Results 261 to 270 of about 1,734,183 (310)
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British Medical Bulletin, 1995
Melanin biosynthesis is limited to melanocytes partly as a consequence of transcriptional regulation of the enzymes involved in this pathway. Promoter sequences of these enzyme genes may be utilised to drive expression of complementary DNA coding for therapeutic genes so as to provide transcriptional targeting. We have used the 5'-flanking sequences of
I R, Hart, R G, Vile
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Melanin biosynthesis is limited to melanocytes partly as a consequence of transcriptional regulation of the enzymes involved in this pathway. Promoter sequences of these enzyme genes may be utilised to drive expression of complementary DNA coding for therapeutic genes so as to provide transcriptional targeting. We have used the 5'-flanking sequences of
I R, Hart, R G, Vile
openaire +2 more sources
Trends in Biochemical Sciences, 1997
The myc family of proto-oncogenes belongs to the basic helix-loop-helix leucine-zipper (bHLHZ) class of transcription factors. Myc proteins function as transcriptional activators through heterodimerization with Max, but might also act as negative regulators of transcription.
C, Grandori, R N, Eisenman
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The myc family of proto-oncogenes belongs to the basic helix-loop-helix leucine-zipper (bHLHZ) class of transcription factors. Myc proteins function as transcriptional activators through heterodimerization with Max, but might also act as negative regulators of transcription.
C, Grandori, R N, Eisenman
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2003
Gene targeting in mouse ES cells is a powerful method for studying gene function in vivo. For the novice, this combination of molecular biology, specialized tissue-culture cell lines, and mouse reproductive biology can be daunting. We present a straightforward, one might say constrained, guide for novices of gene targeting to generate a null allele in ...
S S, Cheah, R R, Behringer
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Gene targeting in mouse ES cells is a powerful method for studying gene function in vivo. For the novice, this combination of molecular biology, specialized tissue-culture cell lines, and mouse reproductive biology can be daunting. We present a straightforward, one might say constrained, guide for novices of gene targeting to generate a null allele in ...
S S, Cheah, R R, Behringer
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2011
Establishment of methods to inactivate genes by homologous recombination in embryonic stem (ES) cells has provided great advantages to the field of mouse genetics. Using this technology, a number of null mutant mice, so-called knock-out mice, have been generated.
Anne E, Griep +3 more
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Establishment of methods to inactivate genes by homologous recombination in embryonic stem (ES) cells has provided great advantages to the field of mouse genetics. Using this technology, a number of null mutant mice, so-called knock-out mice, have been generated.
Anne E, Griep +3 more
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Targeted gene regulation and gene ablation
Trends in Endocrinology & Metabolism, 2001Investigations of the mechanisms involved in appropriate, developmentally regulated tissue-specific gene transcription have laid the foundations for transgenic and gene-therapy technologies directing specific induction or ablation of genes of interest in a tissue-restricted manner.
F J, DeMayo, S Y, Tsai
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Altering Genes in Animals by Gene Targeting
Annual Review of Immunology, 1992It is possible to create mice in which a planned alteration has been introduced at a specific locus in the genome. The planned alteration is first introduced into the genome of mouse embryonic stem (ES) cells in tissue culture. This is accomplished by homologous recombination between the targeted genomic locus and DNA introduced into the ES cells ...
B H, Koller, O, Smithies
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2009
Gene targeting in mice has been used extensively to elucidate gene function in vivo. However, for gene targeting to be successful, the targeting vector must be carefully designed. This chapter addresses the rationale behind designing targeting vectors, detailing the essential components, and highlighting specific considerations for different types of ...
Simon, J. +2 more
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Gene targeting in mice has been used extensively to elucidate gene function in vivo. However, for gene targeting to be successful, the targeting vector must be carefully designed. This chapter addresses the rationale behind designing targeting vectors, detailing the essential components, and highlighting specific considerations for different types of ...
Simon, J. +2 more
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Gene targeting in Arabidopsis thaliana
Molecular and General Genetics MGG, 1992Gene targeting of a chromosomally integrated transgene in Arabidopsis thaliana is reported. A chimeric gene consisting of the promoter of the 35S RNA of CaMV, the polyadenylation signal of the octopine synthase gene and the coding region of the bacterial hygromycin phosphotransferase gene (hpt), which was rendered non-functional by deletion of 19 bp ...
Halfter, U., Morris, P., Willmitzer, L.
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Advances in Targeted Gene Delivery
Current Drug Delivery, 2019Gene therapy has the potential to treat both acquired and inherited genetic diseases. Generally, two types of gene delivery vectors are used - viral vectors and non-viral vectors. Non-viral gene delivery systems have attracted significant interest (e.g.
Ara Begum, Anjuman +3 more
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