Results 121 to 130 of about 1,756,365 (358)

Lipofection improves gene targeting efficiency in E14 TG2a mouse embryonic stem cells [PDF]

, 2009
Electroporation has been the method of election for transfection of murine embryonic stem cells for over 15 years; however, it is a time consuming protocol because it requires large amounts of DNA and cells, as well as expensive and delicate equipment ...
ALONSO MORALES, ROGELIO ALEJANDRO, ALONSO MORALES, ROGELIO ALEJANDRO, BARBABOSA PLIEGO, ALBERTO, BARBABOSA PLIEGO, ALBERTO, Cajero Juárez, Marcos, Cajero Juárez, Marcos, LOPEZ HEYDECK, SANDRA MARICRUZ, LOPEZ HEYDECK, SANDRA MARICRUZ, MARTINEZ CASTAÑEDA, JOSE SIMON, MARTINEZ CASTAÑEDA, JOSE SIMON, Robles González, Jose Francisco, Robles González, Jose Francisco, VAZQUEZ CHAGOYAN, JUAN CARLOS, VAZQUEZ CHAGOYAN, JUAN CARLOS   +13 more
core  

The newfound relationship between extrachromosomal DNAs and excised signal circles

FEBS Letters, EarlyView.
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley   +1 more source

An Engineered RNase P Ribozyme Effectively Reduces Human Coronavirus 229E Gene Expression and Growth in Human Cells

Zoonotic Diseases
The human coronavirus 229E (HCoV-229E) is a member of the human coronavirus family that includes SARS-CoV-2, the causative agent of COVID-19. Developing antiviral strategies and compounds is crucial to treat and prevent HCoV-229E infections and the ...
Yujun Liu, Bin Yan, Hao Gong, Fenyong Liu   +3 more
doaj   +1 more source

Supplementary Figure S4 from A Four-gene Decision Tree Signature Classification of Triple-negative Breast Cancer: Implications for Targeted Therapeutics

, 2023
Jelmar Quist, Hasan Mirza, Maggie C.U. Cheang, Melinda L. Telli, Joyce O’Shaughnessy, Christopher J. Lord, Andrew Tutt, Anita Grigoriadis   +7 more
openalex   +1 more source

Botrytis cinerea small RNAs are associated with tomato AGO1 and silence tomato defense-related target genes supporting cross-kingdom RNAi [PDF]

, 2023
Baoye He, Qiang Cai, Arne Weiberg, Wei Li, An-Po Cheng, Shouqiang Ouyang, Katherine A. Borkovich, Jason Stajich, Cei Abreu‐Goodger, Hailing Jin   +9 more
openalex   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Relationship between target antigens and major histocompatibility complex (MHC) class II genes in producing two pathogenic antibodies simultaneously [PDF]

, 2010
Labib R. Zakka, Derin B. Keskin, Pedro A. Reche, A. R. Ahmed   +3 more
openalex   +1 more source

Supplementary Table S2 from Infant High-Grade Gliomas Comprise Multiple Subgroups Characterized by Novel Targetable Gene Fusions and Favorable Outcomes

, 2023
Matthew Clarke, Alan Mackay, Britta Ismer, Jessica C. Pickles, Ruth Tatevossian, Scott Newman, Tejus Bale, Iris Stoler, Elisa Izquierdo, Sara Temelso, Diana Carvalho, Valeria Molinari, Anna Burford, Louise Howell, Alex Virasami, Amy R. Fairchild, Aimee Avery, Jane Chalker, Mark Kristiansen, Kelly Haupfear, James Dalton, Wilda Orisme, Ji Wen, Michael Hubank, Kathreena M. Kurian, Catherine Rowe, Mellissa Maybury, Stephen Crosier, Jeffrey Knipstein, Ulrich Schüller, Uwe Kordes, David E. Kram, Matija Snuderl, Leslie Bridges, Andrew Martin, Lawrence J. Doey, Safa Al‐Sarraj, Christopher Chandler, Bassel Zebian, Claire Cairns, Rachael Natrajan, Jessica K.R. Boult, Simon P. Robinson, Martin Sill, Ira J. Dunkel, Stephen W. Gilheeney, Marc K. Rosenblum, Debbie Hughes, Paula Proszek, Tobey J. MacDonald, Matthias Preusser, Christine Haberler, Irene Slavc, Roger J. Packer, Ho‐Keung Ng, Shani Caspi, Mara Popović, Barbara Faganel Kotnik, Matthew D. Wood, Lissa Baird, Monika A. Davare, David A. Solomon, Thale Kristin Olsen, Petter Brandal, Michael Farrell, Jane Cryan, Michael Capra, Michael Karremann, Jens Schittenhelm, Martin U. Schuhmann, Martin Ebinger, Winand N.M. Dinjens, Kornelius Kerl, Simone Hettmer, Torsten Pietsch, Felipe Andreiuolo, Pablo Hernáiz Driever, Andrey Korshunov, Lotte Hiddingh, Barbara C. Worst, Dominik Sturm, Marc Zuckermann, Olaf Witt, Tabitha Bloom, Clare Mitchell, Evelina Miele, Giovanna Stefania Colafati, Francesca Diomedi‐Camassei, Simon Bailey, Andrew S. Moore, Tim Hassall, Stephen P. Lowis, Maria Tsoli, Mark J. Cowley, David S. Ziegler, Matthias A. Karajannis, Kristian Aquilina, Darren Hargrave, Fernando Carceller, Lynley V. Marshall   +99 more
openalex   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Challenges for Sensitive Quantification of Gene Editing “Off‐Target” Activity [PDF]

, 2017
Shengdar Q. Tsai
openalex   +1 more source