Results 91 to 100 of about 12,260,520 (401)

Targeted gene mutations in Drosophila. [PDF]

Proceedings of the National Academy of Sciences, 1989
A cloned gene can be of interest because of its expression in a particular tissue or at a certain developmental stage, or because of homology to an interesting gene from another organism. In Drosophila its location in the genome is readily determined by in situ hybridization to the banded larval salivary gland polytene chromosomes, but it is more ...
Ballinger, Dennis G., Benzer, Seymour
openaire   +4 more sources

The Mechanism of Gene Targeting in Human Somatic Cells

PLoS Genetics, 2014
Gene targeting in human somatic cells is of importance because it can be used to either delineate the loss-of-function phenotype of a gene or correct a mutated gene back to wild-type. Both of these outcomes require a form of DNA double-strand break (DSB)
Yinan Kan, B. Ruis, Sherry Lin, E. Hendrickson   +3 more
semanticscholar   +1 more source

B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses

FEBS Letters, EarlyView.
B cells sense external mechanical forces and convert them into biochemical signals through mechanotransduction. Understanding how malignant B cells respond to physical stimuli represents a groundbreaking area of research. This review examines the key mechano‐related molecules and pathways in B lymphocytes, highlights the most relevant techniques to ...
Marta Sampietro, Marco Cellani, Cristina Scielzo   +2 more
wiley   +1 more source

Targeting kidney mesangium by nanoparticles of defined size [PDF]

, 2011
Nanoparticles are being investigated for numerous medical applications and are showing potential as an emerging class of carriers for drug delivery. Investigations on how the physicochemical properties (e.g., size, surface charge, shape, and density of ...
C. H. J. Choi, Chen, Choi, Davis, Davis, J. E. Zuckerman, Kanwar, Lahdenkari, Luft, M. E. Davis, Nurkiewicz, Ogawa, P. Webster, Peer, Ruckerl, Schipper, Schreiner, Soo Choi, Tan, Zimmermann   +19 more
core   +2 more sources

The thioredoxin‐like and one glutaredoxin domain are required to rescue the iron‐starvation phenotype of HeLa GLRX3 knock out cells

FEBS Letters, EarlyView.
Glutaredoxin (Grx) 3 proteins contain a thioredoxin domain and one to three class II Grx domains. These proteins play a crucial role in iron homeostasis in eukaryotic cells. In human Grx3, at least one of the two Grx domains, together with the thioredoxin domain, is essential for its function in iron metabolism.
Laura Magdalena Jordt, Manuela Gellert, Finja Zelms, Sander Bekeschus, Christopher Horst Lillig   +4 more
wiley   +1 more source

Kidney lipids in galactosylceramide synthase-deficient mice: absence of galactosylsulfatide and compensatory increase in more polar sulfoglycolipids1

Journal of Lipid Research, 2000
UDP-galactose:ceramide galactosyltransferase (CGT) catalyzes the final step in the synthesis of galactosylceramide (GalCer). It has previously been shown that CGT-deficient mice do not synthesize GalCer and its sulfated derivative GalCer I3-sulfate ...
Keiko Tadano-Aritomi, Toshiyuki Hikita, Hirokazu Fujimoto, Kunihiko Suzuki, Kohji Motegi, Ineo Ishizuka   +5 more
doaj  

Lack of RNA-DNA oligonucleotide (chimeraplast) mutagenic activity in mouse embryos [PDF]

, 2005
There are numerous reports of the use of RNA-DNA oligonucleoticles (chimeraplasts) to correct point mutations in vitro and in vivo, including the human apolipoprotein E gene (ApoE).
Owen, JS, Simons, JP, Tagalakis, AD
core   +1 more source

A CRISPR-Cas9 Gene Drive System Targeting Female Reproduction in the Malaria Mosquito vector Anopheles gambiae

Nature Biotechnology, 2015
Gene drive systems that enable super-Mendelian inheritance of a transgene have the potential to modify insect populations over a timeframe of a few years.
Andrew M. Hammond, R. Galizi, Kyros Kyrou, A. Simoni, C. Siniscalchi, D. Katsanos, Matthew Gribble, D. Baker, E. Marois, S. Russell, A. Burt, N. Windbichler, A. Crisanti, T. Nolan   +13 more
semanticscholar   +1 more source

Evolutionary interplay between viruses and R‐loops

FEBS Letters, EarlyView.
Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi, Zita Képes, Zoltán Szabó, Eszter Csoma, Lóránt Székvölgyi   +4 more
wiley   +1 more source

A Protein Kinase Cε/Protein Kinase D3 Signalling Axis Modulates RhoA Activity During Cytokinesis

Biomedicines
Background: Protein kinase D3 (PKD3) is a member of the PKD family that has been implicated in many intracellular signalling pathways. However, defined statements regarding PKD isoform specificity and in vivo functions are rare.
Ursula Braun, Michael Leitges
doaj   +1 more source