Distinct Brca1 Mutations Differentially Reduce Hematopoietic Stem Cell Function
Cell Reports, 2017 BRCA1 is a well-known DNA repair pathway component and a tissue-specific tumor suppressor. However, its role in hematopoiesis is uncertain. Here, we report that a cohort of patients heterozygous for BRCA1 mutations experienced more hematopoietic toxicity
Victoria E. Mgbemena +5 more
doaj +1 more source
Conditional gene targeting. [PDF]
Journal of Clinical Investigation, 1996 Rajewsky, K. +6 more
openaire +3 more sources
Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis
FEBS Letters, EarlyView.Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee +7 more
wiley +1 more source
Delivery of human apolipoprotein (apo) E to liver by an [E1(-), E3(-), polymerase(-), pTP(-)] adenovirus vector containing a liver-specific promoter inhibits atherogenesis in immunocompetent apoE-deficient mice [PDF]
, 2006 Recombinant adenovirus (rAd)-mediated apoE gene transfer to the liver of apoE(-/-) mice is anti-atherogenic. However, first generation rAd vectors were associated with immune clearance of transduced hepatocytes, while an improved [E1(-), E3(-) polymerase(
Amalfitano, A +4 more
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Functional Characterization of Cryptococcal Genes: Then and Now
Frontiers in Microbiology, 2018 Site-directed mutagenesis enables researchers to switch a gene of interest off for functional characterization of the gene. In the pathogenic yeasts, Cryptococcus neoformans and sister species C.
Lukas M. du Plooy +3 more
doaj +1 more source
α‐Synuclein aggregation landscape from phase separation to neurotoxic intermediates
FEBS Letters, EarlyView.Alpha‐synuclein aggregation in Parkinson's disease involves a complex landscape of transient intermediates, including oligomers, fibrils and liquid–liquid phase separation (LLPS). A view is emerging in which LLPS maturation into solid‐like condensates may contribute to the formation of neurotoxic species.
Silvia Arino +2 more
wiley +1 more source
Conditional mutagenesis in the immune system: targeting the expression of the iCre2 recombinase to neutrophils and macrophages [PDF]
, 2010 Conditional mutagenesis allows the introduction of tissue specific mutations in the mouse and is of crucial importance in converting genome sequence information into functional data for biomedical research.
Könitzer, J.D.
core
Gene Therapy: The Potential Applicability of Gene Transfer Technology to the Human Germline
International Journal of Medical Sciences, 2004 The theoretical possibility of applying gene transfer methodologies to the human germline is explored. Transgenic methods for genetically manipulating embryos may in principle be applied to humans.
doaj
Microglia targeting by adeno-associated viral vectors
Frontiers in ImmunologyMicroglia play a crucial role in maintaining homeostasis of the central nervous system and they are actively involved in shaping the brain’s inflammatory response to stress.
Maria Stamataki +3 more
doaj +1 more source
FEBS Letters, EarlyView.Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley +1 more source