Results 261 to 270 of about 1,207,125 (312)
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Approaches for gene targeting and targeted gene expression in plants
GM Crops, 2011Transgenic science and technology are fundamental to state-of-the-art plant molecular genetics and crop improvement. The new generation of technology endeavors to introduce genes 'stably' into 'site-specific' locations and in 'single copy' without the integration of extraneous vector 'backbone' sequences or selectable markers and with a 'predictable ...
Amjad Masood, Husaini +3 more
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Gene Targeting in Neuroendocrinology
Comprehensive Physiology, 2015ABSTRACT Research in neuroendocrinology faces particular challenges due to the complex interactions between cells in the hypothalamus, in the pituitary gland and in peripheral tissues. Within the hypothalamus alone, attempting to target a specific neuronal cell type can be problematic due to the heterogeneous ...
Michael, Candlish +3 more
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British Medical Bulletin, 1995
Melanin biosynthesis is limited to melanocytes partly as a consequence of transcriptional regulation of the enzymes involved in this pathway. Promoter sequences of these enzyme genes may be utilised to drive expression of complementary DNA coding for therapeutic genes so as to provide transcriptional targeting. We have used the 5'-flanking sequences of
I R, Hart, R G, Vile
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Melanin biosynthesis is limited to melanocytes partly as a consequence of transcriptional regulation of the enzymes involved in this pathway. Promoter sequences of these enzyme genes may be utilised to drive expression of complementary DNA coding for therapeutic genes so as to provide transcriptional targeting. We have used the 5'-flanking sequences of
I R, Hart, R G, Vile
openaire +2 more sources
Trends in Biochemical Sciences, 1997
The myc family of proto-oncogenes belongs to the basic helix-loop-helix leucine-zipper (bHLHZ) class of transcription factors. Myc proteins function as transcriptional activators through heterodimerization with Max, but might also act as negative regulators of transcription.
C, Grandori, R N, Eisenman
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The myc family of proto-oncogenes belongs to the basic helix-loop-helix leucine-zipper (bHLHZ) class of transcription factors. Myc proteins function as transcriptional activators through heterodimerization with Max, but might also act as negative regulators of transcription.
C, Grandori, R N, Eisenman
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2003
Gene targeting in mouse ES cells is a powerful method for studying gene function in vivo. For the novice, this combination of molecular biology, specialized tissue-culture cell lines, and mouse reproductive biology can be daunting. We present a straightforward, one might say constrained, guide for novices of gene targeting to generate a null allele in ...
S S, Cheah, R R, Behringer
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Gene targeting in mouse ES cells is a powerful method for studying gene function in vivo. For the novice, this combination of molecular biology, specialized tissue-culture cell lines, and mouse reproductive biology can be daunting. We present a straightforward, one might say constrained, guide for novices of gene targeting to generate a null allele in ...
S S, Cheah, R R, Behringer
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2011
Establishment of methods to inactivate genes by homologous recombination in embryonic stem (ES) cells has provided great advantages to the field of mouse genetics. Using this technology, a number of null mutant mice, so-called knock-out mice, have been generated.
Anne E, Griep +3 more
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Establishment of methods to inactivate genes by homologous recombination in embryonic stem (ES) cells has provided great advantages to the field of mouse genetics. Using this technology, a number of null mutant mice, so-called knock-out mice, have been generated.
Anne E, Griep +3 more
openaire +2 more sources
Targeted gene regulation and gene ablation
Trends in Endocrinology & Metabolism, 2001Investigations of the mechanisms involved in appropriate, developmentally regulated tissue-specific gene transcription have laid the foundations for transgenic and gene-therapy technologies directing specific induction or ablation of genes of interest in a tissue-restricted manner.
F J, DeMayo, S Y, Tsai
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Altering Genes in Animals by Gene Targeting
Annual Review of Immunology, 1992It is possible to create mice in which a planned alteration has been introduced at a specific locus in the genome. The planned alteration is first introduced into the genome of mouse embryonic stem (ES) cells in tissue culture. This is accomplished by homologous recombination between the targeted genomic locus and DNA introduced into the ES cells ...
B H, Koller, O, Smithies
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Gene targeting in Arabidopsis thaliana
Molecular and General Genetics MGG, 1992Gene targeting of a chromosomally integrated transgene in Arabidopsis thaliana is reported. A chimeric gene consisting of the promoter of the 35S RNA of CaMV, the polyadenylation signal of the octopine synthase gene and the coding region of the bacterial hygromycin phosphotransferase gene (hpt), which was rendered non-functional by deletion of 19 bp ...
Halfter, U., Morris, P., Willmitzer, L.
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