Results 81 to 90 of about 12,260,520 (401)

Ezh2-dCas9 and KRAB-dCas9 enable engineering of epigenetic memory in a context-dependent manner. [PDF]

, 2019
BackgroundRewriting of the epigenome has risen as a promising alternative to gene editing for precision medicine. In nature, epigenetic silencing can result in complete attenuation of target gene expression over multiple mitotic divisions.
Bates, Sofie L, Carter, Sakereh S, Farnham, Peggy J, Fink, Kyle D, Halmai, Julian, Nisson, Karly A, O'Geen, Henriette, Rhie, Suhn K, Segal, David J   +8 more
core   +2 more sources

Gene targeting using the Agrobacterium tumefaciens-mediated CRISPR-Cas system in rice

Rice, 2014
BackgroundThe type II clustered, regularly interspaced, short palindromic repeat (CRISPR)/ CRISPR-associated protein 9 (Cas9) system is a novel molecular tool for site-specific genome modification.
R. Xu, Hao Li, R. Qin, Luping Wang, Li Li, P. Wei, Jianbo Yang   +6 more
semanticscholar   +1 more source

Autophagy in cancer and protein conformational disorders

FEBS Letters, EarlyView.
Autophagy plays a crucial role in numerous biological processes, including protein and organelle quality control, development, immunity, and metabolism. Hence, dysregulation or mutations in autophagy‐related genes have been implicated in a wide range of human diseases.
Sergio Attanasio
wiley   +1 more source

Gene-carried hepatoma targeting complex induced high gene transfection efficiency with low toxicity and significant antitumor activity

International Journal of Nanomedicine, 2012
Qing-Qing Zhao,1,2 Yu-Lan Hu,1 Yang Zhou,3 Ni Li,1 Min Han,1 Gu-Ping Tang,4 Feng Qiu,2 Yasuhiko Tabata,5 Jian-Qing Gao,11Institute of Pharmaceutics, Zhejiang University, Hangzhou, China; 2Department of Pharmacy, The First Affiliated Hospital of Chongqing
Zhao QQ, Hu YL, Zhou Y, Li N, Han M, Tang GP, Qiu F, Tabata Y, Gao JQ   +8 more
doaj  

A Myelin Proteolipid Protein-LacZ Fusion Protein Is Developmentally Regulated and Targeted to the Myelin Membrane in Transgenic Mice [PDF]

, 1993
Transgenic mice were generated with a fusion gene carrying a portion of the murine myelin proteolipid protein (PLP) gene, including the first intron, fused to the E. coli LacZ gene.
Duchala, Cynthia S., Macklin, Wendy B., Readhead, Carol, Wight, Patricia A.   +3 more
core   +2 more sources

Efficient Gene Targeting in Golden Syrian Hamsters by the CRISPR/Cas9 System

PLoS ONE, 2014
The golden Syrian hamster is the model of choice or the only rodent model for studying many human diseases. However, the lack of gene targeting tools in hamsters severely limits their use in biomedical research.
Z. Fan, Wei Li, Sang R. Lee, Q. Meng, Bi Shi, T. Bunch, K. White, I. Kong, Zhongde Wang   +8 more
semanticscholar   +1 more source

Protonophore activity of short‐chain fatty acids induces their intracellular accumulation and acidification

FEBS Letters, EarlyView.
The protonated form of butyrate, as well as other short‐chain fatty acids (SCFAs), is membrane permeable. In acidic extracellular environments, this can lead to intracellular accumulation of SCFAs and cytosolic acidification. This phenomenon will be particularly relevant in acidic environments such as the large intestine or tumor microenvironments ...
Muwei Jiang, Frans Bianchi, Geert van den Bogaart   +2 more
wiley   +1 more source

Flipping the cyclooxygenase (Ptgs) genes reveals isoform-specific compensatory functions1,2[S]

Journal of Lipid Research, 2018
Two prostaglandin (PG) H synthases encoded by Ptgs genes, colloquially known as cyclooxygenase (COX)-1 and COX-2, catalyze the formation of PG endoperoxide H2, the precursor of the major prostanoids.
Xinzhi Li, Liudmila L. Mazaleuskaya, Chong Yuan, Laurel L. Ballantyne, Hu Meng, William L. Smith, Garret A. FitzGerald, Colin D. Funk   +7 more
doaj  

The Gene Targeting Approach of Small Fragment Homologous Replacement (SFHR) Alters the Expression Patterns of DNA Repair and Cell Cycle Control Genes

Molecular Therapy: Nucleic Acids, 2016
Cellular responses and molecular mechanisms activated by exogenous DNA that invades cells are only partially understood. This limits the practical use of gene targeting strategies.
Silvia Pierandrei, Andrea Luchetti, Massimo Sanchez, Giuseppe Novelli, Federica Sangiuolo, Marco Lucarelli   +5 more
doaj   +1 more source

Solid-phase-assisted synthesis of targeting peptide-PEG-oligo(ethane amino)amides for receptor-mediated gene delivery. [PDF]

, 2012
In the forthcoming era of cancer gene therapy, efforts will be devoted to the development of new efficient and non-toxic gene delivery vectors. In this regard, the use of Fmoc/Boc-protected oligo(ethane amino)acids as building blocks for solid-phase ...
Akinc, Aumailley, Behr, Blessing, Boussif, Breunig, Carlos Mas-Moruno, Chollet, Christian Dohmen, Christina Troiber, Cotten, Cox, David Schaffert, Davis, Davis, Dechantsreiter, Desgrosellier, Drake, Edinger, Ellman, Erbacher, Ernest Giralt, Ernst Wagner, Felding-Habermann, Felgner, Fella, Grandinetti, Harbottle, Hartmann, Hartmann, Haubner, Horst Kessler, Irene Martin, J., Kantlehner, Kichler, Kim, Kursa, Li, Lin, Luthman, Madder, Mas-Moruno, McKenzie, Meritxell Teixidó, Merkel, Meyer, Meyer, Michael Günther, Moghimi, Mosca, Nie, Oba, Oupicky, Pack, Petra Kos, Pfaff, Plank, Reis, Ruoslahti, Russ, Russ, Schaffert, Schaffert, Schaffert, Schiffelers, Sonawane, Trubetskoy, Ulrich Lächelt, Varkouhi, Wagner, Wagner, Wagner, Wagner, Walker, Wu, Xia, Xu, Zintchenko   +78 more
core   +3 more sources