Results 41 to 50 of about 708,347 (229)
G3: Genes, Genomes, Genetics, 2019 Precise allele replacement (genome editing), without unwanted changes to the genome, provides a powerful tool to define the functions of DNA elements and encoded factors in their normal biological context.
Aaron J. Storey +4 more
doaj +1 more source
Stochastic model of transcription factor-regulated gene expression [PDF]
, 2006 We consider a stochastic model of transcription factor (TF)-regulated gene expression. The model describes two genes: Gene A and Gene B which synthesize the TFs and the target gene proteins respectively. We show through analytic calculations that the TF fluctuations have a significant effect on the distribution of the target gene protein levels when ...
arxiv +1 more source
Chimeric nucleases stimulate gene targeting in human cells [PDF]
, 2002 Gene targeting is a powerful technique to introduce genetic change into the genome of eukaryotic cells. It is widely used to create defined mutations in murine embryonic stem cells and theoretically could be used to create or repair mutations in ...
Baltimore, David, Porteus, Matthew
core +2 more sources
Evolutionary and topological properties of gene modules and driver mutations in a leukemia gene regulatory network [PDF]
, 2015 The diverse, specialized genes in today's lifeforms evolved from a common core of ancient, elementary genes. However, these genes did not evolve individually: gene expression is controlled by a complex network of interactions, and alterations in one gene may drive reciprocal changes in its proteins' binding partners.
arxiv +1 more source
Retargeted adenoviruses for radiation-guided gene delivery [PDF]
, 2016 The combination of radiation with radiosensitizing gene delivery or oncolytic viruses promises to provide an advantage that could improve the therapeutic results for glioblastoma. X-rays can induce significant molecular changes in cancer cells.
A Beetz +54 more
core +2 more sources
A Simple Model for Gene Targeting [PDF]
Biophysical Journal, 2001 Sequence-specific binding to genomic-size DNA sequences by artificial agents is of major interest for the development of gene-targeting strategies, gene-diagnostic applications, and biotechnical tools. The binding of one such agent, peptide nucleic acid (PNA), to a randomized human genome has been modeled with statistical mass action calculations. With
Per Lincoln +2 more
openaire +3 more sources
Novel Heterotypic Rox Sites for Combinatorial Dre Recombination Strategies
G3: Genes, Genomes, Genetics, 2016 Site-specific recombinases (SSRs) such as Cre are widely used in gene targeting and genetic approaches for cell labeling and manipulation. They mediate DNA strand exchange between two DNA molecules at dedicated recognition sites. Precise understanding of
Katherine Chuang +3 more
doaj +1 more source
The magnetofection method: Using magnetic force to enhance gene delivery [PDF]
, 2003 In order to enhance and target gene delivery we have previously established a novel method, termed magnetofection, which uses magnetic force acting on gene vectors that are associated with magnetic particles.
Alexiou C. +10 more
core +3 more sources
Targeting in Gene Therapy for Gliomas [PDF]
Archives of Neurology, 1999 Cancer is a disease of a series of genes. Thus, theoretically, brain tumors could be treated by targeting their fundamental molecular defects. Currently, most of the approved clinical protocols for gene therapy involve cancer patients. Several of these protocols are designed to improve the treatment of brain tumors. In this brief report, we analyze the
Fueyo, J. +3 more
openaire +4 more sources
BMC Medical Genetics, 2002 Background SFHR (small fragment homologous replacement)-mediated targeting is a process that has been used to correct specific mutations in mammalian cells. This process involves both chemical and cellular factors that are not yet defined.
Bonifazi Emanuela +7 more
doaj +1 more source