Results 91 to 100 of about 708,347 (229)
Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi. [PDF]
, 2015 Gene knockout strategies, RNAi and rescue experiments are all employed to study mammalian gene function. However, the disadvantages of these approaches include: loss of function adaptation, reduced viability and gene overexpression that rarely matches ...
Dowdy, Steven F +5 more
core +1 more source
Verification of DNA motifs in Arabidopsis using CRISPR/Cas9-mediated mutagenesis. [PDF]
, 2018 Transcription factors (TFs) and chromatin-modifying factors (CMFs) access chromatin by recognizing specific DNA motifs in their target genes. Chromatin immunoprecipitation followed by next-generation sequencing (ChIP-seq) has been widely used to discover
Chen, Chen +5 more
core +2 more sources
Functional Characterization of Cryptococcal Genes: Then and Now
Frontiers in Microbiology, 2018 Site-directed mutagenesis enables researchers to switch a gene of interest off for functional characterization of the gene. In the pathogenic yeasts, Cryptococcus neoformans and sister species C.
Lukas M. du Plooy +3 more
doaj +1 more source
Screening for the optimal siRNA targeting a novel gene (HA117) and construction and evaluation of a derivative recombinant adenovirus [PDF]
, 2010 We found a novel gene named as HA117 in our previous research. At this study, we screened for an optimal siRNA targeting the novel gene HA117 using the pSOS-HUS method, verified the results of pSOS-HUS siRNA screening for optimal affinity for the target ...
Gaihuan Zheng +4 more
core +1 more source
Microglia targeting by adeno-associated viral vectors
Frontiers in ImmunologyMicroglia play a crucial role in maintaining homeostasis of the central nervous system and they are actively involved in shaping the brain’s inflammatory response to stress.
Maria Stamataki +3 more
doaj +1 more source
Distinct Brca1 Mutations Differentially Reduce Hematopoietic Stem Cell Function
Cell Reports, 2017 BRCA1 is a well-known DNA repair pathway component and a tissue-specific tumor suppressor. However, its role in hematopoiesis is uncertain. Here, we report that a cohort of patients heterozygous for BRCA1 mutations experienced more hematopoietic toxicity
Victoria E. Mgbemena +5 more
doaj +1 more source
Journal of Lipid Research, 2000 UDP-galactose:ceramide galactosyltransferase (CGT) catalyzes the final step in the synthesis of galactosylceramide (GalCer). It has previously been shown that CGT-deficient mice do not synthesize GalCer and its sulfated derivative GalCer I3-sulfate ...
Keiko Tadano-Aritomi +5 more
doaj
The evolution of microRNA-regulation in duplicated genes facilitates expression divergence [PDF]
arXiv, 2008 Background: The evolution of microRNA regulation in metazoans is a mysterious process: MicroRNA sequences are highly conserved among distal organisms, but on the other hand, there is no evident conservation of their targets. Results: We study this extensive rewiring of the microRNA regulatory network by analyzing the evolutionary trajectories of ...
arxiv
A CRISPR Approach to Gene Targeting [PDF]
Molecular Therapy, 2012 It is getting easier and easier to determine complete genome sequences—of model organisms, animals and plants of commercial importance, and humans: Craig Venter, Jim Watson, the 1000 Genome Project, soon you and me. Now that researchers have all this information at hand, the focus has shifted in many cases to manipulating particular sequences to ...
openaire +3 more sources
Construction of an Insertion Vector for Gene Targeting of Chicken Lens-specific Gene
The Journal of Poultry Science, 2010 Production of gene-targeted chickens is considered to be valuable for studies in both biological science and industry, but it is yet to be achieved. In this study, an insertion vector for gene targeting of chicken lens-specific gene, delta1-crystallin ...
Yuji Fukushima +4 more
doaj +1 more source