Results 31 to 40 of about 12,501,640 (402)

CRISPR-Cas9-mediated functional dissection of 3'-UTRs. [PDF]

, 2017
Many studies using reporter assays have demonstrated that 3' untranslated regions (3'-UTRs) regulate gene expression by controlling mRNA stability and translation.
Ahituv, Nadav, Biton, Anne, Erle, David J, Siegel, David, Tonqueze, Olivier Le, Zaitlen, Noah, Zhao, Wenxue   +6 more
core   +1 more source

Efficient TALEN-mediated gene targeting of chicken primordial germ cells

Development, 2017
In this work we use TALE nucleases (TALENs) to target a reporter construct to the DDX4 (vasa) locus in chicken primordial germ cells (PGCs). Vasa is a key germ cell determinant in many animal species and is posited to control avian germ cell formation ...
L. Taylor, D. F. Carlson, S. Nandi, A. Sherman, S. Fahrenkrug, M. McGrew   +5 more
semanticscholar   +1 more source

Targeting casein kinase 1 for cancer therapy: current strategies and future perspectives

Frontiers in Oncology, 2023
Casein Kinase 1 (CK1) is a family of serine/threonine protein kinases that play a crucial role in various cellular processes, including cell proliferation, survival, and metabolism.
Ngo Hoang Long, Sook-Jeong Lee
doaj   +1 more source

Chimeric nucleases stimulate gene targeting in human cells [PDF]

, 2002
Gene targeting is a powerful technique to introduce genetic change into the genome of eukaryotic cells. It is widely used to create defined mutations in murine embryonic stem cells and theoretically could be used to create or repair mutations in ...
Baltimore, David, Porteus, Matthew
core   +2 more sources

Molecular bases of low production rates of apolipoprotein B-100 and truncated apoB-82 in a mutant HepG2 cell line generated by targeted modification of the apolipoprotein B gene

Journal of Lipid Research, 1999
In subjects with familial hypobetalipoproteinemia heterozygous for truncated forms of apolipoprotein B, both apoB-100 and the truncated forms are produced at lower than expected rates.
Rai Ajit K. Srivastava, Neelam Srivastava, Maurizio Averna, Angelo B. Cefalu, Gustav Schonfeld   +4 more
doaj   +1 more source

Gene targeting, genome editing: from Dolly to editors

Transgenic research, 2016
One of the most powerful strategies to investigate biology we have as scientists, is the ability to transfer genetic material in a controlled and deliberate manner between organisms.
W. Tan, C. Proudfoot, S. Lillico, B. Whitelaw   +3 more
semanticscholar   +1 more source

Primer selection impacts specific population abundances but not community dynamics in a monthly time-series 16S rRNA gene amplicon analysis of coastal marine bacterioplankton. [PDF]

, 2018
Primers targeting the 16S small subunit ribosomal RNA marker gene, used to characterize bacterial and archaeal communities, have recently been re-evaluated for marine planktonic habitats.
Carlson, Craig A, Nelson, Craig E, Wear, Emma K, Wilbanks, Elizabeth G   +3 more
core   +1 more source

Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells

Development, 2017
Mammalian neural stem cell (NSC) lines provide a tractable model for discovery across stem cell and developmental biology, regenerative medicine and neuroscience.
R. Bressan, P. Dewari, M. Kalantzaki, E. Gangoso, Mantas Matjusaitis, Claudia Garcia-Diaz, Carla Blin, Vivien Grant, H. Bulstrode, Sabine Gogolok, W. Skarnes, S. Pollard   +11 more
semanticscholar   +1 more source

Kidney lipids in galactosylceramide synthase-deficient mice: absence of galactosylsulfatide and compensatory increase in more polar sulfoglycolipids1

Journal of Lipid Research, 2000
UDP-galactose:ceramide galactosyltransferase (CGT) catalyzes the final step in the synthesis of galactosylceramide (GalCer). It has previously been shown that CGT-deficient mice do not synthesize GalCer and its sulfated derivative GalCer I3-sulfate ...
Keiko Tadano-Aritomi, Toshiyuki Hikita, Hirokazu Fujimoto, Kunihiko Suzuki, Kohji Motegi, Ineo Ishizuka   +5 more
doaj   +1 more source

Enhancing HR Frequency for Precise Genome Editing in Plants

Frontiers in Plant Science, 2022
Gene-editing tools, such as Zinc-fingers, TALENs, and CRISPR-Cas, have fostered a new frontier in the genetic improvement of plants across the tree of life.
Hao Chen, Hao Chen, Matthew Neubauer, Jack P. Wang, Jack P. Wang   +4 more
doaj   +1 more source