR of
Oliveira, J.M., Graaff, L.H., de, Veen, D., van der, Qin Ling   +3 more
core   +1 more source